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2006 Fiscal Year Final Research Report Summary

Molecular mechanisms of synthesis, transport, and accumulation of glycinebetaine in plants and cyanobacteria

Research Project

Project/Area Number 16370028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionMeijo University

Principal Investigator

TAKABE Teruhiro  Meijo University, Research Institute, Professor, 総合研究所, 教授 (80097766)

Co-Investigator(Kenkyū-buntansha) TANAKA Yoshito  Meijo University, Faculty of Science & Technology, Associate Professor, 理工学部, 助教授 (10247679)
HIBINO Takashi  Meijo University, Faculty of Science & Technology, Associate Professor, 理工学部, 助教授 (70218741)
NAKAMURA Tatsunosuke  Niigata University, Pharmacy & Applied Life Science, Professor, 薬学部, 教授 (20114308)
SHIKATA Masamitsu  Shimadzu Co., Genomic research, Director, 分析計測事業部ジェノミックリサーチ室, 副主任研究員 (20374126)
Project Period (FY) 2004 – 2006
KeywordsGrovel environment / Food / Salt stress / Glycinebetaine / Genetic engineering / Plants / Halotolerant cyanobacterium / Transport
Research Abstract

Glycine betaine (betaine) is a important osmoprotectant which accumulates under abiotic stresses. In this projects, we have studied molecular mechanisms of synthesis, transport and accumulation of betaine in plantas and halotolerant cyanobacteria. In this project, we obtained the following results. 1) Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis. 2) Isolation and functional characterization of Ca^<2+>/H^+ antiporters from cyanobacteria. 3) Isolation and functional characterization of betaine transporters from halotolerant cyanobacterium and mangrove. 4) Cloning, functional expression and primary characterization of Vibrio parahaemolyticus K^+/H^+ antiporter Escherichia coli ChaA. 4) Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity. 5) Carboxyl-terminal hydrophilic tail of a NhaP type Na^+/H^+ antiporter from cyanobacteria is involved in the apparent affinity for Na^+ and pH sensitivity. 6) Root specific expression of Na^+/H^+ antiporter gene from Synechosystis sp. PCC6803 confers salt tolerance of tobacco plant.

  • Research Products

    (34 results)

All 2006 2005 2004

All Journal Article (28 results) Book (6 results)

  • [Journal Article] Cloning, functional expression and primary characterization of Vibrio parahaemolyticus K^+/H^+ antiporter genes in Escherichia coil.2006

    • Author(s)
      Radchenko MV, Waditee R, Oshimi S, Fukuhara M, Takabe T, Nakamura T
    • Journal Title

      Mol. Microbiol. 59

      Pages: 651-663

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Potassium/proton antiport system of Escherichia coli.2006

    • Author(s)
      Radchenko MV, Tanaka K, Waditee R, Oshimi S, Matsuzaki Y, Fukuhara M, Kobayashi H, Takabe T, Nakamura T
    • Journal Title

      J. Biol. Chem. 281

      Pages: 19822-19829

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity.2006

    • Author(s)
      Laloknam S, Tanaka K, Buaboocha T, Waditee R, Incharoensakdi A, Hibino T, Tanaka Y, Takabe T
    • Journal Title

      Appl. Environ. Microbiol 72

      Pages: 6018-6026

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Carboxyl-terminal hydrophilic tail of a NhaP type Na^+/H^+ antiporter from cyanobacteria is involved in the apparent affinity for Na+ and pH sensitivity.2006

    • Author(s)
      Waditee R, Buaboocha T, Kato M, Hibino T, Suzuki S, Nakamura T, Takabe T
    • Journal Title

      Arch. Biochem. Biophys. 450

      Pages: 113-121

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Root specific expression of Na^+/H^+ antiporter gene from Synechosystis sp. PCC6803 confers salt tolerance of tobacco plant.2006

    • Author(s)
      Hossain GS, Waditee R, Hibino T, Tanaka Y, Takabe T
    • Journal Title

      Plant Biotechnol 23

      Pages: 275-281

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Conformational changes during apoplastocyanin folding observed by photocleavable modification and transient grating.2006

    • Author(s)
      Hirota S, Fujimoto Y, Choi J, Baden N, Katagiri N, Akiyama M, Hulsker R, Ubbink M, Okajima T, Takabe T, Funasaki N, Watanabe Y, Terazima M
    • Journal Title

