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2006 Fiscal Year Final Research Report Summary

Brain hormones and appetite in fish

Research Project

Project/Area Number 16380139
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionKitasato University

Principal Investigator

TAKAHASHI Akiyoshi  Kitasato University, School of Fisheries Sciences, Professor, 水産学部, 教授 (10183849)

Co-Investigator(Kenkyū-buntansha) AMANO Masafumi  Kitasato University, School of Fisheries Sciences, Associate Professor, 水産学部, 准教授 (10296428)
IIGO Masayuki  Utsunomiya University, Faculty of Agriculture, Associate Professor, 農学部, 准教授 (10232109)
Project Period (FY) 2004 – 2006
KeywordsBarfin flounder / Goldfish / MCH receptor / Melanocortin receptor / Appetite regulation / Energy metabolism / Skin / Liver
Research Abstract

1. Melanin-concentrating hormone (MCH) of the barfin flounder is composed of 17 residues, which differs from salmon MCH at a position 2. The biological activity of synthetic MCH was demonstrated by the aggregation of melanosomes in flounder skin after intraperitoneal injection of the peptide. The number of MCH mRNA copies was greater in fish reared with a white background than in those reared with a black background. The MCH gene expression level in the brain was higher in fasted fish than in controls, indicating the association of MCH with food intake.
2. We cloned two MCH receptors (MCH-R) from the barfin flounder. The expression of MCH-R1 was confined to the brain, while transcripts of MCH-R2 were detected in several tissues including the brain. The expression of MCH-R2 in eyed-sideskin was higher in fish reared with a black background than with a white background.
3. In goldfish, cumulative food intake was decreased by intracerebroventricular injection of MCH, while it was increased by intracerebroventricular injection of MCH antiserum. The number of MCH immunoreactive somata in a part of the goldfish hypothalamus decreased significantly in fasted fish as compared with fed fish. These results indicate that MCH has a potential to function as an anorexigenic neuropeptide in goldfish.
4. We cloned three POMC subtypes from the barfin flounder. The expression of POMC-A was confined to the pituitary, while transcripts of POMC-B and-C were detected in several tissues including the pituitary. Posttranslational processing of POMC of the pars distalis and pars intermedia in the barfin flounder pituitary differs from each other.
5. Expressions of melanocortin 1 and 4 receptors were observed in the brain, eye-balls, testes, and skin of the barfin flounder. Melanocortin 4 receptor was also expressed in the liver, of which expression was increased by fasting. The results suggest the association of this receptor with energy homeostasis.

  • Research Products

    (46 results)

All 2007 2006 2005 2004 Other

All Journal Article (45 results) Book (1 results)

  • [Journal Article] Profiles of α-melanocyte-stimulating hormone in the Japanese flounder as revealed by a newly developed time-resolved fluoroimmunoassay and immunohistochemistry2007

    • Author(s)
      Amiya, N.
    • Journal Title

      Gen. Comp. Endocrinol. 151

      Pages: 135-141

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Feeding-induced changes of melanin-concentrating hormone (MCH)-like immunoreactivity in the goldfish brain2007

    • Author(s)
      Matsuda, K.
    • Journal Title

      Cell Tissue Res. 328

      Pages: 375-382

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Hypermelanosis on the blind side of Japanese flounder is diminished by rearing in white tank2007

    • Author(s)
      Yamanome, T.
    • Journal Title

      Fish. Sci. 73

      Pages: 466-468

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Further evidence for ancient role of ACTH peptides at melanocortin (MC) receptors; pharmacology of dogfish and lamprey peptides at dogfish MC receptors2007

    • Author(s)
      Haitina, T.
    • Journal Title

      Peptides 28

      Pages: 798-805

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The melanin-concentrating hormone receptor 2 (MCH-R2) mediates the effect of MCH to control body color for background adaptation in the barfin flounder2007

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen. Comp. Endocrinol. 151

      Pages: 210-219

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] cDNA cloning of proopiomelanocortin (POMC) and mass spectrometric identification of POMC-derived peptides from snake and alligator pituitaries2007

    • Author(s)
      Kobayashi, Y.
    • Journal Title

      Gen. Comp. Endocrinol. 152

      Pages: 73-81

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of background color on GnRH and MCH levels in the barfin flounder brain2007

    • Author(s)
      Amiya, N.
    • Journal Title

      Gen. Comp. Endocrinol. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Profiles of a-melanocyte-stimulating hormone in the Japanese flounder as revealed by a newly developed time-resolved fluoroimmunoassay and immunohistochemistry2007

