Indentification and functional analysis of novel gastrointestinal peptides involved in the regulation of feeding of energy homeostasis

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 16390212
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: University of Miyazaki
• Principal Investigator: NAKAZATO Masamitsu UNIVERSITY OF MIYAZAKI, FACULTY OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 教授 (10180267)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Hideki UNIVERSITY OF MIYAZAKI, FACULTY OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 助手 (10305097) ; MURAKAMI Noboru UNIVERSITY OF MIYAZAKI, FACULTY OF AGRICULTURE, PROFESSOR, 農学部, 教授 (80150192) ; KANGAWA Kenji NATIONAL CARDIOVASCULAR CENTER RESEARCH INSTITUTE, DIRECTOR GENERAL, 所長 (00112417) ; DATE Yukari UNIVERSITY OF MIYAZAKI, FACULTY OF MEDICINE, PROFESSOR, フロンティア科学実験センター, 教授 (70381100) ; TOSHINAI Koji UNIVERSITY OF MIYAZAKI, FACULTY OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 助手 (80381101)
• Project Period (FY): 2004 – 2006
• Keywords: novel peptides / feeding regulation / energy homeostasis / gastrointestinal endocrine cells / mass spectrometry / ghrelin / NERP / グレリン

Research Abstract

Exhaustive analyses of cultured cells using Peptidome and peptidmics will identify a variety of peptide fragments derived from a precursor protein and will enable the assumption of physiologically active peptides based on genetic information on the precursor protein and the amino acid sequences of the newly identified peptide fragments. Actually, when cultured digestive tract cells were analyzed using these technologies, 250 peptide fragments were orderly identified. By using these fragments, prospectively significant peptides were synthesized to assess their bioactivities of the targeted cells. After binding to its receptor, a physiologically active peptide changes the levels of intracellular calcium, cyclic AMP, and cyclic GMP as well as the amount of arachidonic acid metabolite and intracellular pH values to convey information. Following the administration of candidate peptides to the above-mentioned digestive tract cells, we performed in vitro screening to identify physiologically active peptides involved in biological regulation using fluorescence cellular imaging analysis, which employed fluorescent staining and a CCD camera for analyzing overtime changes in membrane electric field and the intracellular signal transmission system; and thus we discovered novel peptides, NERP-1 and NERP-2, in humans and rats. Through genetic engineering, ethopharmacology, and the development of exhaustive analytical technique, we elucidated their physiological implication that both NERPs regulate energy metabolism, water/electrolyte homeostasis, and the autonomic nervous system. Moreover, we have established a high-through put cell-based assay system that enables assessment of the dynamics of the intracellular signal transmission system, which contributed to the identification of 4 kinds of novel neuropeptides. These peptides are undergoing functional analysis. We have started translational medicine aiming to apply the appetite and GH secretion stimulatory effects of gastric ghrelin to treatment for patients with chronic obstructive lung disorder and for those with lower respiratory tract infection. Ghrelin has been demonstrated to have energy anabolic property as well as to enhance cellular regeneration and prevent inflammation.

Research Products

• [Journal Article] Long-term treatment with theophylline reduces neutrophils, interleukin-8 and tumor necrosis factor-a in the sputum of patients with chronic obstructive pulmonary disease. (2007)
  • Author(s): Iiboshi H, Ashitani J, Katoh S, Sano A, Matsumoto N, Mukae H, Nakazato M
  • Journal Title: Pulm Pharmacol Ther 20 Pages: 46-51
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Elevated levels of antimicrobial peptides in bronchoalveolar lavage fluid in patients with chronic eosinophilic pneumonia. (2007)
  • Author(s): Ashitani J, Matsumoto N, Nakazato M
  • Journal Title: Respiration 74 Pages: 69-75
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Significance of human beta-defensins in the epithelial lining fluid of patients with chronic lower respiratory tract infections. (2007)
  • Author(s): Yanagi S, Ashitani J, Imai K, Kyoraku Y, Sano A, Matsumoto N, Nakazato M
  • Journal Title: Clin Microbiol Infect 13 Pages: 63-69
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cardiovascular actions of central neuropeptide W in conscious rats. (2007)
  • Author(s): Yu N, Chu C, Kunitake T, Kato K, Nakazato M, Kannan H
  • Journal Title: Regul Pept 138 Pages: 82-86
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effects of intracerebroventricular administration of neuropeptide W30 on neurons in the hypothalamic paraventricular nucleus in the conscious rat. (2007)
  • Author(s): Yu NS, Kunitake T, Kato K, Nakazato M, Kannan H
  • Journal Title: Neurosci Lett 415 Pages: 140-145
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Long-term treatment with theophylline reduces neutrophils, interleukin-8 and tumor necrosis factor-a in the sputum of patients with chronic obstructive pulmonary disease. (2007)
  • Author(s): Iiboshi H, Ashitani J, Katoh S, Sano A, Matsumoto N, Mukae H, Nakazato M.
  • Journal Title: Pulm Pharmacol Ther 20 Pages: 46-51
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Elevated levels of antimicrobial peptides in bronchoalveolar lavage fluid in patients with chronic eosinophilic pneumonia. (2007)
  • Author(s): Ashitani J, Matsumoto N, Nakazato M.
  • Journal Title: Respiration 74 Pages: 69-75
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Significance of human beta-defensins in the epithelial lining fluid of patients with chronic lower respiratory tract infections. (2007)
  • Author(s): Yanagi S, Ashitani J, Imai K, Kyoraku Y, Sano A, Matsumoto N, Nakazato M.
  • Journal Title: Clin Microbiol Infect 13 Pages: 63-69
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Peripheral ghrelin transmits orexigenic signals through the noradrenergic pathway from the hindbrain to the hypothalamus. (2006)
  • Author(s): Date Y, Shimbara T, Koda S, Toshinai K, Ida T, Murakami N, Miyazato M, Kokame K, Ishizuka Y, Ishida Y, Kageyama H, Shioda S, Kangawa K, Nakazato M.
  • Journal Title: Cell Metab 4 Pages: 323-331
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Des-acyl ghrelin induces food intake by a mechanism independent of the growth hormone secretagogue receptor. (2006)
  • Author(s): Toshinai K, Yamaguchi H, Sun Y, Smith RG, Yamanaka A, Sakurai T, Date Y, Mondal MS, Shimbara T, Kawagoe T, Murakami N, Miyazato M, Kangawa K, Nakazato M
  • Journal Title: Endocrinology 147 Pages: 2306-2314
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Central effects of CRSP-1 on energy homeostasis in rats. (2006)
  • Author(s): Sawada H, Yamagucni H, Shimbara T, Toshinai K, Mondal MS, Date Y, Murakami N, Katafuchi T, Minamino N, Nunoi H, Nakazato M
  • Journal Title: Endocrinology 147 Pages: 2043-2050
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Neuronal pathway from the liver modulates energy expenditure and systemic insulin sensitivity. (2006)
  • Author(s): Uno K, Katagiri H, Yamada T, Ishigaki Y, Ogihara T, Imai J, Hasegawa Y, Gao J, Kaneko K, Iwasaki H, Ishihara H, Sasano H, Inukai K, Mizuguchi H, Asano T, Shiota M, Nakazato M, Oka Y
  • Journal Title: Science 312 Pages: 1656-1659
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Neuropeptide W is present in antral G cells of rat, mouse, and human stomach. (2006)
  • Author(s): Mondal MS, Kawagoe T, Yamaguchi H, Date Y, Kawagoe T, Tsuruta T, Kageyama H, Kawamura Y, Shioda S, Shimomura Y, Mori M, Nakazato M
  • Journal Title: J. Endocrinol 188 Pages: 49-57
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Isolation of human β-defensin-4 in lung tissue and its increase in lower respiratory tract infection. (2006)
  • Author(s): Yanagi S, Ashitani J, Ishimoto H, Date Y, Mukae H, Chino N, Nakazato M
  • Journal Title: Respir Res 6 Pages: 130
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Ghrelin stimulates growth hormone secretion and food intake in aged rats. (2006)
  • Author(s): Toshinai K, Mondal MS, Shimbara T, Yamaguchi H, Date Y, Kangawa K, Nakazato M
  • Journal Title: Mech Ageing Dev
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Plasma leptin and ghrelin concentrations in patients with Crohn's disease. (2006)
  • Author(s): Nishi Y, Isomoto H, Ueno H, Ohnita K, Wen CY, Takeshima F, Mishima R, Nakazato M, Kohno S
  • Journal Title: World J Gastroenterol 11 Pages: 7314-7317
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Galanin-like peptide promotes feeding behavior via activation of orexinergic neurons in the rat lateral hypothalamus. (2006)
  • Author(s): Kageyama H, Kita T, Toshinai K, Guan JL, Date Y, Takenoya F, Kato S, Ohtaki T, Matsumoto H, Nakazato M, Shioda S
  • Journal Title: J. Neuroendocrinol 18 Pages: 33-41
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Maternal ghrelin an important role in rat fetal development during pregnancy. (2006)
  • Author(s): Nakahara K, Nakagawa M, Baba Y, Sato M, Toshinai K, Date Y, Nakazato M, Kojima M, Miyazato M, Kaiya H, Hosoda H, Kangawa K, Murakami N
  • Journal Title: Endocrinology 147 Pages: 1333-1342
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Postnatal development of orexin-A and orexin-B like immunoreactivities in the eastern grey kangaroo (macropus giganteus) hypothalamus. (2006)
  • Author(s): Yamamoto Y, Mckinley MJ, Nakazato M, Yamashita H, Shirahata A, Ueta Y
  • Journal Title: Neurosci Lett 392 Pages: 124-128
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of hBD-3 in respiratory tract and serum and its increase in pneumonia. (2006)
  • Author(s): Ishimoto H, Mukae H, Date Y, Shimbara T, Mondal MS, Ashitani J, Hiratsuka T, Kubo S, Kohno S, Nakazato M
  • Journal Title: Eur Respir J 27 Pages: 253-260
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Fasting-induced reduction in locomotor activity and reduced response of orexin neurons in carnitine-deficient mice. (2006)
  • Author(s): Yoshida G, Li MX, Horjuchi M, Nakagawa S, Sakata M, Kuchiiwa S, Kuchiiwa T, Jalil MA, Begum L, Lu YB, Iijima M, Hanada T, Nakazato M, Huang ZL, Eguchi N, Kobayashi K, Saheki T
  • Journal Title: Neurosci Res 55 Pages: 78-86
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Chemical Synthesis of human β-defensin -1, -2, -3 and -4 : optimization of the oxidative folding reaction. (2006)
  • Author(s): Chino N, Kubo S, Nishio H, Nishiuchi Y, Nakazato M, Kimura T
  • Journal Title: Int J Pept Res Therapeutics 12 Pages: 203-209
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Ghrelin concentrations and cardiac vagal tone are decreased after pharmacologic and cognitive-behavioral treatment in patients with bulimia nervosa. (2006)
  • Author(s): Tanaka M, Nakahara T, Muranaga T, Kojima S, Yasuhara D, Ueno H, Nakazato M, Inui A
  • Journal Title: Horm Behav 50 Pages: 261-265
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Centrally administered neuropeptide W-30 activates magnocellular neurosecretory cells in the supraoptic and paraventricular nuclei with neurosecretion in rats. (2006)
  • Author(s): Kawasaki M, Onaka T, Nakazato M, Saito J, Mera T, Hashimoto H, Fujihara H, Okimoto N, Ohnishi H, Nakamura T, Ueta Y
  • Journal Title: J Endocrinol 190 Pages: 213-223
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Changes in plasma ghrelin levels, gastric ghrelin production, and body weight after Helicobacter pylori cure. (2006)
  • Author(s): Osawa H, Kita H, Ohnishi H, Nakazato M, Date Y, Bowlus CL, Ishino Y, Watanabe E, Shiiya T, Ueno H, Hoshino H, Satoh K, Sugano K
  • Journal Title: J Gastroenterol 41 Pages: 954-961
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effects of ghrelin and des-acyl ghrelin on neurogenesis of the rat fetal spinal cord. (2006)
  • Author(s): Sato M, Nakahara K, Goto S, Kaiya H, Miyazato M, Date Y, Nakazato M, Kangawa K, Murakami N
  • Journal Title: Biochem Biophys Res Commun 350 Pages: 598-603
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Plasma leptin and ghrelin concentrations in patients with Crohn's disease. (2006)
  • Author(s): Nishi Y, Isomoto H, Ueno H, Ohnita K, Wen CY, Takeshima F, Mishima R, Nakazato M, Kohno S.
  • Journal Title: World J Gastroenterol 11 Pages: 7314-7317
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Galanin-like peptide promotes feeding behavior via activation of orexinergic neurons in the rat lateral hypothalamus. (2006)
  • Author(s): Kageyama H, Kita T, Toshinai K, Guan JL, Date Y, Takenoya F, Kato S, Ohtaki T, Matsumoto H, Nakazato M, Shioda S.
  • Journal Title: J Neuroendocrinol 18 Pages: 33-41
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Maternal ghrelin plays an important role in rat fetal development during pregnancy. (2006)
  • Author(s): Nakahara K, Nakagawa M, Baba Y, Sato M, Toshinai K, Date Y, Nakazato M, Kojima M, Miyazato M, Kaiya H, Hosoda H, Kangawa K, Murakami N.
  • Journal Title: Endocrinology 147 Pages: 1333-1342
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Neuropeptide W is present in antral G cells of rat, mouse, and human stomach. (2006)
  • Author(s): Mondal MS, Kawagoe T, Yamaguchi H, Date Y, Kawagoe T, Tsuruta T, Kageyama H, Kawamura Y, Shioda S, Shimomura Y, Mori M, Nakazato M.
  • Journal Title: J Endocrinol 188 Pages: 49-57
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Postnatal development of orexin-A and orexin-B like immunoreactivities in the eastern grey kangaroo hypothalamus. (2006)
  • Author(s): Yamamoto Y, Mckinley MJ, Nakazato M, Yamashita H, Shirahata A, Ueta Y.
  • Journal Title: Neurosci Lett 392 Pages: 124-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prognostic value of cardiac 1-123 metaiodobenzylguanidine imaging in patients with non-insulin-dependent diabetes mellitus. (2006)
  • Author(s): Nagamachi S, Fujita S, Nishii R, Futami S, Tamura S, Mizuta M, Na kazato M, Kurose T, Wakamatsu H.
  • Journal Title: J Nucl Cardiol 13 Pages: 34-42
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of hBD-3 in respiratory tract and serum and its increase in pneumonia. (2006)
  • Author(s): Ishimoto H, Mukae H, Date Y, Shimbara T, Mondal MS, Ashitani J, Hiratsuka T, Kubo S, Kohno S, Nakazato M.
  • Journal Title: Eur Respir J 27 Pages: 253-260
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation of human B-defensin-4 in lung tissue and its increase in lower respiratory tract infection. (2006)
  • Author(s): Yanagi S, Ashitani J, Ishimoto H, Date Y, Mukae H, Chino N, Nakazato M.
  • Journal Title: Respir Res 6 Pages: 130
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Fasting-induced reduction in locomotor activity and reduced response of orexin neurons in carnitine-deficient mice. (2006)
  • Author(s): Yoshida G, Li MX, Horiuchi M, Nakagawa S, Sakata M, Kuchiiwa S, Kuchiiwa T, Jalil MA, Begum L, Lu YB, Iijima M, Hanada T, Nakazato M, Huang ZL, Eguchi N, Kobayashi K, Saheki T.
  • Journal Title: Neurosci Res 55 Pages: 78-86
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Des-acyl ghrelin induces food intake by a mechanism independent of the growth hormone secretagogue receptor. (2006)
  • Author(s): Toshinai K, Yamaguchi H, Sun Y, Smith RG, Yamanaka A, Sakurai T, Date Y, Mondal MS, Shimbara T, Kawagoe T, Murakami N, Miyazato M, Kangawa K, Nakazato M.
  • Journal Title: Endocrinology 147 Pages: 2306-2314
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Central effects of CRSP-1 on energy homeostasis in rats. (2006)
  • Author(s): Sawada H, Yamaguchi H, Shimbara T, Toshinai K, Mondal MS, Date Y, Murakami N, Katafuchi T, Minamino N, Nunoi H, Nakazato M.
  • Journal Title: Endocrinology 147 Pages: 2043-2050
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Neuronal pathway from the liver modulates energy expenditure and systemic insulin sensitivity. (2006)
  • Author(s): Uno K, Katagiri H, Yamada T, Ishigaki Y, Ogihara T, Imai J, Hasegawa Y, Gao J, Kaneko K, Iwasaki H, Ishihara H, Sasano H, Inukai K, Mizuguchi H, Asano T, Shiota M, Nakazato M, Oka Y.
  • Journal Title: Science 312 Pages: 1656-1659
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Chemical Synthesis of human β-defensin-1,-2, -3 and -4 : optimization of the oxidative folding reaction. (2006)
  • Author(s): Chino N, Kubo S, Nishio H, Nishiuchi Y, Nakazato M, Kimura T.
  • Journal Title: Int J Pept Res Therapeutics 12 Pages: 203-209
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ghrelin concentrations and cardiac vagal tone are decreased after pharmacologic and cognitive-behavioral treatment in patients with bulimia nervosa. (2006)
  • Author(s): Tanaka M, Nakahara T, Muranaga T, Kojima S, Yasuhara D, Ueno H, Nakazato M, Inui A.
  • Journal Title: Horm Behav 50 Pages: 261-265
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Centrally administered neuropeptide W-30 activates magnocellular neurosecretory cells in the supraoptic and paraventricular nuclei with neurosecretion in rats. (2006)
  • Author(s): Kawasaki M, Onaka T, Nakazato M, Saito J, Mera T, Hashimoto H, Fujihara H, Okimoto N, Ohnishi H, Nakamura T, Ueta Y.
  • Journal Title: J Endocrinol 190 Pages: 213-223
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Elevated BALF concentrations of alpha-and beta-defensins in patients with pulmonary alveolar proteinosis. (2006)
  • Author(s): Mukae H, Ishimoto H, Yanagi S, Ishii H, Nakayama S, Ashitani JI, Nakazato M, Kohno S.
  • Journal Title: Respir Med.
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cardiovascular actions of central neuropeptide W in conscious rats. (2006)
  • Author(s): Yu N, Chu C, Kunitake T, Kato K, Nakazato M, Kannan H.
  • Journal Title: Regul Pept.
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Incomplete restoration of the secretion of ghrelin and PYY compared to insulin after food ingestion following weight gain in anorexia nervosa. (2006)
  • Author(s): Nakahara T, Kojima S, Tanaka M, Yasuhara D, Harada T, Sagiyama KI, Muranaga T, Nagai N, Nakazato M, Nozoe SI, Naruo T, Inui A.
  • Journal Title: J Psychiatr Res.
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Changes in plasma ghrelin levels, gastric ghrelin production, and body weight after Helicobacter pylori cure. (2006)
  • Author(s): Osawa H, Kita H, Ohnishi H, Nakazato M, Date Y, Bowlus CL, Ishino Y, Watanabe E, Shiiya T, Ueno H, Hoshino H, Satoh K, Sugano K.
  • Journal Title: J Gastroenterol 41 Pages: 954-961
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of ghrelin and des-acyl ghrelin on neurogenesis of the rat fetal spinal cord. (2006)
  • Author(s): Sato M, Nakahara K, Goto S, Kaiya H, Miyazato M, Date Y, Nakazato M, Kangawa K, Murakami N.
  • Journal Title: Biochem Biophys Res Commun 350 Pages: 598-603
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of ghrelin neurons and its receptor, GHS-R in rat brain. (2005)
  • Author(s): Mondal MS, Date Y, Yamaguchi H, Toshinai K, Kangawa K, Nakazato M
  • Journal Title: Regul Pept 126 Pages: 55-59
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Ghrelin : A gastric peptide that regulates food intake and energy homeostasis. (2005)
  • Author(s): Ueno H, Yamaguchi H, Nakazato M
  • Journal Title: Regul Pept 126 Pages: 11-19
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The role of the vagal nerve in peripheral PYY3-36-induced feeding reduction in rats. (2005)
  • Author(s): Koda S, Date Y, Murakami N, Shimbara T, Hanada T, Toshinai K, Niiji A, Furuya M, Inomata N, Osuye K, Nakazato M
  • Journal Title: Endocrinology 146 Pages: 2369-2375
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Peripheral interaction of ghrelin with cholecystokinin on feeding regulation. (2005)
  • Author(s): Date Y, Toshinai K, Koda S, Miyazato M, Shimbara T, Tsuruta T, Niijima A, Kangawa K, Nakazato M
  • Journal Title: Endocrinology 146 Pages: 3518-3525
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Morphological analysis of ghrelin and its receptor distribution in the rat pancreas (2005)
  • Author(s): Kageyama H, Funahashi H, Hirayama M, Takenoya F, Kita T, Kato S, Sakurai J, Lee EY, Inoue S, Date Y, Nakazato M, Kangawa K, Shioda S
  • Journal Title: Regul Pept 126 Pages: 67-71
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Impaired production of gastric ghrelin combined with decreased plasma ghrelin in chronic gastritis associated with helicobacter pylori. (2005)
  • Author(s): Osawa H, Nakazato M, Date Y, Kita H, Ohnishi H, Ueno H, Shiiya T, Sato K, Ishino Y, Sugano K
  • Journal Title: J Clin Endocrinol Metab 90 Pages: 10-16
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Altered ghrelin and peptide YY responses to meals in bulimia nervosa. (2005)
  • Author(s): Kojima S, Nakahara T, Nagai N, Muranaga T, Tanaka M, Yasuhara D, Masuda A, Date Y, Ueno H, Nakazato M, Naruo T
  • Journal Title: Clin Endocrinol 62 Pages: 74-78
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Concentrations of alpha- and beta-defensins in gastric juice of patients with various gastroduodenal diseases. (2005)
  • Author(s): Nishi Y, Isomoto H, Mukae H, Ishimoto H, Wen CY, Wada A, Ohnita K, Mizuta Y, Murata I, Hirayama T, Nakazato M, Kohno S.
  • Journal Title: World J Gastroenterol 11 Pages: 99-103
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Impact of helicobacter pylori infection on ghrelin and various neuroendocrine hormones in plasma. (2005)
  • Author(s): Isomoto H, Ueno H, Nishi Y, Wen CY, Nakazato M, Kohno S
  • Journal Title: World J Gastroenterol 11 Pages: 1644-1648
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differential effects of alpha- and beta-defensin on cytokine production by cultured human bronchial epithelial cells. (2005)
  • Author(s): Sakamoto N, Mukae H, Fujii T., Ishii H, Yoshioka S, Kakugawa T, Sugiyama K, Mizuta Y, Kadota JI, Nakazato M, Kohno S
  • Journal Title: Am J Physiol Lung Cell Mol Physiol 288 Pages: L508-L513
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Circulating ghrelin levels in patients with various upper gastrointestinal diseases. (2005)
  • Author(s): Isomoto H, Ueno H, Nishi Y, Yasutake T, Tanaka K, Kawano N, Ohnita K, Mizuta Y, Inoue K, Nakazato M, Kohno S
  • Journal Title: Dig Dis Sci 50 Pages: 833-838
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The relationship between plasma and gastric ghrelin levels and strain diversity in helicobacter pylori virulence. (2005)
  • Author(s): Isomoto H, Nishi Y, Ohnita K, Mizuta Y, Kohno S, Ueno H, Nakazato M
  • Journal Title: Am J Gastroenterol 100 Pages: 1425-1427
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High concentrations of human beta-defensin 2 in gastric juice of patients with Helicobacter pylori infection. (2005)
  • Author(s): Isomoto H, Mukae H, Ishimoto H, Nishi Y, Wen CY, Wada A, Ohnita K, Hirayama T, Nakazato M, Kohno S
  • Journal Title: World J Gastroenterol 11 Pages: 4782-4787
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Impact of Helicobacter pylori infection on gastric and plasma ghrelin dynamics in humans. (2005)
  • Author(s): Isomoto H, Ueno H, Saenko VA, Mondal MS, Nishi Y, Kawano N, Ohnita K, Mizuta Y, Ohtsuru A, Yamashita S, Nakazato M, Kohno S
  • Journal Title: Am J Gastroenterol 100 Pages: 1711-1720
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Repeated thermal therapy enhances appetite and subjective sleep quality in mildly depressed patients. (2005)
  • Author(s): Masuda A, Nakazato M, Kihara T, Minagoe S, Tei C
  • Journal Title: Psychosom Med 67 Pages: 643-647
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Impaired production of gastric ghrelin combined with decreased plasma ghrelin in chronic gastritis associated with helicobacter pylori. (2005)
  • Author(s): Osawa H, Nakazato M, Date Y, Kita H, Ohnishi H, Ueno H, Shiiya T, Sato K, Ishino Y, Sugano K.
