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2006 Fiscal Year Final Research Report Summary

ANALYSIS OF THE GENES ENCODING UBIQUINONE BIOSYNTHETIC ENZYMES FROM HIGHER EUKARYOTES

Research Project

Project/Area Number 16380073
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionShimane University

Principal Investigator

KAWAMUKAI Makoto  Shimane University, Life and Environmental Science, Professor, 生物資源科学部, 教授 (70186138)

Project Period (FY) 2004 – 2006
KeywordsUBIQUINONE / COQ / ANTIOXIDANT / FISSION YEAST / RESPIRATION / PRENYL TRANSFERASE
Research Abstract

The isoprenoid chain of ubiquinone (Q) is determined by transpolyprenyl diphosphate synthase in microorganisms and presumably in mammals. As mice and humans produce Q-9 and Q-10, they are expected to posses solanesyl and decaprenyl diphosphate synthases as the determining enzyme for a type of ubiquinone. Here we show that murine and human solanesyl and decaprenyl diphosphate synthases are heterotetramers composed of newly characterized hDPS1 (mSPS1) and hDLP1 (mDLP1), which were identified as orthologs of the S.pombe Dps1 and Dlp1, respectively. While hDPS1 or mSPS1 can complement the S. pombe dps1 disruptant, neither hDLP1 nor mDLP1 could complement the S. pombe dlp1 disruptant. Thus, only hDPS1 and mSPS1 are functional orthologs of SpDps1. Escherichia coli was engineered to express the murine and human SpDps1 and/or SpDlp1 homologues and their ubiquinone types were determined. While transformants expressing a single component only produced Q-8 from E. coli origin, double transformants expressing mSPS1 and mDLP1 or hDPS1 and hDLP1 produced Q-9 or Q-10, respectively, and an in vitro activity of solanesyl or decaprenyl diphosphate synthase was verified. The complex size of the human and murine long-chain transprenyl diphosphate synthases, as estimated by gel filtration chromatography, indicates that they consist of heterotetramers. Expression in E. coli of heterologous combinations, namely, mSPS1 and hDLP1 or hDPS1 and mDLP1, generated both Q-9 and Q-10, indicating both components are involved in determining the ubiquinone side chain. Thus, we identified the components of the enzymes that determine the side chain of ubiquinone in mammals.

  • Research Products

    (14 results)

All 2006 2005 2004

All Journal Article (14 results)

  • [Journal Article] Metabolic engineering of coenzyme Q by modification of isoprenoid side chain in plant.2006

    • Author(s)
      S.Takahashi, Y.Ogiyama, H.Kusano, H.Shimada, M.Kawamukai, K.Kadowaki
    • Journal Title

      FEBS Lett. 580

      Pages: 955-959

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Farnesyl and solanesyl diphosphate synthases localize to glycosomes of Trypanosoma cruzi.2006

    • Author(s)
      M.Ferella, A.Montalvetti, P.Rohloff, K.Miranda, J.Fang, S.Reina, M.Kawamukai, J.Bua, D.Nilsson, C.Pravia, A.Katzin, M.B.Cassera, L.aslund, B.Andersson, R.Docampo, E.J.Bontempi.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 39339-39348

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Metabolic engineering of coenzyme Q by modification of isoprenoid side chain in plant.2006

    • Author(s)
      Sakiko Takahashi, Yuki Ogiyama, Hiroaki Kusano, Hiroaki Shimada, Makoto Kawamukai, Koh-ichi Kadowaki.
    • Journal Title

      FEBS Lett. 580

      Pages: 955-959

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Farnesyl and solanesyl diphosphate synthases localize to glycosomes of Trypanosoma cruzi.2006

    • Author(s)
      Marcela Ferella, Andrea Montalvetti, Peter Rohloff, Kildare Miranda, Jianmin Fang, Silvia Reina, Makoto Kawamukai, Jacqueline Bua, Daniel Nilsson, Carlos Pravia, Alejandro Katzin, Maria B. Cassera, Lena aslund, Bjorn Andersson, Roberto Docampo, Esteban J. Bontempi.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 39339-39348

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans.2005

    • Author(s)
      R.Saiki, A.Nagata, T.Kainou, H.Matsuda, M.Kawamukai
    • Journal Title

      FEBS J. 56

      Pages: 5606-5622

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Batch and fed-batch production of coenzyme Q(10) in recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Gluconobacter suboxydans.2005

    • Author(s)
      Y.-C.Park, S.-J.Kim, J.-H.Cho, W.-H.Lee, K.-M.Park, M.Kawamukai, Y.-W.Ryu, J.-H.Seo
    • Journal Title

      Appl. Microbiol. Biotechnol. 67

      Pages: 192-196

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Batch and fed-batch production of coenzyme Q(10) in recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Gluconobacter suboxydans.2005

    • Author(s)
      Yong-Cheol Park, Soo-Jung Kim, Jin-Ho Choi, Won-Heong Lee, Kyung-Moon Park, Makoto Kawamukai. Yeon-Woo Ryu anc Jin-Ho Seo.
    • Journal Title

      Appl Microbiol Biotechnol. 67

      Pages: 192-196

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans2005

    • Author(s)
      Ryoichi Saiki, Ai Nagata, Tomohiro Kainou, Hideyuki Matsuda, Makoto Kawamukai.
    • Journal Title

      FEBS J 56

      Pages: 5606-5622

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The AtPPT1 gene encoding 4-hydroxybenzoate polyprenyl diphosphate transferase in ubiquinone biosynthesis is required for embryo development in Arabidopsis thaliana2004

    • Author(s)
      K.Okada, K.Ohara, K.Yazaki, K.Nozaki, N.Uchida, M.Kawamukai, H.Nojiri, H.Yamane
    • Journal Title

      Plant Mol. Biol. 55

      Pages: 567-577

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification and subcellular localization of two solanesyl diphosphate synthases from Arabidopsis thaliana.2004

    • Author(s)
      L.Jun, R.Saiki, K.Tatsumi, T.Nakagawa, M.Kawamukai
    • Journal Title

      Plant Cell Physiology 45

      Pages: 1882-1888

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular cloning and functional expression of geranylgeranyl pyrophosphate synthase from Coleus forskohlii Briq.2004

    • Author(s)
      S.Engprasert, F.Taura, M.Kawamukai, Y.Shoyama
    • Journal Title

      BMC Plant Biology 18:18

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The AtPPT1 gene encoding 4-hydroxybenzoate polyprenyl diphosphate transferase in ubiquinone biosynthesis is required for embryo development it Arabidopsis thaliana.2004

    • Author(s)
      Kazunori Okada, Kazuaki Ohara, Kazufum Yazaki, Kouhei Nozaki, Naonori Uchida, Makotc Kawamukai, Hideaki Nojiri, Hisakazi Yamane.
    • Journal Title

      Plant Mol. Biol. 55

      Pages: 567-577

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular cloning and functional expression of geranylgeranyl pyrophosphate synthase from Coleus forskohlii Brig.2004

    • Author(s)
      Surang Engprasert, Futoshi Taura, Makotc Kawamukai, Yukihiko Shoyama.
    • Journal Title

      BNC Plant Biology 18:18

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification and subcellular localization of two solanesyl diphosphate synthases from Arabidopsis thaliana.2004

    • Author(s)
      Luo Jun, Ryoichi Saiki, Kei Tatsumi, Tsuyoshi Nakagawa, Makoto Kawamukai.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 1882-1888

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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