2005 Fiscal Year Final Research Report Summary
Elucidation of the relation between multi-ligand receptor, FEEL and diseases
| Project/Area Number |
16390027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | RIKEN |
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Principal Investigator |
ADACHI Hideki Riken, Cellular Biochemistry Laboratory, Researcher, 辻本細胞生化学研究室, 先任研究員 (60291051)
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| Project Period (FY) |
2004 – 2005
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| Keywords | FEEL / arteriosclerosis / diabetes |
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| Research Abstract |
Multiligand receptors (scavenger receptors) are defined as its ability to recognize several structurally and functionally dissimilar molecule such as modified lipoproteins, advanced glycation end-products (AGE) and apoptotic cells. It is suggested that they were implicated in various diseases such as arteriosclerosis and diabetes. Employing expression cloning, we have cloned a multiligand receptor termed FEEL-1 (fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavenger receptor-1) expressed in human endothelial cells and subsequently cloned FEEL-2 by data base search. The receptors had binding activities to modified lipoproteins, cells of both Gram-positive, Gram-negative bacteria and AGE. Soluble form of human FEEL-1 was expressed using adenoviral vector, and the binding activities of soluble form of FEEL-1 to acetyl-LDL or AGE were determined. Expression of miRNA (micro RNA) against FEEL employing fiber mutant adenoviral vector in murine liver sinusoidal endothelial cells suggested that both FEEL-1 and FEEL-2 contribute to the hepatic reticuloendothelial system in clearance of waste materials from blood flow. Knockout mice of FEEL-1 or FEEL-2 were generated by gene targeting. Determination of the physiological function of FEEL will provide a new insight into the pathological function of this multiligand receptor.
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