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2006 Fiscal Year Final Research Report Summary

Molecular characterization of the construction and function of the membrane microdomain(raft)

Research Project

Project/Area Number 16370062
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionKobe University

Principal Investigator

MAEKAWA Shohei  Kobe-University, Graduate School of Science and Technology, Professor, 自然科学研究科, 教授 (40173695)

Project Period (FY) 2004 – 2006
Keywordsraft / NAP-22 / cholesterol / neuron / bioinformation
Research Abstract

1. Molecular characterization of brain-derived membrane microdomain was performed. The raft-localization of Na/K-ARPase, one of the main ATPase in the cell membrane, was found. The isoform-specific localization change of this ATPase through ouabain-treatment was discovered.
2. NAP-22 binding proteins were detected through immunoprecipitation, and these proteins were identified as protein N-myristoyl transferase-1, and-2. This result suggests the possibility of protein modification on raft.
3. Oligomer-formation of NAP-22 through the maturation of neurons was found. Since cholesterol depletion abolished this formation, membrane cholesterol is found to have an important role in this oligomerization.
4. Neurocalcin a is a raft-localized protein. Alsin, one of the proteins related to ALS(amyotrophic lateral screlosis), was identified as a neurocalcin a binding protein. The molecular interaction of these proteins are under investigation.
5. Raft-localization of phospholipaseC β1, β2, and β3 were found. Purification and molecular characterization of PLC β1 from raft was performed and an inhibitory effect of NAP-22 on the enzymatic activity was found. This result suggests the participation of NAP-22 on the lipid dynamics on the membrane.

  • Research Products

    (6 results)

All 2007

All Journal Article (6 results)

  • [Journal Article] Ouabain-induced isoform-specific localization change of the Na^+, K^+ -ATPase α subunit in the synaptic plasma membrane of rat brain.2007

    • Author(s)
      田口 勝敏
    • Journal Title

      Neurosci. Lett 413

      Pages: 42-45

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Localization of phospholipase Cβ1 on the detergent-resistant membrane microdomain prepared from the synaptic plasma membrane of rat brain.2007

    • Author(s)
      田口 勝敏
    • Journal Title

      J. Neurosci. Res (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Myelin protein zero is one of the components of the detergent-resistant membrane microdomain fraction prepared from rat pituitary2007

    • Author(s)
      田口 勝敏
    • Journal Title

      J. Molecular Histology (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ouabain-induced isoform-specific localization change of the Na+, K+-ATPase a subunit in the synaptic plasma membrane of rat brain2007

    • Author(s)
      Taguchi, K., Kumanogoh, H., Nakamura, S., Maekawa, S.
    • Journal Title

      Neurosci. Lett. 413

      Pages: 42-45

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Localization of phospholipase C β 1 on the detergent-resistan membrane microdomain prepared from the synaptic plasma membrane of rat brain.2007

    • Author(s)
      Taguchi, K., Kumanogoh, H., Nakamura, S., Maekawa, S.
    • Journal Title

      J. Neurosci. Res. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Myelin protein zero is one of the components detergent-resistant membrane microdomain fraction prepared from rat pituitary.2007

    • Author(s)
      Taguchi, K., Kumanogoh, H., Nakamura, S., Miyata, S., Maekawa, S.
    • Journal Title

      J. Mol. Histol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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