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2007 Fiscal Year Final Research Report Summary

Development of novel efficient methods for detecting SNP(Single Nucleotide Polymorphism)

Research Project

Project/Area Number 16350090
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionKanagawa University (2005-2007)
Tokyo Metropolitan University (2004)

Principal Investigator

ONO Akira  Kanagawa University, Faculty of Engineering, Professor (10183253)

Co-Investigator(Kenkyū-buntansha) OKAMOTO Itaru  Kanagawa University, Faculty of Engineering, assistant Professor (40460133)
TASHIRO Mitsuru  Meisei University, School of Science and Engineering, Professor (40315750)
Project Period (FY) 2004 – 2007
KeywordsSNP / single nucleotide polymorphism / DNA / metal ions / DNA duplex / thermal stability / mercury / silver
Research Abstract

The goal of the research is developing a new efficient methods for detecting single-nucleotide-polymorphism (SNP) using a phenomenon, certain metal ions highly selectively bind to miss base pairs in DNA duplexes, recently found in our laboratory. Mercury(II) and silver(I) ions highly selectively bind to thymine-thymine (T-T) pair and cytosine-cytosine (C-C) pair in DNA duplexes, respectively, and consequently the duplexes containing the miss base pairs are stabilized in the presence of the metal ions. The phenomenon is totally different form the existed methods in which metal ions are captured by artificial base pairs in DNA duplexes. This finding that the natural bases selectively bind to the certain metal ions is scientifically interesting, as well as this can be used for developing new SNP typing methods.
In this study, first of all, we examined a capability that a SNP typing method can be developed by detecting the increased thermal stability of a duplex in the presence of the metal ions. We used the isothermal titration calorimetric experiments, and some results were published. Also, we tried to detect SNP from the fluorescence emission intensity differences in the absence and in the presence of the metal ions.
Beside the application studies, we thermodynamically studied binding processes of the metal ions and the base pairs. We tried to clarify the structure of the metal ion binding sites, and NMR method was efficient for Hg(II)-DNA and Ag(I)-DNA complexes.
We have searched new metal ion-base pair combinations in which metal ions selectively and tightly bind to base pairs and DNA duplexes are stabilized in the presence of metal ions. However, such metal ion-base pair combinations were not found. Also, we searched new metal mediated base pairs in which the natural bases and artificial bases are tightly connected by metal ions, and the study has been in progress.

  • Research Products

    (38 results)

All 2007 2006 2005 2004 Other

All Journal Article (16 results) (of which Peer Reviewed: 4 results) Presentation (21 results) Book (1 results)

  • [Journal Article] ^<15>N-^<15>N J-coupling across Hg^<II>:Direct observation of Hg^<II>-mediated T-T base pairs in a DNA duplexes2007

    • Author(s)
      Yoshiyuki Tanaka, et. al.
    • Journal Title

      Journal of the American Chemical Society 129

      Pages: 245-246

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 15N-15N J-coupling across Hg^<II> : Direct observation of Hg^<II>-mediated T-T base pairs in a DNA duplex2007

    • Author(s)
      Yoshiyuki Tanaka*, Shuji Oda, Hiroshi Yamaguchi, Yoshinori Kondo, Chojiro Kojima, Akira Ono*
    • Journal Title

      J. Am. Chem. Soc. 129

      Pages: 244-245

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mercury^<II>-mediated formation of thymine-Hg^<II>-thymine base pairs in DNA duplexes2006

    • Author(s)
      Yoko Miyake, et. al.
    • Journal Title

      Journal of the American Chemical Society 128

      Pages: 2172-2173

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] NMR spectroscopic study of a DNA duplex with mercury-mediated T-T base pairs2006

    • Author(s)
      Yoshiyuki Tanaka, et. al.
    • Journal Title

      Nucleoside Nucleotides & Nucleic Acids 25

      Pages: 613-624

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Mercury^<II>-mediated formation of thymine-Hg^<II>-thymine base pairs in DNA duplexes2006

    • Author(s)
      Yoko Miyake, Humika Togashi, Mitsuru Tashiro, Hiroshi Yamaguchi, Shuji Oda, Megumi Kudo, Yoshiyuki Tanaka, Yoshinori Kondo, Ryuichi Sawa, Takashi Fuiimoto, Tomoya Machinami, Akira Ono
    • Journal Title

      J. Am. Chem. Soc. 128

      Pages: 2172-2173

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] NMR spectroscopic study of a DNA duplex with mercury-mediated T-T base pairs2006

    • Author(s)
      Yoshiyuki Tanaka, Hiroshi Yamaguchi, Shuji Oda, Makoto Nomura, Chojiro Kojima, Yoshinori Kondo, Akira Ono
    • Journal Title

      Nucleoside Nucleotides & Nucleic Acids 25

      Pages: 613-624

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Fluorescent Sensor for Redox-Environment:A Redox-Controlled Molecular Device Based on the Reversible Mercury Mediated Folded Structure Formation of Oligothymidylate2005