    • Author(s)
      Amiya, N.
    • Journal Title

      Gen.Comp.Endocrinol. 151

      Pages: 135-141

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hypermelanosis on the blind side of Japanese flounder is diminished by rearing in white tank2007

    • Author(s)
      Yamanome, T.
    • Journal Title

      Fish.Sci. 73

      Pages: 466-468

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Further evidence for ancient role of ACTH peptides at melanocortin (MC) receptors ; pharmacology of dogfish and lamprey peptides at dogfish MC receptors2007

    • Author(s)
      Haitina, T.
    • Journal Title

      Peptides 28

      Pages: 798-805

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The melanin-concentrating hormone receptor 2 (MCH-R2) mediates the effect of MCH to control body color for background adaptation in the barfin flounder2007

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen.Comp.Endocrinol. 151

      Pages: 210-219

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] cDNA cloning of proopiomelanocortin (POMC) and mass spectrometric identification of POMC-derived peptides from snake and alligator pituitaries2007

    • Author(s)
      Kobayashi, Y.
    • Journal Title

      Gen.Comp.Endocrinol. 152

      Pages: 73-81

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of background color on GnRH and MCH levels in the barfin flounder brain2007

    • Author(s)
      Amiya, N.
    • Journal Title

      Gen.Comp.Endocrinol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] プロオピオメラノコルチンの分子進化2006

    • Author(s)
      高橋明義
    • Journal Title

      生体の科学 57

      Pages: 424-425

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of three proopiomelanocortin subtype genes and mass spectrometric identification of POMC-derived peptides in pars distalis and pars intemedia of barfin flounder pituitary2006

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen. Comp. Endocrinol. 145

      Pages: 280-286

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A systematic immunohistochemical survey on the distribution patterns of GH, prolactin, somatolactin, β-TSH, β-FSH, β-LH, ACTH, and α-MSH in the adenohypophysis of Oreochromis niloticus, the Nile tilapia2006

    • Author(s)
      Kasper, R.S.
    • Journal Title

      Cell Tissue Res. 325

      Pages: 303-313

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular cloning and characterization of preproopiomelanocortin (prePOMC) cDNA from the ostrich (Struthio camelus)2006

    • Author(s)
      Naude, R.
    • Journal Title

      Gen. Comp. Endocrinol. 146

      Pages: 310-317

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Occurrence of two functionally distinct proopiomelanocortin genes in all modern lampreys2006

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen. Comp. Endocrinol. 148

      Pages: 72-78

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Posttranslational processing of proopiomelanocortin family molecules in sea lamprey based on mass spectrometric and chemical analyses2006

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen. Comp. Endocrinol. 148

      Pages: 79-84

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Evolution of melanocortin systems in fish2006

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen. Comp. Endocrinol. 148

      Pages: 85-94

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Central administration of melanin-concentrating hormone (MCH) suppresses food intake, but not locomotor activity, in the goldfish, Carassius auratus2006

    • Author(s)
      Matsuda, K.
    • Journal Title

      Neurosci. Lett. 399

      Pages: 259-263

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular evolution of proopiomelanocortin2006

    • Author(s)
      Takahashi, A.
    • Journal Title

      Seitaino Kagaku 57(in Japanese)

      Pages: 424-424

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of three proopiomelanocortin subtype genes and mass spectrometric identification of POMC-derived peptides in pars distalis and pars intemedia of barfin flounder pituitary.2006

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen.Comp.Endocrinol. 145

      Pages: 280-286

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A systematic immunohistochemical survey on the distribution patterns of GH, prolactin, somatolactin, β-TSH, β-FSH, β-LH, ACTH, and α-MSH in the adenohypophysis of Oreochromis niloticus, the Nile tilapia2006

    • Author(s)
      Kasper, R.
    • Journal Title

      Cell Tissue Res. 325

      Pages: 303-313

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular cloning and characterization of preproopiomelanocortin (prePOMC) cDNA from the ostrich (Struthio camelus)2006

    • Author(s)
      Naude, R.
    • Journal Title

      Gen.Comp.Endocrinol. 146

      Pages: 310-317

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Occurrence of two functionally distinct proopiomelanocortin genes in all modern lampreys2006

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen.Comp.Endocrinol. 148

      Pages: 72-78

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Posttranslational processing of proopiomelanocortin family molecules in sea lamprey based on mass spectrometric and chemical analyses2006