      J. Am. Chem. Soc. 128

      Pages: 7551-7558

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reduction of plastocyanin by tyrosine-containing oligopeptides.2006

    • Author(s)
      Hirota S, Okumura H, Kondoh T, Funasaki N, Takabe T, Watanabe Y
    • Journal Title

      J. Inorg. Biochem 100

      Pages: 1871-1878

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Potassium/proton antiport system of Escherichia coli2006

    • Author(s)
      Radchenko MV, Tanaka K, Waditee R, Oshimi S, Matsuzaki Y, Fukuhara M, Kobayashi H, Takabe T, Nakamura T.
    • Journal Title

      J.Biol.Chem. 281

      Pages: 19822-19829

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity2006

    • Author(s)
      Laloknam S, Tanaka K, Buaboocha T, Waditee R, Incharoensakdi A, Hibino T, Tanaka Y, Takabe T.
    • Journal Title

      Appl.Environ.Microbiol. 72

      Pages: 6018-6026

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Carboxyl-terminal hydrophilic tail of a NhaP type Na^+/H^+ antiporter from cyanobacteria is involved in the apparent affinity for Na+ and pH sensitivity2006

    • Author(s)
      Wditee R, Buaboocha T, Kato M, Hibino T, Suzuki S, Nakamura T, Takabe T.
    • Journal Title

      Arch.Biochem.Biophys. 450

      Pages: 113-121

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Root specific expression of Na^+/H^+ antiporter gene from Synechosystis sp. PCC6803 confers salt tolerance of tobacco plant2006

    • Author(s)
      Hossain GS, Waditee R, Hibino T, Tanaka Y, Takabe T.
    • Journal Title

      Plant Biotechnol. 23

      Pages: 275-281

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cloning, functional expression and primary characterization of Vibrio parahaemolyticus K^+/H^+ antiporter genes in Escherichia coli.2006

    • Author(s)
      Radchenko MV, Waditee R, Oshimi S, Fukuhara M, Takabe T, Nakamura T.
    • Journal Title

      Mol.Microbiol. 59

      Pages: 651-663

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Conformational changes during apoplastocyanin folding observed by photocleavable modification and transient grating2006

    • Author(s)
      Hirota S, Fujimoto Y, Choi J, Baden N, Katagiri N, Akiyama M, Hulsker R, Ubbink M, Okajima T, Takabe T, Funasaki N, Watanabe Y, Terazima M.
    • Journal Title

      J.Am.Chem.Soc. 128

      Pages: 7551-7558

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Reduction of plastocyanin by tyrosine-containing oligopeptides2006

    • Author(s)
      Hirota S, Okumura H, Kondoh T, Funasaki N, Takabe T, Watanabe Y.
    • Journal Title

      J.Inorg.Biochem. 100

      Pages: 1871-1878

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis2005

    • Author(s)
      Waditee R, Bhuiyan MN, Rai V, Aoki K, Tanaka Y, Hibino T, Suzuki S, Takano J, Jagendorf AT, Takabe T, Takabe T
    • Journal Title

      Proc. Natl. Acad. Sci. USA 102

      Pages: 1318-1323

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains NapA-type Na^+/H^+ antiporters with novel ion specificity that are involved in salt tolerance at alkaline pH2005

    • Author(s)
      Wutipraditkul, N., Waditee, R., Incharoensakdi, A., Hibino, T., Tanaka, Y., Nakamura, T., Shikata, M., Takabe, T., Takabe, T.
    • Journal Title

      Appl. Environ. Microbiol. 71

      Pages: 4176-4184

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Functional differences between cyanobacterial DnaK1 chaperones from the halophyte Aphanothece halophytica and the freshwater species Synechococcus elongatus expressed in Escherichia coli2005

    • Author(s)
      Blanco-Rivero MC, Takabe T, Viale AM
    • Journal Title

      Curr. Microbiol. 51

      Pages: 164-170

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Suppressed expression of apoplastic ascorbate oxidase gene increases salt tolerance in tobacco and Arabidopsis plants2005

    • Author(s)
      Yamamoto, A., Bhuiyan, M.N., Waditee, R., Tanaka, Y., Esaka, M., Oba, K., Jagendorf, A.T., Takabe, T.
    • Journal Title

      J. Exp. Bot. 56

      Pages: 1785-1796

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] All four putative selectivity filter glycine residues in KtrB are essential for high affinity and selective K+ uptake by the KtrAB system from Vibrio alginolyticus2005

