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2005 Fiscal Year Final Research Report Summary

Studies on the establishment and element supply ability of arbusculer mycorrhizal symbiosis using the hyper-mycorrhizal soybean mutant

Research Project

Project/Area Number 16380046
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionChiba University

Principal Investigator

SAKAMOTO Kazunori  Chiba University, Faculty of Horticulture, Associate Professor, 園芸学部, 助教授 (10225807)

Co-Investigator(Kenkyū-buntansha) TAWARAYA Keitaro  Yamagata University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70179919)
EZAWA Tatsuhiro  The University of Hokkaido, Graduate School of Agriculture, Associate Professor, 大学院・農学研究科, 助教授 (40273213)
ISOI Toshiyuki  Meijo University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (30211733)
SUZUKI Hiroyuki  Chiba University, Graduate School of Pharmaceutical Sciences, Research Associate, 大学院・薬学研究院, 助手 (70302578)
Project Period (FY) 2004 – 2005
KeywordsSoybean / Arbuscular mycorrhizal fungi / Hyper-mycorrhizal mutant / Systemic regulation / reponse for phosphate application / Poly-phosphate metabolism / microelement uptake / Neutron activation analysis
Research Abstract

We studied the establishment and element supply ability of arbusculer mycorrhizal symbiosis using the hyper-mycorrhizal soybean mutant. The results obtained were summarized as follows :
1. Hyper-mycorrhizal soybean mutant, En6500, showed higher arbuscular abundance compared with the wild type Enrei. We elucidated that soybean shoot is responsible for the negative regulation of arbuscule formation.
2. We found that the root-derived material of soybean root is not involved in the growth of external hyphae of arbuscular mycorrhizal fungi.
3. Hyper-nodulating soybean genotype Sakukei 4, as well as En6500, showed higher arbuscular abundance compared with the wild type Enrei.
4. We found the poly-phosphate accumulating organella involved in the transport of phosphate in the external hyphae of arbuscular mycorrhizal fungi.
5. We clarified that the uptake of some microelements are related to the degree of arbuscule formation in the root.

  • Research Products

    (8 results)

All 2006 2005 2004

All Journal Article (8 results)

  • [Journal Article] Solubilization of insoluble inorganic phosphate by hyphal exudates of arbuscular mycorrhizal fungi.2006

    • Author(s)
      Tawaraya, K.
    • Journal Title

      Journal of Plant Nutrition 29

      Pages: 1-9

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] MIDIシステムを用いた脂肪酸組成分析による畑作物根に定着したアーバスキュラー菌根菌の検出2005

    • Author(s)
      坂本 一憲
    • Journal Title

      日本土壌肥料学雑誌 76・3

      Pages: 317-320

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Autoregulation system of soybean controls both rhizobial nodulation and arbuscular mycorrhizal colonization.2005

    • Author(s)
      Sakamoto, K.
    • Journal Title

      Plant nutrition for food security, human health and environmental protection

      Pages: 824-825

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A new hypothesis on the strategy for acquisition of phosphorus in arbuscular mycorrhiza : up-regulation of secreted acid phosphatase gene in the host plant.2005

    • Author(s)
      Ezawa, T.
    • Journal Title

      Molecular Plant-Microbe Interactions 18

      Pages: 1046-1053

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Influence of arbuscular mycorrhizal colonization on the growth of super-nodulating soybean genotype Sakukei 4.2005

    • Author(s)
      Isoi, T.
    • Journal Title

      Plant nutrition for food security, human health and environmental protection

      Pages: 834-835

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Use of fatty acid profiles analyzed by MIDI system for detecting crop-root colonization of arbuscular mycorrhizal fungi.2005

    • Author(s)
      Sakamoto, K.
    • Journal Title

      Japanese Journal of Soil Science and Plant Nutrition (in Japanese) 76(3)

      Pages: 317-320

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Use of specific phospholipid fatty acids for identifying and quantifying the external hyphae of the arbuscular mycorrhizal fungus Gigaspora rosea.2004

    • Author(s)
      Sakamoto, K.
    • Journal Title

      Soil Biology and Biochemistry 36・11

      Pages: 1827-1834

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Use of specific phospholipid fatty acids for identifying and quantifying the external hyphae of the arbuscular mycorrhizal fungus Gigaspora rosea.2004

    • Author(s)
      Sakamoto, K.
    • Journal Title

      Soil Biology and Biochemistry 36(11)

      Pages: 1827-1834

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

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