• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Final Research Report Summary

Molecular dissection of epigenetic transcriptional regulation of flower homeotic gene.

Research Project

Project/Area Number 16370029
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionNotional Institute of Agrobiological Sciences

Principal Investigator

TAKATSUJI Hiroshi  National Institute of Agrobiological Sciences, Plant Physiology Department, Developmental Biology Lab., Head, 生理機能研究グループ形態発生研究チーム, チーム長 (10360455)

Co-Investigator(Kenkyū-buntansha) TSUCHIMOTO Suguru  Tokyo University, Institute of Molecular and Cellular Biosciences, Assistant professor, 分子細胞生物学研究所, 助手 (60212057)
Project Period (FY) 2004 – 2005
Keywordshomeotic gene / epigenetic regulation / DNA methylation / siRNA / ectopic expression / gene silencing / curly leaf / MADS-box gene
Research Abstract

pMADS3 is a class C floral homeotic gene of petunia specifically expressed in stamens and carpels. Introduction of pMADS3 partial genomic sequence resulted in ectopic expression of endogenous pMADS3 (ect-pMADS3 phenotype) in some transgenic petunia lines, a novel epigenetic event. Inclusion of intron 2 (4 kb) in the transgene was essential to induce ect-pMADS3 phenotype. Some T1 individuals inherited the phenotype even after losing the transgene by segregation. We detected aberrant RNAs encoding sense- and antisense sequences of the intron II. These results suggested the involvement of RNA-directed DNA Methylation (RdDM) in the ect-pMADS3 phenomenon. To examine this possibility, we expressed an inverted repeat (IR) form of 1-kb sub-region of pMADS3 intron 2 (region II) and found that it induces ect-pMADS3 phenotype. We further dissected the region II by this strategy and found that a 300-bp sub-fragment was sufficient to induce the ect-pMADS3 expression. We also found that the DNA sequence in region II was methylated in the ect-pMADS3 lines, indicating a correlation between the DNA methylation and the pMADS3 ectopic expression. Therefore, it was strongly suggested that DNA methylation can lead to transcriptional activation。

  • Research Products

    (6 results)

All 2006 2005 2004 Other

All Journal Article (6 results)

  • [Journal Article] Silencing of an anther-specific zinc-finger gene, MEZ1, causes aberrant meiosis and pollen abortion in petunia.2006

    • Author(s)
      Kapoor S, Takatsuji H
    • Journal Title

      Plant Mol. Biol. in press

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transgene-triggered, epigenetically regulated ectopic expression of a flower homeotic gene pMADS3 in Petunia.2005

    • Author(s)
      Kapoor M et al.
    • Journal Title

      Plant J. 43

      Pages: 649-661

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms。2005

    • Author(s)
      Nakagawa H et al.
    • Journal Title

      Plant J. 41

      Pages: 521-523

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms.2005

    • Author(s)
      Nakagawa H et al.
    • Journal Title

      Plant J. 41

      Pages: 512-523

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The Petunia ortholog of Arabidopsis SUPERMAN plays a distinct role in floral organ morphogenesis2004

    • Author(s)
      Nakagawa H et al.
    • Journal Title

      Plant Cell 16

      Pages: 920-932

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Silencing of an anther-specific zinc-finger gene, MEZ1, causes aberrant meiosis and pollen abortion in petunia.

    • Author(s)
      Kapoor S, Takatsuji H
    • Journal Title

      Plant Mol.Biol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi