2006 Fiscal Year Final Research Report Summary
Functional analysis of phosphatidyliositol-bindin proteins in fission yeast
| Project/Area Number |
16380231
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kagawa University |
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Principal Investigator |
TAKEGAWA Kaoru Kagawa University, Department of Agriculture, Professor, 農学部, 助教授 (50197282)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Naotaka Kagawa University, Department of Agriculture, Assistant Professor, 農学部, 助教授 (60324109)
SUGIURA Reiko Kinki University, School of Pharmaceutical Sciences, Professor, 薬学部, 教授 (90294206)
NAKAMURA Taro Osaka City University, Department of Biology, Lecturer, 理学研究科, 講師 (30291082)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Fission yeast / Phosphatidylinositol / vesicle trafficking / sporulation / endocytosis / PX domain / FYVE domain |
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| Research Abstract |
Recent progress in the Schizosaccharomyces pombe genome project has revealed that most of genes required for the synthesis of phosphoinositides are conserved between S.pombe and Saccharomyces cerevisiae. We have cloned the gene, vps34/pik3, encoding a phosphatidylinositol (PtdIns) 3-kinase from S.pombe. In this project, we have identified and characterized PX and FYVE domain-containing proteins in S. pombe.
1.Among various PX domain-containing proteins, Vps5p and Vps17p, homologues of sorting nexins, were found to be required for efficient sporulation, vacuolar protein sorting and vacuolar biogenesis in S.pombe cells.
2.The FYVE-domain containing Ste12 protein catalyzes a PtdIns 3-P 5-kinase, which is required for efficient mating. Suppressor mutants for sterility of ste12 cells were identified. Of these, sst4/vps27 has been identified. The fission yeast Sst4p contain FYVE domain, and Sst4p is shown to be required for vacuolar protein and multivesicular sortings in S.pombe.
3.Valproic acid (VPA) is widely used to treat epilepsy and manic-depressive illness. A genetic screen for fission yeast mutants that show hypersensitivity to VPA was isolated. One of the genes that we identified was vps45+, which encodes a member of Sec1/Munc18 family that is implicated PtdIns-dependent membrane trafficking.
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[Journal Article] Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe.2007
Author(s)
Iwaki, T., Onishi, M., Ikeuchi, M., Kita, A., Sugiura, R.Giga-Hama, Y., Fukui, Y., Takegawa, K
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Journal Title
Microbiology 175,(in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Valpronic acid affects trafficking and cell wall integrity in fission yeast.2007
Author(s)
Miyatake, M., Kuno, T., Kita, A., Katsura, K.Takegawa, K., Nabata, T., Sugiura, R.
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Journal Title
Genetics 175
Pages: 1695-1705
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic Evidence for Phospholipid-Mediated Regulation of the Rab GDP-Dissociation Inhibitor in Fission Yeast.2006
Author(s)
Ma, Y., Kuno, T., Kita, A., Nabata, T., Uno, S., Sugiura, R.
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Journal Title
Genetics 174
Pages: 1259-1271
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] An alpha-amylase homologue, aah3+, encodes a GPI-anchored membrane protein required for cell wall integrity in Schizosaccharomyces pombe.2006
Author(s)
Morita, T., Tanaka, N., Hosomi, A., Giga-Hama, Y, Takegawa, K.
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Journal Title
Biosci.Biotechnol.Biochem. 70
Pages: 1454-1463
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Vacuolar protein sorting receptor in Schizosaccharomyces pombe.2006
Author(s)
Iwaki, T., Hosomi, A., Tokudomi, S., Kusunoki, Y., Fujita, Y., Giga-Hama, Y, Tanaka, N., Takegawa, K.
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Journal Title
Microbiology 152
Pages: 1523-1532
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic and functional interaction between Ryh1 and Ypt3 : two Rab GTPases that function in S. pombe secretory pathway.2006
Author(s)
He, Y., Sugiura, R., Ma, Y., Kita, A., Deng, L., Takegawa, K., Matsuoka, K., Shuntoh, H., Kuno, T.
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Journal Title
Genes Cells 11
Pages: 207-221
Description
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[Journal Article] Phosphatidylinositol-4-phosphate-5-kinase regulates fission yeast cell integrity through a phospholipase C-mediated protein kinase C-independent pathway.2005
Author(s)
Deng, L., Sugiura, R., Ohta, K., Tada, K., Suzuki, M., Hirata, M., Nakamura, S., Shuntoh, H., Kuno, T.
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Journal Title
J.Biol.Chem. 280
Pages: 27561-27568
Description
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[Journal Article] Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe.2004
Author(s)
Koga, T., Onishi, M., Nakamura, Y., Hirata, A., Nakamura, T., Shimoda, C., Iwaki, T., Takegawa, K., Fukui, Y.
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Journal Title
Genes Cells 9
Pages: 561-574
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of end4+, a gene required for endocytosis in Schizosaccharomyces pombe.2004
Author(s)
Iwaki, T., Tanaka, N., Takagi, H., Giga-Hama, Y., Takegawa, K.
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Journal Title
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「研究成果報告書概要(欧文)」より
