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2006 Fiscal Year Final Research Report Summary

Clarification of energy transduction of bacterial flagellar motor using hybrid bacteria

Research Project

Project/Area Number 16370073
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionTOHOKU UNIVERSITY (2006)
Nagoya University (2004-2005)

Principal Investigator

ISHIJIMA Akihiko  Tohoku University, Institute of Multidisciplinary Research for Advanced Materials, Professor, 多元物質科学研究所, 教授 (80301216)

Project Period (FY) 2004 – 2006
Keywordsbacteria / nano measurement / moter / single molecule measurement / ion
Research Abstract

Bacterial flagellar motor is known as a molecular machine of bacteria, of which diameter is about 40 nm. The driving force is a difference of ion concentration, proton and sodium, between the inside and outside of the cell. We have made the chimera cell using these cells. This cell is suitable for a rotational measurement, plays an important role for elucidation of this mechanism. We succeeded in a measurement of step-size motion of rotation for the first time (Nature, 2005). However, it was not yet confirmed about a basic property of this chimera cell. Therefore we tried to clarify a basic property of this chimera cell. As a result, the torque-speed relations of a chimera cell had the similar characteristics of two motors. Furthermore, stepwise increase of the rotational speed with an incorporation of stator complex was observed, and the torque-speed relationship by one stator complex could be measured.
In addition, imaging technology in a cell is necessary to clarify the information in bacteria cell, the construction situation of a motor complex and a rotational motion a motor. There are several problems about the imaging technology using bacteria cell compared to the normal method, by small and shape of cells. We optimized evanescent illumination and tried to observe of GFP fused protein in bacteria cell. As a result, we succeeded in observing the diffusional motion of GFP fused FliG protein which is one of the motor proteins. Furthermore, we succeeded in estimating the diffusion constant of the fluorescent spots. In addition, we were able to make clear that the position of the GFP fused FliG protein coincided with a pivot point of a cell using a tethered cell assay method.

  • Research Products

    (13 results)

All 2007 2006

All Journal Article (10 results) Book (3 results)

  • [Journal Article] Visualization of functional rotor proteins of the bacterial flagellar motor in the cell membrane.2007

    • Author(s)
      Fukuoka, H.
    • Journal Title

      J Mol Biol 367

      Pages: 692-701

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Chara myosin and the energy of cytoplasmic streaming.2006

    • Author(s)
      Yamamoto K
    • Journal Title

      Plant Cell Physiol. 47

      Pages: 1427-1431

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 現場で役立っバイオイメージング・光ピンセット 基礎編2006

    • Author(s)
      石島秋彦
    • Journal Title

      バイオニクス 8月号

      Pages: 70-75

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 現場で役立つバイオイメージング・光ピンセット 応用編2006

    • Author(s)
      石島秋彦
    • Journal Title

      バイオニクス 9月号

      Pages: 68-72

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 映像化された二次元液晶膜小胞の動的性質2006

    • Author(s)
      稲葉岳彦
    • Journal Title

      液晶 Vol. 10. No. 3

      Pages: 48-56

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Chara myosin and the energy of cytoplasmic streaming.2006

    • Author(s)
      Yamamoto, K.
    • Journal Title

      Plant Cell Physiol. 47

      Pages: 1427-1431

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Basic crochet of Bioimaging light tweezers useful on the site2006

    • Author(s)
      Ishijima, A.
    • Journal Title

      Bionics, August issue

      Pages: 70-75

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Application of Bioimaging light tweezers useful on the site2006

    • Author(s)
      Ishijima, A.
    • Journal Title

      Bionics, September issue

      Pages: 68-72

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A dynamic property of a picturized two-dimensional liquid crystal membrane vesicles2006

    • Author(s)
      Inaba, T.
    • Journal Title

      Liquid crystal Vol.10.No.3

      Pages: 48-56

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A step measurement and exercise analysis of a creature molecule motor.2006

    • Author(s)
      Nishiyama, M.
    • Journal Title

      Properties of matter study

      Pages: 29

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 生物分子モーターのステップ計測と運動解析2006

    • Author(s)
      西山雅祥
    • Total Pages
      29
    • Publisher
      物性研究
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 科学立国日本を築く 極限に挑む気悦の研究者たち2006

    • Author(s)
      石島秋彦
    • Total Pages
      9
    • Publisher
      日刊工業新聞社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Establish Japan as a nation based on the creativity of science.2006

    • Author(s)
      Ishijima, A.
    • Total Pages
      9
    • Publisher
      A daily publication industry newspaper publishing company
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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