    • Author(s)
      Yoko Miyake and Akira Ono
    • Journal Title

      Tetrahedron Letters 46

      Pages: 2441-2443

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Fluorescent Sensor for Redox-Environment : A Redox-Controlled Molecular Device Based on the Reversible Mercury Mediated Folded Structure Formation of Oligothymidylate2005

    • Author(s)
      Yoko Miyake, Akira Ono.
    • Journal Title

      Tetrahedron Lett. 46

      Pages: 2441-2443

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Metal ion binding of substituted pyrimidine base pairs in DNA duplexes2004

    • Author(s)
      Yuko Watanabe, Takahiro Minowa and Akira Ono
    • Journal Title

      Nucleic Acids Symposium Series 48

      Pages: 85-86

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Silver(I) cation specifically stabilizes heteroduplex with C:C mismatch base pair:toward the efficient detection of single nucleotide polymorphism(2)2004

    • Author(s)
      Hidetaka Torigoe, Akira Ono and Ayako Takamori
    • Journal Title

      Nucleic Acids Symposium Series 48

      Pages: 101-102

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Spectroscopic analysis of DNA duplexes including T-T mismatches2004

    • Author(s)
      Hiroshi Yamaguchi, Shuji Oda, Yoshinori Kondo, Akira Ono and Yoshiyuki Tanaka
    • Journal Title

      Nucleic Acids Symposium Series 48

      Pages: 113-114

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Thermodynamic analyses of the specific interaction between T:T mismatch base pair and mercury(II)cation:Toward the efficient detection of single nucleotide polymorphism(1)2004

    • Author(s)
      Hidetaka Torigoe, Akira Ono and Kouji Kawahashi
    • Journal Title

      Nucleic Acids Symposium Series 48

      Pages: 275-276

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Metal ion binding of substituted pyrimidine base pairs in DNA duplexes2004

    • Author(s)
      Y. Watanabe, T. Minowa, A. Ono
    • Journal Title

      Nucleic Acids Symposium Series No.48

      Pages: 85-86

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Silver(I) cation specifically stabilizes heteroduplex with C : C mismatch base pair : toward the efficient detection of single nucleotide polymorphism(2)2004

    • Author(s)
      T. Torigoe, A. Ono, A. Takamori
    • Journal Title

      Nucleic Acids Symposium Series No.48

      Pages: 101-102

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Spectroscopic analysis of DNA duplexes including T-T mismatches2004

    • Author(s)
      H. Yamaguchi, S. Oda, Y. Kondo, A. Ono, Y. Tanaka
    • Journal Title

      Nucleic Acids Symposium Series No.48

      Pages: 113-114

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Thermodynamic analyses of the specific interaction between T : T mismatch base pair and mercury(II) cation : Toward the efficient detection of single nucleotide polymorphism(1)

    • Author(s)
      H. Torigoe, A. Ono, K. Kawahashi
    • Journal Title

      Nucleic Acids Symposium Series No.48

      Pages: 275-276

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The specific interaction between two C:C mismatch base pairs and silver(I) cation2007

    • Author(s)
      H.Torigoe, T.Kozasa, A.Ono
    • Organizer
      5th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Tokyo
    • Year and Date
      20071120-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The specific interactoin between two T:T mismatch base pairs and mercury(II) cation2007

    • Author(s)
      H.Torigoe, Y.Miyakawa, T.Kozasa, A.Ono
    • Organizer
      5th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Tokyo
    • Year and Date
      20071120-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The specific interaction between two C : C mismatch base pairs and silver(I) cation2007

    • Author(s)
      H. Torigoe, T. Kozasa, A. Ono
    • Organizer
      5th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Tokyo
    • Year and Date
      20071120-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The specific interactoin between two T : T mismatch base pairs and mercury(II) cation2007

    • Author(s)
      H. Torigoe, Y. Miyakawa, T. Kozasa, A. Ono
    • Organizer
      5th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Tokyo
    • Year and Date
      20071120-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] DNA二重らせん構造中における水銀(II)を介したチミンの塩基対形成2007

    • Author(s)
      田中 好幸・織田 修司・小室 朋之・根東 義則・鳥越 秀峰・小野 晶
    • Organizer
      日本化学会第87春季年会
    • Place of Presentation
      関西大学
    • Year and Date
      20070325-28
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Thermodynamic analyses of the specific interaction between two C:C mismatch base pairs and silver(I) cations2006

    • Author(s)
      Hidetaka Torigoe, Yukako Miyakawa, Nobuyuki Nagasawa, Tetsuo Kozasa and Akira Ono
    • Organizer
      33rd Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Osaka
    • Year and Date
      20061120-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Detection of T:T mismatch base pair by fluorescence spectral change upon addition of mercury(II) cation:Toward the efficient analyses of single nucleotide polymorphism2006

