2005 Fiscal Year Final Research Report Summary
Basic Research on the Development of an Artificial Root Nodule for a Novel Nitrogen Fixation System.
| Project/Area Number |
16380048
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | National University Corporation Tokyo University of Agriculture and Technology |
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Principal Investigator |
YOKOYAMA Tadashi National University Corporation Tokyo University of Agriculture and Technology, Institute of symbiotic science and technology, associate professor, 大学院・共生科学技術研究部, 助教授 (70313286)
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| Co-Investigator(Kenkyū-buntansha) |
ARIMA Yasuhiro National University Corporation Tokyo University of Agriculture and Technology, Institute of symbiotic science and technology, Professor, 大学院・共生科学技術研究部, 教授 (90011973)
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| Project Period (FY) |
2004 – 2005
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| Keywords | soybean / root nodule / Bradyrhizobium / symbiosome / bacteroid / symbiosis / biological nitrogen fixation / rhizobium |
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| Research Abstract |
Bradyrhizobium is a symbiont of soybean. Bradyrhizobium cells differentiate into bacteroids in soybean root nodule cells and start nitrogen fixation. However, a molecular mechanism of the differentiation from free living cell to bacteroid in root nodule cells is stll unknown. The bacteroids enclose with PBS (peri-bacteroidal space)-solution in symbiosome. The PBS-solution expects to have several plant signals involving in deformation processes from rhizobium to bacteroids or maintenance of bacteroids. We compared profiles of gene expressions between B.japonicum USDA 110 with and without the PBS-solution isolated from soybean root nodules in terms of analysis by macroarray containing 3960 DNA fragments which covered with a whole genome of B.japonicum USDA 110. At 2 hrs incubation of USDA 110 with the PBS-solution, transcriptional levels of 151 DNA fragment regions were up-regulated more than three times in contrast with those in USDA 110 without the PBS-solution. A transcriptional level of a DNA region designated as BJ7062 showed nine-thousand times higher than that in untreated cell. We also confirmed up-regulation of two genes of nifB and nifN in USDA 110 cells with the PBS-solution. Furthermore, we re-confirmed up-regulation traits of these genes using RT-PCR method. Transcriptional levels of forty-three DNA regions in USDA 110 cells with the PBS-solution were down-regulated less than one-third in contrast with those of USDA 110 without the PBS-solution. We also found several Large transcriptional repression DNA regions in USDA 110 cells with the PBS-solution and their location of the transcriptional repression DNA regions were identical with those in bacteroids of soybean root nodules with USDA 110 at 5 weeks. These observations indicate PBS -solution in symbiosome obtained from soybean root nodules has several unknown plant factors to elicit or repress gene expressions of B.japonicum USDA 110.
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Research Products
(13 results)
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[Journal Article] ダイズ根粒シンビオソームのPeri-bacteroid spaceに存在する溶液を処理することで単生ダイズ根粒菌USDA110株に誘導される遺伝子応答の網羅的解析2006
Author(s)
横山 正, 市田沙智子, 大石真子, 中坪知史, 手島光平, 有馬泰紘, 大和田琢二, 南澤 究, 三井久幸, 板倉 学, 金子貴一, 田畑哲之, 佐伯和彦, 大森博文, 田島茂行, 内海俊樹, 阿部美紀子
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Journal Title
植物微生物研究会第15回研究交流会講演要旨集ISSN1341-0652 Vol. 15
Pages: 5-6
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Genome-wide Vie of Mesorhizobium and Bradyrhizobium, expression island clustered on symbiosis island and genome diversity.2005
Author(s)
Uchiumi T., Ohwada T., Kaneko T., Yokoyama T., Saeki K., Hayashi M., Tajima S., Minamizawa K.
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Journal Title
Current Plant Science and Biotechnology in Agriculture. Vol.41 Biological Nitrogen Fixation, Sustainable Agriculture and the Environment. (Edited by Y.Wang, M.Lin, Z.Tian, C.Elmerich, and W.Newton) (Springer, Netherlands.) ISBN-101-4020-3569-1
Pages: 139
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Global gene expression in Bradyrhizobium japonicum USDA 110 induced by PBS-solution isolated from peri-bacteroidal spaces in symbiosomes of soybean root nodules in terms of macroarray analysis.2005
Author(s)
Yokoyama T., Ichida S., Ohishi N., Nakatsubo S., Tejima K., Arima Y., Ohwada T., Minamisawa K., Mitsui H., Itakura M., Kaneko T., Tabata S., Saeki K., Omori H., Tajima S., Uchiumi T., Abe M.
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Journal Title
Abstract of the 15^<th> Annual Meeting for plant-Microbe Interactions Vol.15, ISSN1341-0652
Pages: 4-5
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression Island Cluster on the Symbiosis Island of the Mesorhizobium loti Genome.2004
Author(s)
Uchiumi T., Ohwada T., Itakura M., Mitui H., Nukui N., Dawadi P., Kaneko T., Tabata S., Yokoyama T., Tejima K., Saeki K., Omori H., Hayashi M., Maekawa T., Sriprang R., Murooka Y., Tajima S., Simomura K., Nomura M., Suzuki A., shimoda Y., Sioya K., Abe M., Minamisawa K
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Journal Title
J. of Bacteriol. Vol. 186
Pages: 2439-2448
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Expression Island Cluster on the Symbiosis Island of the Mesorhizobium lote Genome.2004
Author(s)
Uchiumi T., Ohwada T., Itakura M., Mitui H., Nukui N., Dawadi P., Kaneko T., Tabata S., Yokoyama T., Tejima K., Saeki K., Omori H., Hayashi M., Maekawa T., Sriprang R., Murooka Y., Tajima S., Simomura K., Nomura M., Suzuki A., shimoda Y., Sioya K., Abe M., Minamisawa K.
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Journal Title
J.of Bacteriol. Vol.186
Pages: 2439-2448
Description
「研究成果報告書概要(欧文)」より
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[Book] Current Plant Science and Biotechnology in Agriculture. Vol. 41 Biological Nitrogen Fixation, Sustainable Agriculture and the Environment. Edited by Y. Wang, M. Lin, Z. Tian, C. Elmerich, and W. Newton, ISBN-101-4020-3569-1, Springer, Netherlands.2005
Author(s)
Uchiumi T., Ohwada T., Kaneko T., Yokoyama T., Saeki K., Hayashi M., Tajima S., Minamizawa K.
Total Pages
139
Publisher
Genome-wide Vie of Mesorhizobium and Bradyrhizobium, expression island clustered on symbiosis island and genome diversity.
Description
「研究成果報告書概要(和文)」より