  • Journal Title: J Clin Endocrinol Metab 90 Pages: 10-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Morphological analysis of ghrelin and its receptor distribution in the rat pancreas. (2005)
  • Author(s): Kageyama H, Funahashi H, Hirayama M, Takenoya F, Kita T, Kato S, Sakurai J, Lee EY, Inoue S, Date Y, Nakazato M, Kangawa K, Shioda S.
  • Journal Title: Regul Pept 126 Pages: 67-71
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of ghrelin neurons and its receptor, GHS-R, in rat brain. (2005)
  • Author(s): Mondal MS, Date Y, Yamaguchi H, Toshinai K, Kangawa K, Nakazato M
  • Journal Title: Regul Pept 126 Pages: 55-59
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ghrelin : A gastric peptide that regulates food intake and energy homeostasis. (2005)
  • Author(s): Ueno H, Yamaguchi H, Nakazato M.
  • Journal Title: Regul Pept 126 Pages: 11-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Concentrations of alpha-and beta-defensins in gastric juice of patients with various gastroduodenal diseases. (2005)
  • Author(s): Nishi Y, Isomoto H, Mukae H, Ishimoto H, Wen CY, Wada A, Ohnita K, Mizuta Y, Murata I, Hirayama T, Nakazato M, Kohno S.
  • Journal Title: World J Gastroenterol 11 Pages: 99-103
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of uroguanylin, a peptide with natriuretic activity, in rats with experimental nephritic syndrome. (2005)
  • Author(s): Kikuchi M, Fujimoto S, Fukae H, Kinoshita H, Kita T, Nakazato M, Eto T.
  • Journal Title: J Am Soc Nephrol 16 Pages: 392-397
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Impact of helicobacter pylori infection on ghrelin and various neuroendocrine hormones in plasma. (2005)
  • Author(s): Isomoto H, Ueno H, Nishi Y, Wen CY, Nakazato M, Kohno S.
  • Journal Title: World J Gastroenterol 11 Pages: 1644-1648
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential effects of alpha-and beta-defensin on cytokine production by cultured human bronchial epithelial cells. (2005)
  • Author(s): Sakamoto N, Mukae H, Fujii T, Ishii H, Yoshioka S, Kakugawa T, Sugiyama K, Mizuta Y, Kadota JI, Nakazato M, Kohno S
  • Journal Title: Am J Physiol Lung Cell Mol Physiol 288 Pages: L508-L513
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The role of the vagal nerve in peripheral PYY3-36-induced feeding reduction in rats. (2005)
  • Author(s): Koda S, Date Y, Murakami N, Shimbara T, Hanada T, Toshinai K, Niiji A, Furuya M, Inomata N, Osuye K, Nakazato M.
  • Journal Title: Endocrinology 146 Pages: 2369-2375
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Clinical and electrophysiologic correlates of IVIg responsiveness in CIDP. (2005)
  • Author(s): Iijima M, Yamamoto M, Hirayama M, Tanaka F, Katsuno M, Mori K, Koike H, Hattori N, Arimura K, Nakagawa M, Yoshikawa H, Hayasaka K, Onodera O, Baba M, Yasuda H, Saito T, Nakazato M, Nakashima K, Kira J, Kaji R, Oka N, Sobue G.
  • Journal Title: Neurology 64 Pages: 1471-1475
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Circulating ghrelin levels in patients with various upper gastrointestinal diseases. (2005)
  • Author(s): Isomoto H, Ueno H, Nishi Y, Yasutake T, Tanaka K, Kawano N, Ohnita K, Mizuta Y, Inoue K, Nakazato M, Kohno S.
  • Journal Title: Dig Dis Sci 50 Pages: 833-838
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The relationship between plasma and gastric ghrelin levels and strain diversity in helicobacter pylori virulence. (2005)
  • Author(s): Isomoto H, Nisai Y, Ohnita K, Mizuta Y, Kohno S, Ueno H, Nakazato M.
  • Journal Title: Am J Gastroenterol 100 Pages: 1425-1427
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Peripheral interaction of ghrelin with cholecystokinin on feeding regulation. (2005)
  • Author(s): Date Y, Toshinai K, Koda S, Miyazato M, Shimbara T, Tsuruta T, Niijima A, Kangawa K, Nakazato M.
