2005 Fiscal Year Final Research Report Summary
Discovery of targets for treatment with renal cell carcinoma by gene and protein expression profile analysis
| Project/Area Number |
16390040
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Kobe University |
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Principal Investigator |
OKUMURA Katsuhiko Kobe University, Kobe University Hospital, School of Medicine, Professor, 医学部附属病院, 教授 (60025707)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAEDA Toshiyuki Kobe University, Kobe University Hospital, School of Medicine, Associate Professor, 医学部附属病院, 助教授 (00304098)
GOTOH Akinobu Hyogo University, School of Medicine, Professor, 医学部, 教授 (70283885)
SHIRAKAWA Toshiro Kobe University, School of Medicine, Associate Professor, 医学部, 助教授 (70335446)
OKAMURA Noboru Kobe University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (60379401)
NAKAMURA Tsutomu Kobe University, Kobe University Hospital, School of Medicine, Pharmacist, 医学部附属病院, 薬剤師 (80379411)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Proteome analysis / Transcriptome analysis / Renal cell carcinoma / Sorcin / VEGF / MALDI-TOF / Ms |
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| Research Abstract |
Renal cell carcinoma (RCC) is one of the most chemotherapy or radiotherapy-resistant malignant tumors, and therefore, the standard therapy is surgical treatment. Also, there is no efficient biomarker for diagnosis. To elucidate resistant mechanisms and discovery novel targets for treatment and biomarker for diagnosis, transcriptome and proteome analysis were carried out using RCC and adjacent normal tissues.
Since lower expression of sorcin in RCC was found, we investigated its role in cancer proliferation by RNA interference. Up-regulation of vascular endothelial growth factor (VEGF), a potent angiogenesis factor contributing to cancer proliferation, was found by knock-down of sorcin using siRNA in renal cell carcinoma cells, Caki-1. This suggested that lower expression of sorcin caused up-regulation of VEGF, thereby lead cancer proliferation.
To comprehensive analysis of protein expressions in RCC and adjacent normal tissues, samples were labeled with 2-nitrobeazenesulfonyl chloride with six ^<12>C or ^<13>C atoms. Then, they were applied MALDI-TOF/Ms and pair peak of 6 Da difference were analyzed and identified. Unknown proteins were identified in addition to known proteins, which have been reported to be up-regulated in RCC.
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