2006 Fiscal Year Final Research Report Summary
Basic study on the production of somatic nuclear transferred chicken.
| Project/Area Number |
16380184
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | University of Tsukuba |
|---|
Principal Investigator |
TAJIMA Atsushi University of Tsukuba, Graduate School of Life and Environmental Sciences, Associate Professor, 大学院生命環境科学研究科, 助教授 (40207030)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAITO Mitsuru Development and Differentiation Group, National Institute of Agro-Bioresources, Team Leader, 発生分化研究グループ発生制御研究チーム, チーム長(研究職) (70355733)
|
|---|
| Project Period (FY) |
2004 – 2006
|
| Keywords | Primordial Germ Cells / Nuclear transfer / chicken |
|---|
| Research Abstract |
1. Production of nuclear transferred GGC.:
Attempts were made to produce nuclear transferred gonadal germ cells (GGCs) using karyoplast produced from fibroblast. Karyoplasts were produced by using centrifuging fibroblast at 10,000 g 1 hour in the presence of 0.1% cytochalasin B. Average diameter of the karyoplasts were 10% smaller tcompared with intact fibroblast. Six nuclear transferred GGCs were produced using karyoplasts by electrofusion.
2. Production of intra-Genus nuclear transferred GGCs.
Attempts were made to produce intra-genus nuclear transferred GGC using chicken and quail. After electrofusion, 0.5-1.2 % of the nuclear GGCs was produced.
3. Effects of soft X exposure on the migrating ability of primordial germ cells.
Effects of soft X exposure on the migrating ability of primordial germ cells were examined. Results showed that the PGCs retain their migration ability toward gonadal ridge after transfer even after soft exposure.
|
