2006 Fiscal Year Final Research Report Summary
ANALYSIS OF PROTEINS SPECIFICALLY EXPRESSED BY LYMPHATIC VESSEL SAND MECHANISMS OF LYMPHAYGIOGENESIS AND LYMPH-DEGENERATION
| Project/Area Number |
16390047
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | University of Toyama (2005-2006)
Toyama Medical and Pharmaceutical University (2004) |
|---|
Principal Investigator |
OHTANI Osamu University of Toyama, GRADUATE SCHOOL FOR MEDICINE AND PHARMACEUTICAL SCIENCES FOR RESEARCH, professor (90127548)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHTANI Yuko UNIVERSITY OF TOYAMA, GRADUATE SCHOOL FOR MEDICINE AND PHARMACEUTICAL SCIENCES FOR RESEARCH, assistant professor (10313595)
MATSUO Mitsuhiro UNIVERSITY OF TOYAMA, GRADUATE SCHOOL FOR MEDICINE AND PHARMACEUTICAL SCIENCES FOR RESEARCH, ASSISTANT PROFESSOR (70361954)
|
|---|
| Project Period (FY) |
2004 – 2006
|
| Keywords | LYMPHATIC VESSELS / LYMPHATIC ENDOTHELIAL CELLS / LYMPHANGIOGENESIS / LYMPH-REGENERATTON / LOW OXYGEN / CELL CULTURE / LYVE-1 / VEGFR-3 |
|---|
| Research Abstract |
We have firstly employed immunohistochemistry using antibodies to markers specific to lymphatic vessels, including LYVE-1, VEGER-3 and Prox-1 to verify the distribution of the lymphatic vessels in a variety of organs that include the thyroid gland, lung, pleura, diaphragm, liver, intestine, Beyer's patches, appendix, tonsil and lymph node and the development of lymphatic vessels in the diaphragm. In the diaphragm lymphatic vessels develop primarily by sprouting, but prior to the formation of tubular vessels, numerous lymphatic endothelial cells(LECs) independently migrate in line to the place where lymphatic vessels will form, and later they join together to form lymphatic vessels. Secondly, we have performed some experiments using LECs harvested from the thoracic duct and cisterna chyli of the green rats. The LECs were proved to express LYVE-1 and VEGFR-3 by Western blotting and immunohistochemistry. The LECs proliferate faster in a low oxygen environment than in normal one. When cultured on collagen fiber network with natural organization, LECs rapidly form lymphatic plexus. LECs implanted in the peritoneal cavity enter the lymphatic vessels in the diaphragm. Some of the LECs implanted are incorporated into the preexisting recipient's lymphatic vessels, and others form either new lymphatic vessels or nodules. This suggests the possibility that implanted LECs participate in the formation of lymphatic vessels. When CD34^+ cells isolated from the blood of the green rats are cultured on Cell Culture Inserts[○!R] in a low oxygen condition, they form tubular structures similar to lymphatic vessels, suggesting existence of progenitor cells of LECs in the blood.
|
Research Products
(16 results)
-
-
-
-
-
-
[Journal Article] ESTABLISHIMENT AND STABILIZATION OF CONDITIONALLY IMMORTALIZED ENDOTHELIAL CELL LINES FROM THE THORACIC DUCT AND INFERIOR VENA CAVA OF tsA58/EGFP DOUBLE-TRANSGENIC RATS.2006
Author(s)
MITSUHIRO MATSUO, KEITCHI KOIZUMI, SANAE YAMADA, MASATOSHI TOMI, RI-ICHI TAKAHASHI, MASATSUGU UEDA, TETSUYATERASAKI, MASUO OBINATA, KEN-ICHI HOSOYA, OSAMU OHTANI, IKUO SAIKI
-
Journal Title
CELL TISSUE RES 326
Pages: 749-758
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
[Presentation] リンパ管新生の形態学2007
Author(s)
大谷 修, 大谷 裕子
Organizer
第48回日本脈管学会総会
Place of Presentation
松本
Year and Date
2007-09-25
Description
「研究成果報告書概要(和文)」より
-
-
[Presentation] 横隔膜のリンパ管2007
Author(s)
大谷 修, 大谷 裕子
Organizer
第31回日本リンパ学会総会
Place of Presentation
仙台
Year and Date
2007-06-08
Description
「研究成果報告書概要(和文)」より
-
-
[Presentation] リンパ管の新生・再生2006
Author(s)
大谷 裕子, 大谷 修
Organizer
第30回日本リンパ学会総会
Place of Presentation
東京
Year and Date
2006-06-01
Description
「研究成果報告書概要(和文)」より