  • Journal Title: Endocrinology 146 Pages: 3518-3525
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Age associated axonal features in HNPP with 17p11.2 deletion in Japan. (2005)
  • Author(s): Koike H, Hirayama M, Yamamoto M, Ito H, Hattori N, Umehara F, Arimura K, Ikeda S, Ando Y, Nakazato M, Kaji R, Hayasaka K, Nakagawa M, Sakoda S, Matsumura K, Onodera 0, Baba M, Yasuda H, Saito T, Kira J, Nakashima K, Oka N, Sobue G.
  • Journal Title: J Neurol Neurosurg Psychiatry 76 Pages: 1109-1114
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High concentrations of human beta-defensin 2 in gastric juice of patients with Helicobacter pylori infection. (2005)
  • Author(s): Isomoto H, Mukae H, Ishimoto H, Nishi Y, Wen CY, Wada A, Ohnita K, Hirayama T, Nakazato M, Kohno S.
  • Journal Title: World J Gastroenterol 11 Pages: 4782-4787
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Impact of Helicobacter pylori infection on gastric and plasma ghrelin dynamics in humans. (2005)
  • Author(s): Isomoto H, Ueno H, Saenko VA, Mondal MS, Nishi Y, Kawano N, Ohnita K, Mizuta Y, Ohtsuru A, Yamashita S, Nakazato M, Kohno S.
  • Journal Title: Am J Gastroenterol 100 Pages: 1711-1720
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Repeated thermal therapy enhances appetite and subjective sleep quality in mildly depressed patients. (2005)
  • Author(s): Masuda A, Nakazato M, Kihara T, Minagoe S, Tei C.
  • Journal Title: Psychosom Med 67 Pages: 643-647
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Upregulation of ghrelin expression in cachectic nude mice bearing Human melanoma cells. (2004)
  • Author(s): Hanada T, Toshinai K, Date Y, Kajimura N, Tsukada T, Hayashi Y, Kangawa K, Nakazato M
  • Journal Title: Metabolism 53 Pages: 84-88
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Central stresscopin modulates cardiovascular function through the adrenal medulla in conscious rats. (2004)
  • Author(s): Chu CP, Qiu DL, Kato K, Kunitake T, Watanabe S, Yu NS, Nakazato M, Kannan H
  • Journal Title: Regul Pept 119 Pages: 53-59
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High concentrations of a-defensins in plasma and bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome. (2004)
  • Author(s): Ashitani J, Mukae H, Arimura Y, Sano A, Tokojima M, Nakazato M
  • Journal Title: Life Sci 75 Pages: 1123-1134
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Low plasma ghrelin levels in patients with Helicobacter pylori-associated gastritis. (2004)
  • Author(s): Isomoto H, Nakazato M, Ueno H, Date Y, Nishi Y, Mukae H, Mizuta Y, Ohtsuru A, Yamashita S, Kohno S
  • Journal Title: Am J Med 117 Pages: 429-432
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Central administration of ghrelin preferentially enhances fat ingestion (2004)
  • Author(s): Shimbara T, Mondal MS, Kawagoe T, Toshinai K, Koda S, Yamaguchi H, Date Y, Nakazato M
  • Journal Title: Neurosci Lett 369 Pages: 75-79
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Elevated concentrations of a-defensins in gastric juice of patients with helicobacter pylori infection. (2004)
  • Author(s): Isomoto H, Mukae H, Ishimoto H, Date Y, Nishi Y, Inoue K, Wada A, Hirayama T, Nakazato M., Kohno S
  • Journal Title: Am J Gastroenterol 99 Pages: 1916-1923
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effect of nutritional rehavioitation of circulating ghrelin and growth hormones levels in patients with anorexia nervosa. (2004)
  • Author(s): Tanaka M, Nakahara T, Kojima S, Nakao T, Muranaga T, Nagai N, Ueno H, Nakazato M, Nozoe S, Naruo T
  • Journal Title: Regul Pept 122 Pages: 163-168
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Elevated serum beta-defensins concentrations in patients with lung cancer. (2004)
  • Author(s): Arimura Y, Ashitani J, Yanagi S, Tokojima M, Abe K, Mukae H, Nakazato M.
  • Journal Title: Anticancer Res 24 Pages: 4051-4057
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Upregulation of ghrelin expression in cachectic nude mice bearing human melanoma cells. (2004)
  • Author(s): Hanada T, Toshinai K, Date Y, Kajimura N, Tsukada T, Hayashi Y, Kangawa K, Nakazato M
  • Journal Title: Metabolism 53 Pages: 84-88
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Central stresscopin modulates cardiovascular function through the adrenal medulla in conscious rats. (2004)
  • Author(s): Chu CP, Qiu DL, Kato K, Kunitake T, Watanabe S, Yu NS, Nakazato M, Kannan H.
  • Journal Title: Regul Pept 119 Pages: 53-59
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High concentrations of a-defensins in plasma and bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome. (2004)
  • Author(s): Ashitani J, Mukae H, Arimura Y, Sano A, Tokojima M, Nakazato M.
