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2006 Fiscal Year Final Research Report Summary

Preparation and the function of a novel surfactant vesicle with a recognition ability of the specific antigen of cancer cell toward practical clinical application

Research Project

Project/Area Number 16360415
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionEhime University

Principal Investigator

KATO Keiichi  Ehime University, Graduate School of Science and Engineering, Associate Professor, 理工学研究科, 助教授 (10117088)

Co-Investigator(Kenkyū-buntansha) SUGAHARA Takuya  Ehime University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (00263963)
AKIYAMA Koichi  Ehime University, Integrated Center for Sciences, Assistant Professor, 総合科学研究支援センター, 助手 (80231835)
MASUDA Seizo  Ehime University, Integrated Center for Sciences, Senior Assistant Professor, 総合科学研究支援センター, 講師 (50363263)
SUZUKI Yoji  Ehime University, Graduate School of Medicine, Senior Assistant Professor, 医学系研究科, 講師 (20226567)
Project Period (FY) 2004 – 2006
KeywordsDDS / Cancer therapy / Apoptosis / Span80 / Liposome / Immunovesicle / Alga lectin / Gene transfection
Research Abstract

The main purpose of this work is the practical clinical application of the novel vesicle for a cancer therapy in drug delivery system (DDS), preparing a non-ionic surfactant vesicle, composed of Span80 (sorbitan monooleate). As the immobilized ligand as a "tumor-targeting missile device" on the Span80 vesicle, two species of ligands were chosen: (1) novel alga lectin ESA (Eucheuma Serra agglutinin:) and (2) monochronal antibody against the antigen of a tumor cell.
We revealed that Span80 was a surfactant mixture of sorbitan ester isomers of mono-, di-tri-and tetra-esters and the vesicle membrane was constructed as constant composition of the isomers by a thermodynamic self-assembly. The main component was diester (about 60%). Span80 vesicle had higher fluidity and superior characteristics such as membrane fusion, heat-satiability, metabolism and low-cost, compared with usual liposome with natural phospholipids. The ESA-immobilized Span80-vesicle (ESA vesicle) was revealed to specifically combine to cancer cells, such as colon adenocarcinoma (Colo201, Colon26) in vivo. We could also reveal that the ESA vesicle, injected to a mouse with the colon adenocarcinomas, transferred through the blood wall and targeted at the tumor following by the apoptotic death (confirmed by TUNNEL analysis) of the tumor. The vesicle accumulated in the liver was perfectly metabolized within 48 hours. The Span80 vesicle containing anticancer drug of docetaxcel (TXT) could perfectly controlled the mouse tumor. The influence of the vesicle size and the side effect could not be observed. The apoptotic mechanism (through Caspae3 and 8) was also clarified by genome-analysis. We could also demonstrate the effective control of the growth of the model human brain-tumor (from ERM5-1 cell) by injecting the immunovesicle immobilized with anti-EGFR antibody. Moreover, using the immunovesicle as a vector, gene transfection to the ERM5-1 tumor in vivo was successfully performed.

  • Research Products

    (28 results)

All 2007 2006 2005 2004 Other

All Journal Article (23 results) Book (5 results)

  • [Journal Article] Development of drug delivery carrier and gene transfection vector by using surfactant nano-vesicle : Introductory interfacial chemistry2007

    • Author(s)
      Keiichi Kato
    • Journal Title

      Chemistry and the Education(Chemical Society of Japan) Vol.55, No.4

      Pages: 184-187

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular composition of nonionic vesicles prepared from Span 80 or Span 85 by a two-step emulsification method2006

    • Author(s)
      Keiichi Kato
    • Journal Title

      Journal of Dispersion Science and Technology 27, 8

      Pages: 1217-1222

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 癌臨床応用をめざすドラッグデリバリーシステム2006

    • Author(s)
      加藤 敬一
    • Journal Title

      愛媛大学工学ジャーナル 5

      Pages: 79-88

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The anti-tumor effect of Euchema serra agglutinin (ESA) on colon cancer cells in vitro and in vivo.2006

    • Author(s)
      Keiichi, Kato
    • Journal Title

      Anti-Cancer Drugs 17

      Pages: 943-947

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Antitumor effect of novel-lectin-immobilized vesicle, mainly composed of non-ionic surfactant Span80, against the human colon tumor transplanted to a nude mouse2006

    • Author(s)
      Keiichi, Kato
    • Journal Title

      Proc. of 6th European Symposium on Biochemical Engineering Science

      Pages: 113

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Antitumor effect of novel-lectin-immobilized vesicle of nonionic surfactant against Colon26 tumor transplanted to mouse with autoimmune function2006

    • Author(s)
      Keiichi, Kato
    • Journal Title

      Proc. of 6th European Symposium on Biochemical Engineering Science

      Pages: 259

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular composition of nonionic vesicles prepared from Span 80 or Span 85 by a two-step emulsification method2006

    • Author(s)
      Keiichi Kato
    • Journal Title

      Journal of Dispersion Science and Technology 27,8

      Pages: 1217-1222

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Drug delivery system toward for clinical application of cancer therapy2006

