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2005 Fiscal Year Final Research Report Summary

Molecular dissection of the 26S proteasome in the budding yeast

Research Project

Project/Area Number 16370001
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionThe University of Tokyo

Principal Investigator

TOH-E Akio  The University of Tokyo, Graduate School of Sciences, Professor, 大学院・理学系研究科, 教授 (90029249)

Project Period (FY) 2004 – 2005
Keywords26S proteasome / lid / base / temperature-sensitive mutants / hierarchy of assembly / 19SRP / yeast
Research Abstract

It is widely accepted that the ubiquitin-proteasome system playas a pivotal role in degradation of short-lived proteins, many of them are regulatory proteins, in an energy dependent manner. The 26S proteasome is responsible to the final step of proteolysis in this system. The 26S proteasome is a large protein complex consisting of 20S proteasome containing active sites and 19S regulatory factor called as 19S RP. The basic subunit composition has been elucidated. By exploiting this information, we started uncovering how and where these subunits assemble together. We focused on the 19S RP of Saccharomyces cerevisiae. The 19S RP consisted of two sub-complexes, the lid and the base. We isolated temperature-sensitive mutants of the genes encoding subunits of the lid.Biochemical characterization revealed that hierarchy of the assembly of the lid and the lid and the base are constructed independently of each other. We also examined localization of each sub-complex in various mutants and found that the base and the lid can separately be localized in the nucleus. The 19S RP too is localized in the nucleus and assembles with the 20S proteasome to the 26S proteasome.

  • Research Products

    (26 results)

All 2005 2004

All Journal Article (26 results)

  • [Journal Article] Las24/KOGI,a component of the TOR complex 1(TORC1), is needed for resistance to local anesthetic tetracaine and normal distribution of actin cytoskeleton in yeast.2005

    • Author(s)
      Araki, T.
    • Journal Title

      Genes Genet. Syst. 80

      Pages: 325-343

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Functional analysis of Rpn6p, a lid component of the 26S proteasome, using temperature-sensitive rpn6 mutants of the yeast Saccharomyces cerevisiae.2005

    • Author(s)
      Isono, E.
    • Journal Title

      J. Biol. Chem. 280

      Pages: 6537-6547

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Knocking out ubiquitin proteasome system function in vivo and in vitro with genetically encodable tandem ubiquitin.2005

    • Author(s)
      Saeki, Y.
    • Journal Title

      Method. Enzymol. 399

      Pages: 64-74

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity.2005

    • Author(s)
      Saeki, Y.
    • Journal Title

      Method. Enzymol. 399

      Pages: 215-227

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Las24/KOG1, a component of the TOR complex 1(TORC1), is needed for resistance to local anesthetic tetracaine and normal of distribution of actin cytoskeleton in yeast.2005

    • Author(s)
      Araki, T.
    • Journal Title

      Genes Genet.Syst. 80

      Pages: 325-343

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Functional analysis of Rpn6p, a lid componenet of the 26S proteasome, using temperature-sensitive rpn6 mutants of the yeast Saccharomyces cerevisiae.2005

    • Author(s)
      Isono, E.
    • Journal Title

      J.Biol.Chem. 280

      Pages: 6537-6547

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Knocking out ubiquitin proteasome system function in vivo and in vitro with genetically encodable tandem ubiquitin.2005

    • Author(s)
      Saeki, Y.
    • Journal Title

      Method.Enzymol 399

      Pages: 64-74

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity.2005

    • Author(s)
      Saeki, Y.
    • Journal Title

      Method.Enzymol 399

      Pages: 215-227

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Rpn7 is required for the structural integrity of the 26S proteasome of Saccharomyces cervisiae.2004

    • Author(s)
      Isono, E.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 27168-27176

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Definitive evidence for Ufd2-catalyzed elongation of the ubiquitin chain through Lys48 linkage.2004

    • Author(s)
      Saeki, Y.
    • Journal Title

      Biochem. Biophys. Res. Commun. 320

      Pages: 840-845

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division.2004

    • Author(s)
      Saeki, Y.
    • Journal Title

      Genes Genet. Syst. 79

      Pages: 77-86.

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Semlp is a novel subunit of the 26S proteasome from Saccharomyces cerevisiae.2004

    • Author(s)
      Sone, T.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 28807-28816

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae.2004

    • Author(s)
      Iwase, M.
    • Journal Title

      Cell Struc. Funct. 29

      Pages: 1-15

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Simultaneous yet independent regulation of actin cytoskeletal organization and translation initiation by glucose in Saccharomyces cerevisiae.2004

    • Author(s)
      Uesono, Y.
    • Journal Title

      Mol. Biol. Cell 15

      Pages: 1544-1556

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mmrlp is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast2004

    • Author(s)
      Itoh, T.
    • Journal Title

      EMBO J. 23

      Pages: 2520-2530

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Forchlorfenuron, a phenylurea cytokinin, disturbs septin organization in Saccharomyces cerevisiae.2004

    • Author(s)
      Iwase, M.
    • Journal Title

      Genes Genet. Syst. 79

      Pages: 199-206

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Yeast Pho85 kinase is required for proper gene expression during the diauxic shift.2004

    • Author(s)
      Nishizawa, M.
    • Journal Title

      Yeast 21

      Pages: 903-918

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Rpn7 is required for the structural integrity of the 26S proteasome of Saccharomyces cervisiae.2004

    • Author(s)
      Isono, E.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 27168-27176

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Definitive evidence for Ufd2-catalyzed elongation of the ubiquitin chain through Lys48 linkage.2004

    • Author(s)
      Saeki, Y.
    • Journal Title

      Biochem.Biophys.Res.Commun. 320

      Pages: 840-845

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division.2004

    • Author(s)
      Saeki, Y.
    • Journal Title

      Genes Genet.Syst. 79

      Pages: 77-86

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Semlp is a novel subunit of the 26S proteasome from Saccharomyces cerevisiae.2004

    • Author(s)
      Sone, T
    • Journal Title

      J.Biol.Chem. 279

      Pages: 28807-28816

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae.2004

    • Author(s)
      Iwase, M.
    • Journal Title

      Cell Struc.Funct. 29

      Pages: 1-15

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Simultaneous yet independent regulation of actin Cytoskeletal organization and translation initiation by glucose in Saccharomyces cerevisiae.2004

    • Author(s)
      Uesono, Y.
    • Journal Title

      Mol.Biol.Cell 15

      Pages: 1544-1556

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast.2004

    • Author(s)
      Itoh, T.
    • Journal Title

      EMBO J. 23

      Pages: 2520-2530

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Forchlorfenuron, a phenylurea cytokinin, disturbs septin organization in Saccharomyces cerevisiae.2004

    • Author(s)
      Iwase, M.
    • Journal Title

      Genes Genet.Syst. 79

      Pages: 199-206

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Yeast Pho85 kinase is required for proper gene expression during the diauxic shift.2004

    • Author(s)
      Nishizawa
    • Journal Title

      Yeast 21

      Pages: 903-918

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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