Evolution of the sex determining genes in the medaka fishes

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 16370094
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Niigata University
• Principal Investigator: SAKAIZUMI Mitsuru Niigata University, Institute of Science and Technology, Professor, 自然科学系, 教授 (40175360)
• Co-Investigator(Kenkyū-buntansha): HAMAGUCHI Satoshi Niigata University, Institute of Science and Technology, Professor, 自然科学系, 教授 (20126444) ; NARUSE Kiyoshi Niigata University, Department of Biological Sciences, Graduate School of Science, Lecturer, 理学系研究科, 講師 (50208089)
• Project Period (FY): 2004 – 2006
• Keywords: SEX DETERMINATION / SEX CHROMOSOME / MEDAKA FISH / EVOLUTION / SEX DETERMINING GENE

Research Abstract

1. Sex determination system and the sex chromosomes of six medaka-related species were identified: Oryzias luzonensis, XX-XY system and linkage group (LG)12 of the medaka O.latipes ; O.mekongensis, XX-XY and LG2 ; O.dancena, XX-XY and LG10 ; O.hubbsi, ZZ-ZW and LG5 ; O.javanicus, ZZ-ZW and L G16 ; O.minutillus, XX-XY and LG8.
2. A BAC library derived from XX cells of O luzonensis was constructed. Four BAC clones covered the sex-determining (SD) region of the X chromosome in this species. The SD region was 350 kb in length. The complete nucleotide sequence of this region revealed overall synteny to the corresponding region of the medaka LG12.
3. The SD region of O.dancena was mapped on a scaffold of medaka LG10. This region corresponds to 1.4 Mb in the medaka. Two BAC libraries derived from each of XX and YY cells were constructed.
4. A fluorescent in situ hybridization analysis demonstrated that O.hubbsi had heteromorphic sex chromosomes with characteristic telomeric heterochromatin blocks The sex chromosome had a telomeric inversion between the X and Y. The SD region was mapped in the inversion.

Research Products

• [Journal Article] Evolution of ZZ/ZW and XX/XY sex-determination systems in the closely related medaka species, Oryzias hubbsi and O. dancena (2007)
  • Author(s): Takehana, Y
  • Journal Title: Chromosoma (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evolution of different Y chromosomes in two medaka species, Oryzias dancena and O. latipes (2007)
  • Author(s): Takehana, Y
  • Journal Title: Genetics 175 Pages: 1335-1340
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evolution of different Y chromosomes in two medaka species, Oryzias dancena and 0. latipes. (2007)
  • Author(s): Takehana, Y., Demiyah, D., Naruse, K., Hamaguchi, S., Sakaizumi, M.
  • Journal Title: Genetics 175 Pages: 1335-1340
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Wild-derived XY sex-reversal mutants in the medaka, Oryzias latipes. (2006)
  • Author(s): Otake, H
  • Journal Title: Genetics 173 Pages: 2083-2090
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Interspecific hybridization between Oryzias latipes and Oryzias curvinotus causes XY sex reversal (2006)
  • Author(s): Shinomiya, A
  • Journal Title: J. Exp. Zool 305A Pages: 890-896
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Interspecific hybridization between Oryzias latipes and Oryzias curvinotus causes XY sex reversal. (2006)
  • Author(s): Shinomiya, A., Kato M., Yaezawa, M., Sakaizumi, M., Hamaguchi, S.
  • Journal Title: J.Exp.Zool. 305A Pages: 890-896
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular phylogeny of the medaka fishes genus Oryzias (Beloniformes : Adrianichthyidae) based on nuclear and mitochondrial DNA sequences (2005)
  • Author(s): Takehana Y
  • Journal Title: Molecular Phylogenetics and Evolution 36 Pages: 417-428
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular phylogeny of the medaka fishes genus Oryzias (Beloniformes : Adrianichthyidae) based on nuclear and mitochondrial DNA sequences. (2005)
  • Author(s): Takehana, Y., Naruse, K., Sakaizumi, M.
  • Journal Title: Phylogenetics and Evolution 36 Pages: 417-428
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The XX-XY sex-determination system in Oryzias luzonensis and O. mekongensis revealed by the sex ratio of the progeny of sex-reversed fish (2004)
  • Author(s): Hamaguchi S.
  • Journal Title: Zoological Science 21 Pages: 1015-1018
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The XX-XY sex-determination system in Oryzias luzonensis and 0. mekongensis revealed by the sex ratio of the progeny of sex-reversed fish. (2004)
  • Author(s): Hamaguchi, S., Toyazaki, Y., Shinomiya, A., Sakaizumi, M.
  • Journal Title: Zool.Sci. 21(10) Pages: 1015-1018
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evolution of ZZ/ZW and XX/XY sex-determination systems in the closely related medaka species, Oryzias hubbsi and 0. dancena.
  • Author(s): Takehana, D., Naruse, K., Hamaguchi, S., Sakaizumi, M.
  • Journal Title: Chromosoma (in press)
  • Description: 「研究成果報告書概要(欧文)」より