    • Author(s)
      Keiichi Kato
    • Journal Title

      Annual Journal of Engineering Ehime University Vol5

      Pages: 79-88

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The anti-tumor effect of Euchema serra agglutinin (ESA) on colon cancer cells in vitro and in vivo.2006

    • Author(s)
      Yuuki Fukuda
    • Journal Title

      Anti-Cancer Drugs 17

      Pages: 943-947

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Antitumor effect of novel-lectin- immobilized vesicle, mainly composed of non-ionic surfactant Span80, against the human colon tumor transplanted to a nude mouse2006

    • Author(s)
      Keiichi Kato
    • Journal Title

      Proc.of 6th European Symposium on Biochemical Engineering Science

      Pages: 113

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Antitumor effect of novel-lectin immobilized vesicle of nonionic surfactant against Colon26 tumor transplanted to mouse with autoimmune function2006

    • Author(s)
      Keiichi Kato
    • Journal Title

      Proc.of 6th European Symposium on Biochemical Engineering Science

      Pages: 259

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Gene transfection into Hela cells by vesicles containing cationic peptide lipid2005

    • Author(s)
      Yumi Ohama
    • Journal Title

      Bioscience, Biotechnology and Biochemistry 69, 8

      Pages: 1453-1458

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Preparation of cationic immunovesicles containing cationic peptide lipid for specific drug delivery to target cells2005

    • Author(s)
      Takuya Sugahara
    • Journal Title

      Cytotechnology 47

      Pages: 51-57

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Nano-size vesicle used in drug carrier and nano-capsule-Missile attack and gene transfection of a tumor by nano artificial cell-2005

    • Author(s)
      Keiichi Kato
    • Journal Title

      Biotechnology Journal(Yohdosya) 5(3)

      Pages: 306-309

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Structure of non-ionic surfactant Span80 vesicle and the application2005

    • Author(s)
      Keiichi, Kato
    • Journal Title

      New development of liposome engineering(NTS syuppan) No.3, Chapter8

      Pages: 407-414

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Anti-cancer effect to colon cancer in either vitro or vivo using lipid vesicle combined with alga lecithin ESA2004

    • Author(s)
      Keiichi Kato
    • Journal Title

      Proc. of the 10^<th> Asian Pacific Confederation of Chem. Eng. (APCChE)

      Pages: 1P-01-032

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Specific targeting at ERM5-1 cell as a model human-brain tumor cell and the anti-tumor effect by using immunovesicle2004

    • Author(s)
      Keiichi Kato
    • Journal Title

      Proc. of the 10^<th> Asian Pacific Confederation of Chem. Eng. (APCChE)

      Pages: 1P-01-033

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ドラッグデリバリーシステムによる癌治療および遺伝子導入2004

    • Author(s)
      加藤 敬一
    • Journal Title

      日本エム・イー学会誌 生体医工学 第42巻特別号II

      Pages: 185

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Anti-cancer effect to colon cancer in either vitro or vivo using lipid vesicle combined with alga lecithin ESA2004

    • Author(s)
      Keiichi Kato
    • Journal Title

      Proc.of the 10^<th> Asian Pacific Confederation of Chem.Eng. (APCChE)

      Pages: 1P-01-032

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Specific targeting at ERM5-1 cell as a model human-brain tumor cell and the anti-tumor effect by using immunovesicle2004

    • Author(s)
      Keiichi Kato
    • Journal Title

      Proc.of the 10^<th> Asian Pacific Confederation of Chem.Eng. (APCChE)

      Pages: 1P-01-033

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cancer therapy and gene trans- fection in drug delivery system2004

    • Author(s)
      Keiichi Kato
    • Journal Title

      Transactions of the Japanese Soc. for medical and biological engineering, Japan Soc.of ME & BE 42(11)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Preparation of the W/O/W emulsion and the application-Structure, size distribution and application to biochemical engineering2004

    • Author(s)
      Keiichi, Kato
    • Journal Title

      New emulsification techniques, Johhou Kikou, Chapter 4

      Pages: 67-80

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Application of non-ionic surfactant Span80 vesicle to drug delivery system

    • Author(s)
      Keiichi Kato
    • Journal Title

      Eco-material handbook(Maruzen) (to be published)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 化学と教育2007

    • Author(s)
      加藤 敬一
    • Total Pages
      204(4)
    • Publisher
      日本化学会
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] エコマテリアルハンドブック-非イオン性界面活性剤Span80ベシクルのドラッグデリバリーシステムへの利用-2007

    • Author(s)
      加藤 敬一
    • Total Pages
      846
    • Publisher
      丸善(株)
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] リポソーム応用の新展開 : (第3編8章;非イオン性界面活性剤Span80ベシクルの構造と利用)2005

    • Author(s)
      加藤 敬一
    • Total Pages
      683(7)
    • Publisher
      NTS出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] バイオテクノロジージャーナル : (ナノ人工細胞による癌細胞のミサイル攻撃・遺伝子導入)2005

    • Author(s)
      加藤 敬一
    • Total Pages
      376(3)
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 新しい乳化技術 : (第4章、W/O/Wエマルションの調製とその利用 -構造・粒径分布・生物工学的利用-)2004

    • Author(s)
      加藤 敬一
    • Total Pages
      285(14)
    • Publisher
      情報機構
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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