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2007 Fiscal Year Final Research Report Summary

Analysis of mRNA localization mechanism in neuronal cells mediated by splicing-dependent complex, EJC

Research Project

Project/Area Number 16370078
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionTokyo Medical and Dental University (2007)
Kyoto University (2004-2006)

Principal Investigator

KATAOKA Naoyuki  Tokyo Medical and Dental University, Medical Research Institute, Guest Lecturer (60346062)

Project Period (FY) 2004 – 2007
Keywordssplicing / Exon Junction Complex / Cap structure / neuronal cells / Nuclear Cap binding Complex / Y14 / cytoplasmic localization / local translation
Research Abstract

It has been well accepted that steps of gene expression influence to each other in higher eukaryotes. For example, splicing not only removes intron from pre-mRNAs but also deposits exon junction complex near exon-exon junction of mRNA EJC enhances downstream events, such as translation, transport and nonsense mediated mRNA decay (NMD). Y14 and Magoh, core components of EJC, bind to mRNA in the nucleus and remain bound in the cytoplasm. This has strongly suggested that these proteins have roles in RNA localization NMD. Indeed, homologs of these proteins in Drosophila have been shown to be involved in oskar mRNA localization in oocytes. In addition, others and we also showed that these proteins participate in NMD.
In this research, we hypothesized that EJC also links mRNA splicing with mRNA localization in mammalian cells, and have been investigating the molecular mechanism of mRNA localization in neuronal cells. We have identified by immunostaining that there are granules that contain nuclear cap binding complex (CBC) and Y14 in the shaft regions of neuritis. These granules are associated with microtubules and thought to be transported along them. We also found granules that contain cytoplasmic cap binding protein, eIF4E, in the branched regions of neuronal cells. By performing immunostaining, we demonstrated that both granules contain Dcp1a, which is a marker of P body. These results indicate that there are two different p body-like granules in neuronal cells; one is CBC-EJC containing transportable p body, the other is eIF4E containing anchored p body. Our findings strongly suggest a new regulatory mechanism of local translation for localized mRNAs in neurons.

  • Research Products

    (6 results)

All 2008 Other

All Journal Article (4 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Preparation of efficient splicing extracts from whole cells, nuclei, and cytoplasmic fractions.2008

    • Author(s)
      Kataoka, N
    • Journal Title

      Methods in Molecular Biology 488

      Pages: 357-365

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Preparation of efficient splicing extracts from whole cells, nuclei, and cytoplasmic fractions.2008

    • Author(s)
      Kataoka, N., Dreyfuss, G.
    • Journal Title

      RNA-Protein Interaction Protocols, 2nd edition. (Editor: Ren-Jang Lin) Methods in Molecular Biology 488

      Pages: 357-365

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Isolation and characterization of post-splicing lariat-intron complexes

    • Author(s)
      Yoshimoto, R.
    • Journal Title

      Nucleic Acids Research (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of post-splicing lariat-intron complexes

    • Author(s)
      Yoshimoto, R., Kataoka, N^*., Okawa, K., Ohno, M. ^* (^*Corresponding authors)
    • Journal Title

      Nucleic Acids Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Transition from transport to localization by mRNP remodeling in neurons2008

    • Author(s)
      Kataoka, N.
    • Organizer
      10^<th> Annual meeting of Japanese RNA society
    • Place of Presentation
      Sapporo Convention Center
    • Year and Date
      20080723-20080725
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 神経突起中におけるmRNPのリモデリングと輸送から局在への移行2008

    • Author(s)
      片岡直行
    • Organizer
      第10回日本RNA学会年会(第10回RNAミーティング)
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2008-07-24
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-06-09  

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