2005 Fiscal Year Final Research Report Summary
Molecular basis of Zic-mediated developmental control
| Project/Area Number |
16390086
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | RIKEN |
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Principal Investigator |
ARUGA Jun RIKEN BSI, Laboratory for Comparative.Neurogenesis, Laboratory Head, 比較神経発生研究チーム, チームリーダー (10232076)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIGURO Akira RIKEN BSI, Laboratory for Comparative.Neurogenesis, Research Scientist, 比較神経発生研究チーム, 研究員 (70373264)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Zic / transcription factor / cell differentiation / neural development / gene targeting / transcriptional complex / protein modification / congenital anomaly |
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| Research Abstract |
Zic genes are known for causal genes of holoprosencephaly, Dandy-Walker malformation, and heterotasxy.. In this study, following points of the Zic gene family have been dealt with. (1) Identification of novel Zic-binding proteins, (2) The biological significance of the protein-to-protein interaction between Zic and Zic-binding proteins, (3) Post-translational modification of Zic proteins, (4) Bio-signals that have contact points with Zic proteins.
Following points were revealed and publicized in the scientific community.
(1) Identification of novel two Zic2-containing high-molecular-weight molecular complexes (Ishiguro et al.,2007). Identification of I-mfa as a Zic-repressor by yeast two-hybrid screening. (2,3) Identification of a Zic2-kinase in the HMW molecular complexes. The phosphorylation of Zic2 protein was shown to be critical in the transcriptional regulatory processes. Other modifications sites in Zic proteins have been identified and characterized by mass spectrometry analysis. (4) The Zic related bio-signals was investigated by analyzing the Zic1/3 and Zic2/3 compound mutant mice (Inoue et al., 2007a; 2007b). In particular, we revealed that Zic1 and Zic3 have critical roles in the expansion of forebrain neuronal precursors. Wnt signals was suggested to be involved in the Zic mediated developmental control. In addition, functional similarity and difference among vertebrates Zic genes have been investigated in mouse and Xenopus (Ishiguro et al.,2004; Fujimi et al.,2006).
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[Journal Article] Elucidation of penetrance variability of a ZIC3 mutation in a family with complex heart defects and functional analysis of ZIC3 mutations in the first zinc finger domain.2007
Author(s)
Chhin B, Hatayama M, Bozon D, Ogawa M, Schon P, Tohmonda T, Sassolas F, Aruga J, Valard AG, Chen SC, Bouvagnet P
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Journal Title
Hum Mutat 28
Pages: 563-570
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A wide-range phylogenetic analysis of Zic proteins : implications for correlations between protein structure conservation and body plan complexity.2006
Author(s)
Aruga J, Kamiya A, Takahashi H, Fujimi TJ, Shimizu Y, Ohkawa K, Yazawa S, Umesono Y, Noguchi H, Shimizu T, Saitou N, Mikoshiba K, Sakaki Y, Agata K, Toyoda A
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Journal Title
Genomics 87
Pages: 783-792
Description
「研究成果報告書概要(欧文)」より
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[Patent(Industrial Property Rights)] 特許2005
Inventor(s)
有賀 純
Industrial Property Rights Holder
独立行政法人理化学研究所
Industrial Property Number
特許公開2006-325524
Filing Date
2005-05-27
Acquisition Date
2006-12-07
Description
「研究成果報告書概要(和文)」より
