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2006 Fiscal Year Final Research Report Summary

Novel methods for the induction on the differentiation of human amniotic stem cell to the pancreatic islet cells.

Research Project

Project/Area Number 16390384
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionJikei University

Principal Investigator

ISHIKAWA Hiroshi  Jikei University, School of Medicine, Professor, 医学部, 教授 (30089784)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Hisashi  Jikei University, School of Medicine, Associate Professor, 医学部, 助教授 (80189498)
TACHIBANA Toshiaki  Jikei University, School of Medicine, Lecture, 医学部, 講師 (80163476)
OHI Satoshi  Jikei University, School of Medicine, Research Assistant, 医学部, 助手 (80385301)
Project Period (FY) 2004 – 2006
Keywordshuman amnion / bio-artificial islets / induction of the differentiation / tissue stem cell / embryotrophic factors / embryonic monster / embryoid body / hanging drop method
Research Abstract

The HAM-R1,HAM-R2 and HAM-R3 cell lines were successfully established from human amniotic stem cell isolated by FACS from amnion of fullterm placenta. Embryoid body was differentiated from above cell line (HAM-R1) by hanging drop method using embryotrophic factors (ETFs). Then, embryonic monster was developed from embryoid body cultured with ETFs-supplemented medium by circumfusion apparatus. Average of the development of embryonic monster from the embryoid body was about 2%. The anlage of the pancreatic islets was eviscerated from the embryonic monster. The single cells were obtained from the eviscerated anlage by dissociation with 0.1%trypsin-0.02%EDTA/PBS(-) and were cultured in DMEM/F12 medium supplemented 15%FBS. Insulin secreted primary cultures were selected and cultured with above growth medium. For openers, the amount of insulin secretion was increased dose dependently on the amount of glucose in the medium. However the amount of insulin secretion was decreased gradually by the passage at 6th. At 12 passages, the insulin secretion was not observed. Whereas, the cells were put into the collagen sponge by syringe for the reconstruction of islets. The reconstructed islet was secreted insulin dose dependently to the glucose during 40 days, then insulin secretion was decreased.

  • Research Products

    (8 results)

All 2006 Other

All Journal Article (8 results)

  • [Journal Article] ヒト羊膜由来幹細胞系HAMの樹立と特性2006

    • Author(s)
      石渡勇, 石川博 他
    • Journal Title

      日本再生医療学会雑誌 Vol.5(suppl)

      Pages: 189

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] マウスearly ES細胞の甲状腺原基ヘの分化・発育2006

    • Author(s)
      石川真由美, 大井聡, 立花利公他
    • Journal Title

      日本再生医療学会雑誌 Vol.5(suppl)

      Pages: 118

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Establishment and characteristic of the amniotic stem cell line (HAM) derived from human amnion of the fullterm placenta.2006

    • Author(s)
      Ishiwata I, Ishikawa H, et al.
    • Journal Title

      Regenerative Medicine 5

      Pages: 189

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development and differentiation of the mouse early ES cell to the anlage of the thyroid.2006

    • Author(s)
      Ishikawa M, Ohi S, Tachibana T, et al.
    • Journal Title

      Regenerative Medicine 5

      Pages: 118

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Human amniotic cells, HEA-1 cells, differentiated ino nerve or glial cells with retinoic acid and FTFs medium.

    • Author(s)
      Hashimoto H, Ishikawa H, et al.
    • Journal Title

      Human Cell (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Human amnion messenchymal cells possess hepatocyte-like characteristic in vitro.

    • Author(s)
      Tamagawa T, Ishiwata I, Nakamura Y, Ohi S, Ishikawa H
    • Journal Title

      Human Cell (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Human amniotic cells, HEA-1 cells, differentiated ino nerve or glial cells with retinoic acid and FTFs medium.

    • Author(s)
      Hashimoto H, Ishikawa H, et al.
    • Journal Title

      Human Cell (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ishikawa H Human amnion messenchymal cells possess hepatocyte-like characteristic in vitro.

    • Author(s)
      Tamagawa T, Ishiwata I, Nakamura Y, Ohi S
    • Journal Title

      Human Cell (in press)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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