  • Journal Title: Life Sci 75 Pages: 1123-1134
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of dexamethasone and cyclosporin A on human beta-defensin in corneal epithelial cells. (2004)
  • Author(s): Terai K, Sano Y, Kawasaki S, Endo K, Adachi W, Hiratsuka T, Ihiboshi H, Nakazato M, Kinoshita S.
  • Journal Title: Exp Eye Res 79 Pages: 175-180
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Low plasma ghrelin levels in patients with Helicobacter poylori associated gastritis. (2004)
  • Author(s): Isomoto H, Nakazato M, Ueno H, Date Y, Nishi Y, Mukae H, Mizuta Y, Ohtsuru A, Yamashita S, Kohno S.
  • Journal Title: Am J Med 117 Pages: 429-432
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Central administration of ghrelin preferentially enhances fat ingestion. (2004)
  • Author(s): Shimbara T, Mondal MS, Kawagoe T, Toshinai K, Koda S, Yamaguchi H, Date Y, Nakazato M.
  • Journal Title: Neurosci Lett 369 Pages: 75-79
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Familial leptomeningeal amyloidosis with a transthyretin variant Asp 18Gly representing repeated subarachnoid haemorrhages with superficial siderosis. (2004)
  • Author(s): Jin K, Sato S, Takahashi T, Nakazaki H, Date Y, Nakazato M, Tominaga T, Itoyama Y, Ikeda S.
  • Journal Title: J Neurol Neurosurg Psychiatry 75 Pages: 1463-1466
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Elevated concentrations of a-defensins in gastric juice of patients with helicobacter pylori infection. (2004)
  • Author(s): Isomoto H, Mukae H, Ishimoto H, Date Y, Nishi Y, Inoue K, Wada A, Hirayama T, Nakazato M, Kohno S.
  • Journal Title: Am J Gastroenterol 99 Pages: 1916-1923
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of nutritional rehavioitation of circulating ghrelin and growth hormones levels in patients with anorexia nervosa. (2004)
  • Author(s): Tanaka M, Nakahara T, Kojima S, Nakao T, Muranaga T, Nagai N, Ueno H, Nakazato M, Nozoe S, Naruo T.
  • Journal Title: Regul Pept 122 Pages: 163-168
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Elevated BALF concentrations of alpha- and beta-defensins in patients with pulmonary alveolar proteinosis.
  • Author(s): Mukae H, Ishimoto H, Yanagi S, Ishii H, Nakayama S, Ashitani JI, Nakazato M, Kohno S
  • Journal Title: Respir Med (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Incomplete restoration of the secretion of ghrelin and PYY compared to insulin after food ingestion following weight gain in anorexia nervosa.
  • Author(s): Nakahara T, Kojima S, Tanaka M, Yasuhara D, Harada T, Sagiyama KI, Muranaga T, Nagai N, Nakazato M, Nozoe SI, Naruo T, Inui A
  • Journal Title: J Psychiatr Res (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Ontogeny of a New Enteric Peptide, Neuropeptide W (NPW), in the Developing Rat Stomach.
  • Author(s): Mondal MS, Yamaguchi H, Date Y, Tsuruta T, Shimbara T, Toshinai K, Shimomura Y, Mori M, Nakazato M
  • Journal Title: Regul Pept (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The role of PYY3-36 in the feeding regulation.
  • Author(s): Ueno H, Yamaguchi H, Mizuta M, Nakazato M
  • Journal Title: Regul Pept (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Ontogeny of a New Enteric Peptide, Neuropeptide W (NPW), in the Developing Rat Stomach.
  • Author(s): Mondal MS, Yamaguchi H, Date Y, Tsuruta T, Shimbara T, Toshinai K, Shimomura Y, Mori M, Nakazato M.
  • Journal Title: Regul Pept. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The role of PYY3-36 in the feeding regulation.
  • Author(s): Ueno H, Yamaguchi H, Mizuta M, Nakazato M.
  • Journal Title: Regul Pept. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] メタボリックシンドロームメカニズムと治療の分子生物学 (2005)
  • Author(s): 上野浩晶, 中里雅光
  • Total Pages: 176
  • Publisher: 南山堂(東京)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] The Orexin/Hypocretin System : Physiology And Pathophysiology (2005)
  • Author(s): Shirasaka T, Nakazato M
  • Total Pages: 397
  • Publisher: Humana Press, Totowa, NJ USA
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 新規ポリペプチドおよびその用途
  • Inventor(s): 中里雅光, 高尾敏文
  • Industrial Property Rights Holder: 宮崎大学, 大阪大学蛋白研究所, 武田薬品工業株式会社
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 摂食調節または摂水障害の治療薬
  • Inventor(s): 中里雅光, 山崎基生
  • Industrial Property Rights Holder: 宮崎大学, 協和発酵工業株式会社
  • Description: 「研究成果報告書概要(和文)」より