2006 Fiscal Year Final Research Report Summary
Analyses of molecular genetic mechanism involved in the sporophytic self-incompatibility of Ipomoea.
| Project/Area Number |
16380005
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Breeding science
|
|---|
| Research Institution | Mie University |
|---|
Principal Investigator |
KOWYAMA Yasuo Mie University, Graduate School of Bioresources, Professor, 大学院生物資源学研究科, 教授 (80024579)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAKEDA Katsuyuki Mie University, Graduate School of Bioresources, Associate Professor, 大学院生物資源学研究科, 助教授 (50221867)
|
|---|
| Project Period (FY) |
2004 – 2006
|
| Keywords | Self-incompatibility / Convolvulaceae / Ipomoea / Sweet potato relatives / Sexual reproduction / Molecular genetics / Genome analysis |
|---|
| Research Abstract |
Self-incompatibility (SI) is a genetic system that prevents self-fertilization and thereby promotes out-crossing to maintain genetic diversity in plant populations. The SI system works through the rejection of self-pollen following the recognition of self and non-self pollen by the pistil. In the plant family, Convolvulaceae, several species of Ipomoea possess a sporophytic type of SI that is regulated by a single multiallelic S-locus. The present study was performed to identify the S-locus genes involved in the self-pollen recognition and to reveal molecular mechanism of the SI system in Ipomoea. Research results obtained are summarized as follows. (1) Fine genetic map of DNA markers was constructed in the vicinity of the S-locus. (2) Using a map-based positional cloning strategy, several genomic clones covering the S-locus were screened from bacterial artificial chromosome (BAC) and cosmid libraries of three S haplotypes. (3) Sequence analysis of the isolated clones showed that the S-loci were delimited to the S haplotype-specific divergent regions (SDRs) spanning 30 to 95 kb in size. (4) From gene expression analyses by Northern blot and RT-PCR of mRNAs, three stigma-specific genes (SE1, SE2, SEA) and an anther-specific gene (AB2) in the SDRs were identified as candidates encoding pistil and pollen determinants of the SI, respectively. (5) Transformants introduced with RNAi constructs of the stigma-specific genes showed no changes in the SI phenotypes, indicating that the stigma-specific genes might be functioned as complementary. (6) The present study revealed that these stigma specific candidates showed no homologies with the S-locus genes reported to date from other plants, and suggests that a unique molecular mechanism is involved in the SI system of Ipomoea plants.
|
-
-
-
[Journal Article] Physical size of the S locus region defined by genetic recombination and genome sequencing in Ipomoea trifida, Convolvulaceae.2007
Author(s)
Rahman, M.H., Tsuchiya, T., Suwabe, K., Kohori, J., Tomita, R.N., Kagaya, Y., Kobayashi, I., Kakeda, K., Kowyama, Y.
-
Journal Title
Sexual Plant Reproduction online published
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Expression of stigma-and anther-specific genes located in the S locus region of Ipomoea trifida.2007
Author(s)
Rahman, M.H., Uchiyama, M., Kuno, M., Hirashima, N., Suwabe, K, Tsuchiya, T., Kagaya, Y., Kobayashi, I., Kakeda, K., Kowyama, Y.
-
Journal Title
Sexual Plant Reproduction online published
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Gene expression analyses of the S-locus region confer- ring a sporophytic self-incompatibility in Ipomoea, Convolvulaceae.2006
Author(s)
Kowyama, Y., Rahman, M.H., Suwabe, K., Uchiyama, M., Kuno, M., Kakeda, K., Tsuchiya, T.
-
Journal Title
Abstract of the 19th International Congress on Sexual Plant Reproduction 19
Pages: 166-167
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
[Journal Article] Molecular characterization of a 313-kb genomic region containing the self-incompatibility locus of Ipomoea trifida2004
Author(s)
Tomita, R.N., Suzuki, G, Yoshida, K., Yano, Y., Tsuchiya, T., Kakeda, K., Mukai, Y, Kowyama, Y.
-
Journal Title
Breeding Science 54
Pages: 165-175
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Visualization of the S-locus region in Ipomoea trifida : toward positional cloning of self-incompatibility genes.2004
Author(s)
Suzuki, G, Tanaka, S., Yamamoto, M., Tomita, R.N., Kowyama, Y., Mukai, Y.
-
Journal Title
Chromosome Research 12
Pages: 475-481
Description
「研究成果報告書概要(欧文)」より
-