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen.Comp.Endocrinol. 148

      Pages: 79-84

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Evolution of melanocortin systems in fish2006

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen.Comp.Endocrinol. 148

      Pages: 85-94

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] White background reduces the occurrence of staining, activates melanin-concentrating hormone and promotes somatic growth in barfin flounder2005

    • Author(s)
      Yamanome, T.
    • Journal Title

      Aquaculture 244

      Pages: 323-329

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunocytochemical localization and ontogenic development of α-melanocyte-stimulating hormone (α-MSH) in the brain of a pleuronectiform fish, barfin flounder2005

    • Author(s)
      Amano, M.
    • Journal Title

      Cell Tissue Res. 320

      Pages: 127-134

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Nucleotide sequence and expression of three subtypes of proopiomelanocortin mRNA in barfin flounder2005

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen. Comp. Endocrinol. 141

      Pages: 291-303

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of tank color on melanin-concentrating hormone levels in the brain, pituitary gland and plasma of the barfin flounder as revealed by a newly developed time-resolved fluoroimmunoassay2005

    • Author(s)
      Amiya, N.
    • Journal Title

      Gen. Comp. Endocrinol. 143

      Pages: 251-256

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Structures for the proopiomelanocortin family genes proopiocortin and proopiomelanotropin in the sea lamprey Petromyzon marinus2005

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen. Comp. Endocrinol. 144

      Pages: 174-181

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Promoter activity of sea lamprey proopiocortin and proopiomelanofropin genes in AtT-20/D16v cells2005

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen. Comp. Endocrinol. 144

      Pages: 182-187

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunocytochemical localization and ontogenic development of a-melanocyte-stimulating hormone (α-MSH) in the brain of a pleuronectiform fish, barfin flounder2005

    • Author(s)
      Amano, M.
    • Journal Title

      Cell Tissue Res. 320

      Pages: 127-134

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Nucleotide sequence and expression of three subtypes of proopiomelanocortin mRNA in barfin flounder2005

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen.Comp.Endocrinol. 141

      Pages: 291-303

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of tank color on melanin-concentrating hormone levels in the brain, pituitary gland and plasma of the barfin flounder as revealed by a newly developed time-resolved fluoroimmunoassay2005

    • Author(s)
      Amiya, N.
    • Journal Title

      Gen.Comp.Endocrinol. 143

      Pages: 251-256

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Structures for the proopiomelanocortin family genes proopiocortin and proopiomelanotropin in the sea lamprey Petromyzon marinus2005

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen.Comp.Endocrinol. 144

      Pages: 174-181

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Promoter activity of sea lamprey proopiocortin and proopiomelanotropin genes in AtT-20/D16v cells2005

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen.Comp.Endocrinol. 144

      Pages: 182-187

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular cloning of proopiomelanocortin cDNA in the ratfish, a holocephalan2004

    • Author(s)
      Takahashi, A
    • Journal Title

      Gen. Comp. Endocrinol. 135

      Pages: 159-165

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Possible involvement of melanin-concentrating hormone in food intake in a teleost fish, barfin flounder2004

    • Author(s)
      Takahashi, A
    • Journal Title

      Peptides 25

      Pages: 1613-1622

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular cloning of proopiomelanocortin cDNA in the ratfish, a holocephalan2004

    • Author(s)
      Takahashi, A.
    • Journal Title

      Gen.Comp.Endocrinol. 135

      Pages: 159-165

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Possible involvement of melanin-concentrating hormone in food intake in a teleost fish, barfin flounder2004

    • Author(s)
      Takahashi, A.
    • Journal Title

      Peptides 25

      Pages: 1613-1622

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Central administration of melanin-concentrating hormone (MCH) suppresses food intake, but not locomotor activity, in the goldfish, Carassius auratus

    • Author(s)
      Matsuda, K.
    • Journal Title

      Neurosci.Lett. 399

      Pages: 259-263

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Diverse structures and functions of melanocortin, endorphin and melanin-concentrating hormone in fish

    • Author(s)
      Takahashi, A.
    • Journal Title

      In "Fish Endocrinology" (G.Zaccone, M.Reinecke, B.G.Kapoor, Eds.), Science Publishers. Enfield

      Pages: 871

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Fish Endocrinology2006

    • Author(s)
      Takahashi, A.
    • Total Pages
      871
    • Publisher
      Science Publishers
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2008-05-27  

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