2006 Fiscal Year Final Research Report Summary
Molecular mechanisms of synthesis, transport, and accumulation of glycinebetaine in plants and cyanobacteria
| Project/Area Number |
16370028
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Meijo University |
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Principal Investigator |
TAKABE Teruhiro Meijo University, Research Institute, Professor, 総合研究所, 教授 (80097766)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Yoshito Meijo University, Faculty of Science & Technology, Associate Professor, 理工学部, 助教授 (10247679)
HIBINO Takashi Meijo University, Faculty of Science & Technology, Associate Professor, 理工学部, 助教授 (70218741)
NAKAMURA Tatsunosuke Niigata University, Pharmacy & Applied Life Science, Professor, 薬学部, 教授 (20114308)
SHIKATA Masamitsu Shimadzu Co., Genomic research, Director, 分析計測事業部ジェノミックリサーチ室, 副主任研究員 (20374126)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Grovel environment / Food / Salt stress / Glycinebetaine / Genetic engineering / Plants / Halotolerant cyanobacterium / Transport |
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| Research Abstract |
Glycine betaine (betaine) is a important osmoprotectant which accumulates under abiotic stresses. In this projects, we have studied molecular mechanisms of synthesis, transport and accumulation of betaine in plantas and halotolerant cyanobacteria. In this project, we obtained the following results. 1) Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis. 2) Isolation and functional characterization of Ca^<2+>/H^+ antiporters from cyanobacteria. 3) Isolation and functional characterization of betaine transporters from halotolerant cyanobacterium and mangrove. 4) Cloning, functional expression and primary characterization of Vibrio parahaemolyticus K^+/H^+ antiporter Escherichia coli ChaA. 4) Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity. 5) Carboxyl-terminal hydrophilic tail of a NhaP type Na^+/H^+ antiporter from cyanobacteria is involved in the apparent affinity for Na^+ and pH sensitivity. 6) Root specific expression of Na^+/H^+ antiporter gene from Synechosystis sp. PCC6803 confers salt tolerance of tobacco plant.
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[Journal Article] Conformational changes during apoplastocyanin folding observed by photocleavable modification and transient grating.2006
Author(s)
Hirota S, Fujimoto Y, Choi J, Baden N, Katagiri N, Akiyama M, Hulsker R, Ubbink M, Okajima T, Takabe T, Funasaki N, Watanabe Y, Terazima M
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Journal Title
J. Am. Chem. Soc. 128
Pages: 7551-7558
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Conformational changes during apoplastocyanin folding observed by photocleavable modification and transient grating2006
Author(s)
Hirota S, Fujimoto Y, Choi J, Baden N, Katagiri N, Akiyama M, Hulsker R, Ubbink M, Okajima T, Takabe T, Funasaki N, Watanabe Y, Terazima M.
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Journal Title
J.Am.Chem.Soc. 128
Pages: 7551-7558
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis2005
Author(s)
Waditee R, Bhuiyan MN, Rai V, Aoki K, Tanaka Y, Hibino T, Suzuki S, Takano J, Jagendorf AT, Takabe T, Takabe T
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Journal Title
Proc. Natl. Acad. Sci. USA 102
Pages: 1318-1323
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains NapA-type Na^+/H^+ antiporters with novel ion specificity that are involved in salt tolerance at alkaline pH2005
Author(s)
Wutipraditkul, N., Waditee, R., Incharoensakdi, A., Hibino, T., Tanaka, Y., Nakamura, T., Shikata, M., Takabe, T., Takabe, T.
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Journal Title
Appl. Environ. Microbiol. 71
Pages: 4176-4184
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Suppressed expression of apoplastic ascorbate oxidase gene increases salt tolerance in tobacco and Arabidopsis plants2005
Author(s)
Yamamoto, A., Bhuiyan, M.N., Waditee, R., Tanaka, Y., Esaka, M., Oba, K., Jagendorf, A.T., Takabe, T.
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Journal Title
J. Exp. Bot. 56
Pages: 1785-1796
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis2005
Author(s)
Waditee R, Bhuiyan MN, Rai V, Aoki K, Tanaka Y, Hibino T, Suzuki S, Takano J, Jagendorf AT, Takabe T, Takabe T.
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Journal Title
Proc.Natl.Acad.Sci.USA 102
Pages: 1318-1323
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains NapA-type Na^+/H^+ antiporters with novel ion specificity that are involved in salt tolerance at alkaline pH2005
Author(s)
Wutipraditkul, N., Waditee, R., Incharoensakdi, A., Hibino, T., Tanaka, Y, Nakamura, T., Shikata, M., Takabe, T., Takabe, T.
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Journal Title
Appl.Environ.Microbiol. 71
Pages: 4176-4184
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Suppressed expression of apoplastic ascorbate oxidase gene increases salt tolerance in tobacco and Arabidopsis plants2005
Author(s)
Yamamoto, A., Bhuiyan, M.N., Waditee, R., Tanaka, Y., Esaka, M., Oba, K., Jagendorf, A.T., Takabe, T.
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Journal Title
J.Exp.Bot. 56
Pages: 1785-1796
Description
「研究成果報告書概要(欧文)」より
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