2007 Fiscal Year Final Research Report Summary
Search and analysis of ion channels that regulate flagellar motility
| Project/Area Number |
16370034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YOSHIMURA Kenjiro University of Tsukuba, Grad. Sch. Life Environ. Sci., Professor (10230806)
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| Project Period (FY) |
2004 – 2007
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| Keywords | Flagellar motility / Ion channel / Chlamydomonas / Calcium ion / Mechanoreception / Biophysics / Electrophysiology |
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| Research Abstract |
The bending patterns of cilia and flagella are controlled by the influx of calcium ions through calcium channels that are present in the flagellar membrane. The elevated calcium concentration in the axoplasma alters the bending mode of the cilia and flagella. The flagellar channel in Chlamydomonas was studied in this project.
1. Identification of the ion channels expressed in the flagella
Multiple genes for voltage dependent calcium channel were found in the genome database of Chlamydomonas. The expression of the gene was confirmed by RT-PCR. One gene, CAV2, was found not to be expressed in ppr2 mutant, which has defect in generating flagellar current. An antibody raised against CAV2 immunostained flagella more intensely toward the distal part of the flagellum. We speculate that this distal localization is necessary for avoiding deflagellation, which is also activated by the increased calcium concentration. The expression level of CAV2 increased after deflagellation, which suggests that the gene is regulated as one of the flagella-related genes. Because photophobic response was not observed in a RNA-mediated knockdown strain for the expression of CAV2, CAV2 is probably the flagellar calcium channel required for the backward swimming in the photophobic response.
2. Analysis of TRP channel in the flagella
7 genes for TRP channels were found to be expressed in Chlamydomonas. Antibodies raised against the TRP channels revealed that 4 of them are expressed in the flagella. This is the first observation that motile flagella possess TRP channel.
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[Presentation] MscS homolog of Chlamydomonas functions as a mechanosensitive channel in the intracellular membrane2008
Author(s)
Nakayama, Y. Fujiu, K., Kasai, N., Sokabe, M., Yoshimura, K.
Organizer
Biophysical Society 50th Annual Meeting
Place of Presentation
Long Beach, CA, U.S.A.
Year and Date
20080202-20080206
Description
「研究成果報告書概要(和文)」より
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