2005 Fiscal Year Final Research Report Summary
Non-Calvin type autotrophic CO2 fixation cycle
| Project/Area Number |
16380057
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ISHII Masaharu The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院農学生命科学研究科, 助教授 (30193262)
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| Project Period (FY) |
2004 – 2005
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| Keywords | reductive TCA cycle / 3-HP cycle |
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| Research Abstract |
In this study, we focused on the carbon dioxide fixing enzymes within the reductive TCA cycle ; Pyruvate : ferredoxin oxidoreductase and 2-Oxoglutarate : ferredoxin oxidoreductase. As for Pyruvate : ferredoxin oxidoreductase, gene analysis was performed. Also, we constructed the gene disruption system in Hydrogenobacter thermophilus. By using the system, each one gene of two 2-Oxoglutarate : ferredoxin oxidoreductases was destroyed and the role of each gene product was clarified. In addition, ferredoxin works as an electron donor for Pyruvate : ferredoxin oxidoreductase and 2-Oxoglutarate : ferredoxin oxidoreductase reactions. Therefore, redox metabolism of ferredoxin(s) within Hydrogenobacter thermophilus was analyzed and total metabolism was grasped. Moreover, ATP : citrate reaction was clarified biochemically.
We also attempted heterologous expression of Acetyl-CoA carboxylase, which is a key carbon dioxide fixing enzyme within the 3-hydroxypropionate cycle. But, in spite of all the efforts, it has not been successful.
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