    • Author(s)
      Tholema N, Vor der Bruggen M, Maser P, Nakamura T, Schroeder JI, Kobayashi H, Uozumi N, Bakker EP
    • Journal Title

      J. Biol. Chem. 280

      Pages: 41146-41154

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis2005

    • Author(s)
      Waditee R, Bhuiyan MN, Rai V, Aoki K, Tanaka Y, Hibino T, Suzuki S, Takano J, Jagendorf AT, Takabe T, Takabe T.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 102

      Pages: 1318-1323

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains NapA-type Na^+/H^+ antiporters with novel ion specificity that are involved in salt tolerance at alkaline pH2005

    • Author(s)
      Wutipraditkul, N., Waditee, R., Incharoensakdi, A., Hibino, T., Tanaka, Y, Nakamura, T., Shikata, M., Takabe, T., Takabe, T.
    • Journal Title

      Appl.Environ.Microbiol. 71

      Pages: 4176-4184

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Functional differences between cyanobacterial DnaK1 chaperones from the halophyte Aphanothece halophytica and the freshwater species Synechococcus elongatus expressed in Escherichia coli2005

    • Author(s)
      Blanco-Rivero MC, Takabe T, Viale AM.
    • Journal Title

      Curr.Microbiol. 51

      Pages: 164-170

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Suppressed expression of apoplastic ascorbate oxidase gene increases salt tolerance in tobacco and Arabidopsis plants2005

    • Author(s)
      Yamamoto, A., Bhuiyan, M.N., Waditee, R., Tanaka, Y., Esaka, M., Oba, K., Jagendorf, A.T., Takabe, T.
    • Journal Title

      J.Exp.Bot. 56

      Pages: 1785-1796

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] All four putative selectivity filter glycine residues in KtrB are essential for high affinity and selective K+ uptake by the KtrAB system from Vibrio alginolyticus2005

    • Author(s)
      Tholema N, Vor der Bruggen M, Maser P, Nakamura T, Schroeder JI, Kobayashi H, Uozumi N, Bakker EP.
    • Journal Title

      J.Biol.Chem. 280

      Pages: 41146-41154

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Isolation and functional characterization of Ca^<2+>/H^+ antiporters from cyanobacteria2004

    • Author(s)
      Waditee R, Hossain GS, Tanaka Y, Nakamura T, Shikata M, Takano J, Takabe T, Takabe T
    • Journal Title

      J. Biol. Chem. 279

      Pages: 2213-2216

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Interaction of plastocyanin with oligopeptides : effect of lysine distribution within the peptide2004

    • Author(s)
      Hirota S, Okumura H, Arie S, Tanaka K, Shionoya M, Takabe T, Funasaki N, Watanabe Y
    • Journal Title

      J. Inorg. Biochem. 98

      Pages: 849-856

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Isolation and functional characterization of Ca^<2+>/H^+ antiporters from cyanobacteria2004

    • Author(s)
      Waditee R, Hossain GS, Tanaka Y, Nakamura T, Shikata M, Takano J, Takabe T, Takabe T.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 2213-2218

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Interaction of plastocyanin with oligopeptides : effect of lysine distribution within the peptide2004

    • Author(s)
      Hirota S, Okumura H, Arie S, Tanaka K, Shionoya M, Takabe T, Funasaki N, Watanabe Y.
    • Journal Title

      J.Inorg.Biochem. 98

      Pages: 849-855

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Abiotic stress tolerance in plants2006

    • Author(s)
      Rai AK, Takabe T
    • Total Pages
      1-267
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Metabolic engineering of glycinebetaine.2006

    • Author(s)
      Takabe T, Rai V, Hibino T
    • Total Pages
      137-151
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Metabolic engineering of glycinebetaine.2006

    • Author(s)
      Waditee R, Tanaka Y, Takabe T
    • Total Pages
      137-151
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Abiotic stress tolerance in plants2006

    • Author(s)
      Rai AK, Takabe T. (book eds)
    • Total Pages
      1-267
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Metabolic engineering of glycinebetaine. In Abiotic stress tolerance in plants2006

    • Author(s)
      Takabe T, Rai V, Hibino T.
    • Total Pages
      137-151
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Na^+/H^+ antiporters in plants and cyanobacteria. In Abiotic stress tolerance in plants2006

    • Author(s)
      Waditee R, Tanaka Y, Takabe T.
    • Total Pages
      163-175
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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