Regulation of cell function by protein dephosphorylation

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 16370055
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Miyagi Cancer Center Research Institute (2006); Hokkaido University (2004-2005)
• Principal Investigator: SHIMA Hiroshi Miyagi Cancer Center Research Institute, Division of Cancer Chemotherapy, Head, 薬物療法学部, 部長 (10196462)
• Co-Investigator(Kenkyū-buntansha): TANUMA Nobuhiro Miyagi Cancer Center Research Institute, Division of Cancer Chemotherapy, Staff, 薬物療法学部, 研究員 (40333645) ; NOMURA Miyuki Miyagi Cancer Center Research Institute, Division of Cancer Chemotherapy, Staff, 薬物療法学部, 技官 (40390893) ; KIKUCHI Kunimi Hokkaido University Institute for Genetic Medicine, Divisiojn of Biochemical Oncology and Immunology, Professor, 遺伝子病制御研究所, 教授 (20006117)
• Project Period (FY): 2004 – 2006
• Keywords: TCPTP / MKP7 / LDP4 / PP2A / I-1^<PP2A> / pp32 / I-2^<PP2A> / SET / PP1 / Aurora kinase

Research Abstract

Development of novel inhibitors of PTP
Manzamenone was identified as a novel inhibitor of T-Cell Protein Tyrosine Phosphatase
Regulation of signal transduction by protein phosphatases
(1)Phosphorylation of Ser-446 determined stability of MKP-7.
(2)Low-molecular-mass dual-specificity phosphaase (LDP-3) enhanced activation of JNK and p38.
Regulation of transcription and translation by protein phosphatases
(1)Theoncoprotein I-2^<PP2A>/SET negatively regulated the MEK/ERK pathway and cell proliferation.
(2)pp32/I-1^<PP2A> negatively regulated the Raf/MEK/ERK pathway.
Regulation of cell morphology by protein phosphatases
(1)Isoform specific function of PP1 catalytic subunits was observed by using siRNA.
(2)Vimentin-Ser82 was identified as a memory phosphorylation site in astrocytes.
Regulation of cell division by protein phosphatases
PP1 regulated Aurora-A activity by dephosphorylatiohn.

