2006 Fiscal Year Final Research Report Summary
Regulation of host DNA replication by nucleopolyhedrovirus infection
| Project/Area Number |
16380040
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Nagoya University |
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Principal Investigator |
IKEDA Motoko Nagoya University, Graduate School of Bioagricultural Sciences, Asscciate prefessor, 大学院生命農学研究科, 助教授 (20262892)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Michihiro Nagoya University, Graduate School of Bioagricultural Sciences, Profossor, 大学院生命農学研究科, 教授 (60111837)
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| Project Period (FY) |
2004 – 2006
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| Keywords | nucleopolyhedrovirus / host DNA replication arrest / PCNA / apoptosis |
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| Research Abstract |
A homologue of proliferating cell nuclear antigen (pcna) gene is identified in Autographa californica nucleopolyhedrovirus genome, but the function in viral replication has not been elucidated. In the present study, AcMNPV mutants defective in the pcna gene (vAcApcnas) were generated and the function of the pcna gene was analyzed. Biological characterization revealed that vAcApcnas were viable in the Sf9 cells and the kinetics of viral DNA replication, viral structural protein synthesis, budded virus production and polyhedron production in the vAcApcna-infected cells were comparable to those in the cells infected with wt AcMNPV. It was also demonstrated that the amount of cellular PCNA in the nuclear fraction of Sf9 cells infected with vAcApcna was significantly higher than that of wt AcMNPV-infected cells. Further analysis by DNase I treatment and chromatin immunoprecipitation assay revealed that the cellular PCNA in the nucleus of Sf9 cells bound AcMNPV DNA. The amount of cellular PCNA associated with viral DNA replication sites was greater in cells infected with vAcApcna than in cells infected with wt AcMNPV. These results suggest that both cellular and viral PCNAs are involved in AcMNPV DNA replication and that vAcApcna is able to substitute cellular PCNA for viral PCNA. This deprivation of cellular PCNA may cause host DNA synthesis shut down.
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[Journal Article] Cloning and comparative characterization of nucleopolyhedroviruses isolated from African bollworm, Helicoverpa armigera, (Lepidoptera : Noctuidae) in different geographic regions.2007
Author(s)
Ogembo, J.G., Chaeychomsri, S., Kamiya, K., Ishikawa, H., Katou, Y., Ikeda, M., Kobayashi, M.
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Journal Title
J. Insect Biotechnol Sericol. 76
Pages: 39-49
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] hycu-hr6, a large homologous region of Hyphantria cunea nucleopolyhedrovirus, is dispensable for virus replication in culture cells.2006
Author(s)
Nagai, S., Felipe Alves, C.A., Shirata, N., Katou, Y., Ishikawa, H., Ikeda, M., Kobayashi, M.
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Journal Title
J. Insect Biotechnol. Sericol 75
Pages: 57-64
Description
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[Journal Article] Cloning and comparative characterization of three distinct nucleopolyhedroviruses isolated from the common cutworm, Spodoptera litura (Lepidoptera : Noctuidae) in Japan.2004
Author(s)
Kamiya, K., Zhu, J., Murata, M., Lavina-Caoili, BA., Ikeda, M., Kobayashi, M., Kawamura, S.
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Journal Title
Biol. Cont. 31
Pages: 38-48
Description
「研究成果報告書概要(欧文)」より
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