2005 Fiscal Year Final Research Report Summary
Analysis of the function of TM4SF complex and its application of lung cancer therapy.
| Project/Area Number |
16390400
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | The Tazuke Kofukai |
|---|
Principal Investigator |
MIYAKE Masayuki The Tazuke Kofukai, Kitano Hospital The Tazuke Kofukai Medical Research Institute. The Fifth Department of Oncology, Department of Thoracic Surgery, Director, Associate-Director, 医学研究所第5研究部, 部長 (90250076)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HATTORI Noboru Hiroshima University, Graduate School of Biomedical Sciences, Department of Molecular and Internal Medicine, Associate Professor, 大学院・医歯薬学総合研究科第2内科, 講師 (00283169)
HIROTA Kiichi Kyoto University, Graduate School of Medicine, Department of Anesthesia, Associate Professor, 医学研究科麻酔科, 講師 (00283606)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Keywords | TM4SF / AAH / MRP-1 / CD9 / KAI1 / CD82 / PETA3 / CD151 |
|---|
| Research Abstract |
Previously, we found that MRP-1/CD9, a member of the tetraspanin family, suppressed cell motility and metastatic potential to lungs. Moreover, reduction of MRP-1/CD9 gene expression was found to be a factor of a poor prognosis for the patients with non-small cell lung cancer, as was another member of TM4SF, KAI1/CD82. On the other hand, CD151 is identical to an exciting gene PETA-3 which may promote tumor metastasis of malignant cells and its expression may be involved in the malignant progression cancer. We have established various stages of lung cancer cell lines and investigated the complex between tetraspsnin family members and integrin family members. Especially, the complex formation of MRP-1/CD9, KAI1/CD82 and PETA3/CD151 were analyzed. In the Stage I, MRP-1/CD9 and KAI1/CD82 expression were found in two cell lines except one, but PETA3/CD151 appeared weak expression. In the Stage II, KAI1/CD82 was decreasing in 5 cases and MRP-1/CD9 was decreasing in 1 case, however PETA3/CD151 was increasing in 2 cases. In the StageIII, only KAI1/CD82was found decreasing in 4 cases. Then only PETA3/CD151 was appeared in the last 1case. In the StageIV, expression of PETA3/CD151 was positive in almost all metastatic cell lines. From these experiments, MRP-1/CD9 and KAI1/CD82 play an important role as metastasis suppressor genes. On the other hand, PETA3/CD151 plays a metastasis promotion gene. Hence, the balance and unbalance of these expressions have something to do with the process of 'metastasis. Besides, we have found that MRP-1/CD9 and KAI1/CD82 regulate VEGF-A gene and Wnt-1 gene. In addition, we have established the monoclonal antibody recognizing MRP-1/CD9-integrin α3 complex. Using this antibody, we will be able to analyze the mechanism of this complex.
|
Research Products
(24 results)
-
-
-
-
-
-
-
[Journal Article] Circulating aminopeptidase N/CD13 is an independent prognostic factor in patients with non-small cell lung cancer2005
Author(s)
Murakami, H., Yokoyama, A., Kondo, K., Nakanishi, S., Kohno, N., Miyake, M.
-
Journal Title
Clin.Cancer Res 11
Pages: 8674-8679
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] KL-6, a human NUC1 mucin, promotes proliferation and survival of Lung fibroblasts.2005
Author(s)
Ohshimo, S., Yokoyama, A., Hattori, N., Ishikawa, N., Hirasawa, Y., Kohno, N.
-
Journal Title
Biochem.Biophys.Res.Commun 30
Pages: 1845-1852
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
[Journal Article] The Plasminogen activation system reduces fibrosis in the lung by a Hepatocyte growth factor-dependent mechanism.2004
Author(s)
Hattori, N., Mizuno, S., Yoshida, Y., Chin, K., Mishima, M., Sisson, TH., Simon, RH., Nakamura, T., Miyake, M.
-
Journal Title
Am.J.Pathol. 164
Pages: 1091-1098
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] MRP-1/CD9 gene transduction downregulates Wnt signal pathways.2004
Author(s)
Huang, C., Liu, D., Masuya, D., Kameyama, K., Nakashima, T., Yokomise, H., Ueno, M., Miyake, M.
-
Journal Title
Oncogene 23
Pages: 7475-7483
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Journal Article] MRP-1/CD9 gene transduction regulates the actin cytoskeleton throug the down-regulation of WAVE2.
Author(s)
Huang, CL., Ueno, M., Liu, D., Masuya, D., Nakano, J., Yokomise, H., Nakagawa, T., Miyake, M.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Clinical significance of Aminopeptidase N in Non-Small Cell LungCancer.
Author(s)
Tokuhara, T., Hattori, N., Ishida, H., Hirai, T., Higashiyama, M., Kodama, K., Miyake, M.
-
Journal Title
Clin, Cancer, Res. (in press)
Description
「研究成果報告書概要(欧文)」より
-
-