Research Products

• [Journal Article] Characterization of novel low-molecular-mass dual specificity phosphatase-4 (LDP-4) expressed in brain (2007)
  • Author(s): Kentaro Takagaki
  • Journal Title: Mol Cellular Biochem 296 Pages: 177-184
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Manzamenones Inhibits T-Cell Protein Tyrosine Phosphatase (2006)
  • Author(s): Yusuke Wakuda
  • Journal Title: Marine Drugs 4 Pages: 9-14
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Vimentin-Ser82 as a memory phosphorylation site in astrocytes (2006)
  • Author(s): Takashi Oguri
  • Journal Title: Genes to Cells 11 Pages: 531-540
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phospho-regulation of human protein kinae A Aurora-A : analysis using anti-phospho-Thr 288 monoclonal antibodies (2006)
  • Author(s): Sayaka Ohashi
  • Journal Title: Oncogene 25 Pages: 7691-7702
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Vimentin-Ser82 as a memory phosphorylation site in astrocytes. (2006)
  • Author(s): Oguri T, Inoko A, Shima H, Izawa I, Arimura N, Yamaguchi T, Inagaki N, Kaibuchi K, Kikuchi K, Inagaki M.
  • Journal Title: Genes Cells 11 Pages: 531-540
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Manzamenones inhibit T-Cell Protein Tyrosine Phosphatase (2006)
  • Author(s): Wakuda Y, Kubota T, Shima H, Okada T, Mitsuhashi S, Aoki N, Kikuchi K, Kobayashi J.
  • Journal Title: Mar.Drugs 4 Pages: 9-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of a novel low-molecular- mass dual specificity phosphatase-4(LDP-4) expressed in brain (2006)
  • Author(s): Takagaki K, Shima H, Tanuma N, Nomura M, Satoh T, Watanabe M, Kikuchi K.
  • Journal Title: Mol Cell Biochem 296 Pages: 177-184
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The oncoprotein I-2PP2A/SET negatively regulates the MEK/ERK pathway and cell proliferation. (2005)
  • Author(s): Chikako Fukukawa
  • Journal Title: Int J Oncol 26 Pages: 751-756
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Protein phosphatase type 2A, PP2A, is involved in degradation of gp 130. (2005)
  • Author(s): Shinya Mitsuhashi
  • Journal Title: Mol Cell Biochem 269 Pages: 183-187
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] pp32/I-1 PP2A negatively regulates the Raf/MEK/ERK pathway. (2005)
  • Author(s): Chikako Fukukawa
  • Journal Title: Cancer Lett 226 Pages: 155-160
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phosphorylation of Ser-446 determines stability of MKP-7. (2005)
  • Author(s): Chiaki Katagiri
  • Journal Title: J Biol Chem 280 Pages: 14716-14722
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] pp32/I-1^<PP2A> negatively regulates the Raf/MEK/ERK pathway. (2005)
  • Author(s): Fukukawa C, Tanuma N, Okada T, Kikuchi K, Shima H.
  • Journal Title: Cancer Lett 226 Pages: 155-160
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phosphorylation of Ser-446 determines stability of MKP-7. (2005)
  • Author(s): Katagiri C, Masuda K, Urano T, Yamashita K, Kikuchi K, Shima H.
  • Journal Title: J Biol Chem 280 Pages: 14716-14722
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phospho-regulation of human protein kinase Aurora-A : analysis using anti-phopho-Thr288 monoclonal antibodies. (2005)
  • Author(s): Ohashi S, Sakashita G, Ban R, Nagasawa M, Matsuzaki H, Murata Y, Taniguchi H, Shima H, Furukawa K, Urano T.
  • Journal Title: Oncogene 25 Pages: 7691-7702
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of a novel low-molecular-mass dual-specificity phosphaase 3(LDP-3) that enhances activation of JNK and p38. (2004)
  • Author(s): Kentaro Takagaki
  • Journal Title: Biochem J 383 Pages: 447-455
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Analysis of isoform specific function of PP1 catalytic subunits in mammalian cells using siRNA. (2004)
  • Author(s): Tadashi Okada
  • Journal Title: Int J Oncol 25 Pages: 1383-1388
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Nagelamides A-H, new dimeric bromopyrrole alkaloids from marine sponge agelas species. (2004)
  • Author(s): Tadashi Endo
  • Journal Title: J Nat Prod 68 Pages: 1262-1267
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of a novel low-molecular-mass dual-specificity phosphaase 3(LDP-3) that enhances activation of JNK and p38. (2004)
  • Author(s): Takagaki K, Satoh T, Tanuma N, Masuda K, Takekawa M, Shima H, Kikuchi K.
  • Journal Title: Biochem J 383 Pages: 447-455
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Analysis of isoform specific function of PP1 catalytic subunits in mammalian cells using siRNA. (2004)
  • Author(s): Okada T, Fujii T, Tanuma N, Mitsuhashi S, Urano T, Araki Y, Shima H, Kikuchi K.
  • Journal Title: Int J Oncol 25 Pages: 1383-1388
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nagelamides A-H, new dimeric bromopyrrole alkaloids from marine sponge agelas species. (2004)
  • Author(s): Endo T, Tsuda M, Okada T, Mitsuhashi S, Shima H, Kikuchi K, Mikami Y, Fromont J, Kobayashi J.
  • Journal Title: J Nat Prod 68 Pages: 1262-1267
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The oncoprotein I-2^<PP2A>/SET negatively regulates the MEK/ERK pathway and cell proliferation. (2004)
  • Author(s): Fukukawa C, Shima H, Tanuma N, Okada T, Kato N, Adachi Y, Kikuchi K.
  • Journal Title: Int J Oncol 26 Pages: 751-756
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Protein phosphatase type 2A, PP2A, is involved in degradation of gp130. (2004)
  • Author(s): Mitsuhashi S, Shima H, Tanuma N, Sasa S, Onoe K, Ubukata M, Kikuchi K.
  • Journal Title: Mol Cell Biochem 269 Pages: 183-187
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 生化学 (2005)
  • Author(s): 島礼
  • Total Pages: 8
  • Publisher: 生化学会
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 細胞工学 (2003)
  • Author(s): 島礼
  • Total Pages: 4
  • Publisher: 秀潤社
  • Description: 「研究成果報告書概要(和文)」より