    • Author(s)
      Hidetaka Torigoe, Tetsuo Kozasa, Akira Ono
    • Organizer
      33rd Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Osaka
    • Year and Date
      20061120-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Detection of C:C mismatch base pair by fluorescence spectral change upon addition of silver(I)cation:Toward the efficient analyses of single nucleotide polymorphism2006

    • Author(s)
      Hidetaka Torigoe, Tetsuo Kozasa and Akira Ono
    • Organizer
      33rd Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Osaka
    • Year and Date
      20061120-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Thermodynamic analyses of the specific interaction between two C : C mismatch base pairs and silver(I) cations2006

    • Author(s)
      Hidetaka Torigoe, Yukako Miyakawa, Nobuyuki Nagasawa, Tetsuo Kozasa, Akira Ono
    • Organizer
      33rd Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Osaka
    • Year and Date
      20061120-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Detection of T : T mismatch base pair by fluorescence spectral change upon addition of mercury(II) cation : Toward the efficient analyses of single nucleotide polymorphism2006

    • Author(s)
      Hidetaka Torigoe, oTetsuo Kozasa, Akira Ono
    • Organizer
      33rd Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Osaka
    • Year and Date
      20061120-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Detection of C : C mismatch base pair by fluorescence spectral change upon addition of silver(I) cation : Toward the efficient analyses of single nucleotide polymorphism2006

    • Author(s)
      Hidetaka Torigoe, Tetsuo Kozasa, Akira Ono
    • Organizer
      33rd Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Osaka
    • Year and Date
      20061120-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The Synthesis of DNA-Wires Containing Metal Ions2006

    • Author(s)
      A.Ono, K.Sugiyama, T.Nishiuchi, Y.Watanabe, T.Minowa
    • Organizer
      XVII International Round Table
    • Place of Presentation
      Bern,Switzerland
    • Year and Date
      20060903-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The Synthesis of DNA-Wires Containing Metal Ions2006

    • Author(s)
      A. Ono, K. Sugiyama, T. Nishiuchi, Y. Watanabe, T. Minowa
    • Organizer
      XVII International Round Table(IRT-XVII)
    • Place of Presentation
      Bern, Switzerland
    • Year and Date
      20060903-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Synthesis of Novel Metal Ion Sensors Based on DNA-Metal Interactions2005

    • Author(s)
      Akira Ono, Shiqi Cao, Humika Togashi, Yoko Miyak
    • Organizer
      15th International Symposium of Fine Chemistry and Functional Polymers
    • Place of Presentation
      Shanghai,China
    • Year and Date
      20051017-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Synthesis of Novel Metal Ion Sensors Based on DNA-Metal Interactions2005

    • Author(s)
      Akira Ono, Shiqi Cao, Humika Togashi, Yoko Miyake
    • Organizer
      15th International Symposium of Fine Chemistry and Functional Polymers(FCFP-XV) & IUPAC 1th International Symposium on Novel Materials and Synthesis(NMS-I)
    • Place of Presentation
      Shanghai, China
    • Year and Date
      20051017-20
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Thermodynamic analyses of the specific interaction between C:C mismatch base pair and silver(I)cation:Toward the efficient detection of single nucleotide polymorphism2005

    • Author(s)
      Hidetaka Torigoe, OTetsuo Kozasa, Ayako Takamori and Akira Ono
    • Organizer
      4th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Fukuoka
    • Year and Date
      20050920-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Thermodynamic analyses of the specific interaction between C : C mismatch base pair and silver(I) cation : Toward the efficient detection of single nucleotide polymorphism2005

    • Author(s)
      Hidetaka Torigoe, oTetsuo Kozasa, Ayako Takamori, Akira Ono
    • Organizer
      4th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Fukuoka
    • Year and Date
      20050920-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] DNA二重らせん中におけるチミン-水銀-チミン(T-Hg-T)塩基対形成とNMRによる構造解析2005

    • Author(s)
      田中 好幸, 他
    • Organizer
      第125回日本薬学会
    • Place of Presentation
      東京
    • Year and Date
      20050329-31
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] ミスマッチ塩基対CCと銀(I)イオンとの特異的な結合に関する熱力学的解析-1 塩基多型の効率的検出に向けて-2005

    • Author(s)
      高森 絢子, 他
    • Organizer
      日本化学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20050326-29
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Development of novel oligonucleotide-based sensors which are highly Hg(II) selective and are insensitive to other heavy metal ions2004

    • Author(s)
      小野 晶
    • Organizer
      第31回核酸化学シンポジウム
    • Place of Presentation
      昭和大学薬学部(東京)
    • Year and Date
      20041110-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Development of novel oligonucleotide-based sensors which are highly Hg(II) selective and are insensitive to other heavy metal ions2004

    • Author(s)
      A. Ono
    • Organizer
      Therty-first Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Tokyo
    • Year and Date
      20041110-12
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Moleculomics and Thereafte2006

    • Author(s)
      Torigoe, H.and Ono, A.
    • Total Pages
      1-21
    • Publisher
      Research Signpost,Ltd
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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