Developmental research of biochemical test based on the molecular biological analysis of sick house syndrome

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 16390167
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Asahikawa Medical College
• Principal Investigator: YOSHIDA Takahiko Asahikawa Medical College, Professor, 医学部, 教授 (90200998)
• Co-Investigator(Kenkyū-buntansha): ITOH Toshihiro Asahikawa Medical College, Assistant Professor, 医学部, 講師 (20271760) ; NAKAGI Yoshihiko Asahikawa Medical College, Assistant, 医学部, 助手 (90322908) ; SAKABE Koh Kitazato University, Professor, 薬学部, 教授 (70162302) ; KOJIMA Hiroyuki Hokkaido Institute of Public Health, researcher, 研究職員 (10414286)
• Project Period (FY): 2004 – 2005
• Keywords: sick house syndrome / multiple chemical sensitivity / peripheral blood mono nucleocyte / DNA micro alley analysis / marker gene / mRNA / biomarker / bio chemical analysis

Research Abstract

There is no significant biochemical test for multiple chemical sensitivity (MCS). Practicable medical facilities for diagnosis of MCS are limited since a clean room is necessary to confirm the causative agent. The aim of this research is the establishment of the diagnostic test for MCS using biochemical test of peripheral blood samples. Sixteen patients diagnosed as MCS, 9 healthy subjects and 4 subjects for control were enrolled in this research. Pooled total RNA obtained from peripheral blood mononuclear cells (PBMCs) of control subjects and similarly prepared total RNA from each MCS patient or healthy subject were labeled with different color fluorescence respectively, and thoroughly explored the marker gene by DNA micro alley analysis. The gene with over than 2 times or less than half expression in the MCS patients in contrast with no change in the healthy subjects was judged as a varied gene. Although there was no gene which varied in all MCS patients, 28 genes (increase:2,decreas e:26) were detected as varied genes among over half MCS patients. Ten distinguished genes were identified in the analysis between the control and MCS patients whose gene expression level was high. Five genes of them were also identified as decreasingly varied genes in PBMCs or spleen cells from the mouse which were exposed to formaldehyde (HCHO). Those genes were possibly to be diagnostic marker genes, since they were thought to be varied by stress, although the correlation between those genes and the occurrence of MCS remained unclear. We considered the possibility to detect the proteins as gene products in serum. But those products were enzymes or receptors expressed in tissue or cell and unable to detect in blood, or cytokines which released into blood at too low level for measuring. It is failed to be established the general biochemical test for MCS diagnosis in this study. Further researches using the protein chip methods are required to focus the biomarker produced and poured into blood by liver, kidney or nerves systems.