2006 Fiscal Year Final Research Report Summary
Study of analysis for vascular damage due to embolism and inflammation
| Project/Area Number |
16390161
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
WAKAMIYA Nobutaka Asahikawa Medical College, Faculty of Medicine, Professor, 医学部, 教授 (20210867)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUZAWA Jun Faculty of Asahikawa Medical College, Faculty of Medicine, Research Assistant, 医学部, 助手 (40333695)
SUZUKI Yasuhiko Hokkaido University, Research Center for Zoonosis Control, Professor, 人獣共通感染症リサーチセンター, 教授 (90206540)
YOSHIDA Itsuro Asahikawa Medical College, Faculty of Medicine, Associate Professor, 医学部, 助教授 (20041816)
OGASAWARA Masahiro Asahikawa Medical College, Faculty of Medicine, Research Assistant, 医学部, 助手 (40185492)
OHTANI Katsuki Asahikawa Medical College, Faculty of Medicine, Research Assistant, 医学部, 助手 (90396367)
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| Project Period (FY) |
2004 – 2006
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| Keywords | collectin / analysis / blood |
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| Research Abstract |
CL-P1 is a new collectin which was found by Ohtani at 2001. It was expressed in vascular endothelial cells in murine tissues and also found in vascular endothelial cell lines. Here we established the antibody against above CL-P1 to investigate their biological functions. We have immunized mice with recombinant CL-P1 protein and made hybridoma for monoclonal antibodies. Several monoclonal antibodies were established and were divided to two or three groups. We tried to make the analysis method to detect CL-P1 using above monoclonal antibodies. We made several prototype assay systems but we could not get a good result because of less CL-P1 volume. Now we began to make a new analysis system using polyclonal antibodies.
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[Journal Article] Specific recognition of the collagen triple helix by chaperone HSP47. II. Tne HSP47-binding structural motif in collagens and related proteins2006
Author(s)
Koide T, Nishikawa Y, Asada S, Yamazaki CM, Takahara Y, Homma DL, Otaka A, Ohtani K, Wakamiya N, Nagata K, Kitagawa K
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Journal Title
J Biol Chem. 281
Pages: 11177-11185
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Identification and characterization of a novel human collectin CL-K12006
Author(s)
Keshi H, Sakamoto T, Kawai T, Ohtani K, Katoh T, Jang S-J, Motomura W, Yoshizaki T, Fukuda M, Koyama S, Fukuzawa J, Fukuoh A, Yoshida I, Suzuki Y, Wakamiya N
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Journal Title
Microbiol. Immunol. 50(12)
Pages: 1001-1013
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Identification and characterization of a novel human collectin CL-K1.2006
Author(s)
Keshi H, Sakamoto T, Kawai T, Ohtani K, Katoh T, Jong S-J, Motomura W, Yoshizaki T, Fukuda M, Koyama S, Fukuzawa J, Fukuoh A, Yoshida I, Suzuki Y, Wakamiya N.
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Journal Title
Microbiol.Immunol. 50(12)
Pages: 1001-1013
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47-binding structural motif in collagens and related proteins.2006
Author(s)
Koide T, Nishikawa Y, Asada S, Yamazaki CM, Takahara Y, Homma DL, Otaka A, Ohtani K, Wakamiya N, Nagata K, Kitagawa K.
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Journal Title
J.Biol.Chem. 281(16)
Pages: 11177-85
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Kato, S. : Preparation of monoclonal antibodies cross- reactive with Orthopoxviruses and their application for direct immunofluorescence test.2005
Author(s)
Kitamoto, N., Kobayashi, T., Kato, Y., Wakamiya, N, Ikuta, K., Tanaka, T., Ueda, S., Miyamoto, H.
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Journal Title
Microbiol.Immunol. 49(3)
Pages: 219-225
Description
「研究成果報告書概要(欧文)」より
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[Book] シンプル微生物学2006
Author(s)
東匡伸, 小熊恵二編集(分担執筆)
Total Pages
409
Publisher
南山堂
Description
「研究成果報告書概要(和文)」より
