2007 Fiscal Year Final Research Report Summary
A genetically-engineering bone marrow stem cell therapy for stroke
| Project/Area Number |
16390414
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
HONMOU Osamu Sapporo Medical University, School of Medicine, Assistant Professor (90285007)
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| Co-Investigator(Kenkyū-buntansha) |
HOUKIN Kiyohiro Sapporo Medical University, School of Medicine, Professor (90229146)
HAMADA Hirofumi Sapporo Medical University, School of Medicine, Professor (00189614)
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| Project Period (FY) |
2004 – 2007
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| Keywords | adult / gene / human / infarction / neural stem cell / stroke / transplantation |
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| Research Abstract |
Introduction : We have reported that transplantation of bone marrow cells has demonstrated therapeutic efficacy in animal models of cerebral ischemia. In the present study, we transplanted genetically-engineering bone marrow stem cells into middle cerebral artery occlusion model rats to study their potential therapeutic benefit.
Methdos : We have made five genetically-engineering bone marrow stem cells, 1) to immortalize the stem cells with hTERT gene, 2) to promote the neurotrophic effects with BDNF gene, 3) to promote the neurotrophic effects with GDNF gene, 4) to promote the angiogenetic effects with Ang-1 gene, 5) to promote the angiogenetic effects with P1GF gene. Lesion size was assessed using MR imaging and spectroscopy, and histological methods.
Results : Transplantation of genetically-engineering bone marrow stem cells reduced lesion volume. The reduction of lesion size could be assessed in vivo with MRI and was correlated with subsequent histological examination of the brain.
Conclusion : This work demonstrates that highly purified genetically-engineering bone marrow stem cells are neuroprotective and angeigenetic in cerebral ischemia models. Thus, a highly purified, expanded, and genetically-engineered cellular component of bone marrow has demonstrated the therapeutic benefits in cerebral ischemia.
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[Journal Article] Therapeutic benefits by Ang-1 gene-modified2008
Author(s)
Onda, T., Honmou, O., Harada, K, Houkin, K., Hamada, H., Koosis, J.D
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Journal Title
human mesenchvmal stem cells after cerebral ischemia. Journal of Cerebral Blood Flow & Metabolism 28
Pages: 329-340
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mesenchymal stem cells derived from peripheral blood protects against ischemia2007
Author(s)
Ukai, R., Honmou, O., Harada, K., Houkin, K, Hamada, H., Kocsis, J.D
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Journal Title
J Neurotrauma 24(3)
Pages: 508-520
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Magnetic resonance lactate and lipid signals in rat brain after middle cerebral artery occlusion model2007
Author(s)
Harada, K., Honmou, O., Liu H., Bando, M., Houkin, K., Kocsis, J.D
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Journal Title
Brain Research 1134
Pages: 206-213
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Neural differentiation potential of peripheral blood- and bone marrow-derived precursor cells2006
Author(s)
Kim, S., Honmou, O., Kato, K., Nonaka, T., Houkin, K., Hamada, H., Kocsis, J.D
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Journal Title
Brain Research 1123, 19
Pages: 27-33
Description
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[Journal Article] Intravenous administration of GDNF gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat2006
Author(s)
Horita, Y., Honmou, O., Harada, K., Houkin, K., Hamada, H., Kocsis, J.D
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Journal Title
J.Neuroscience Research 84
Pages: 1495-1504
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Neuroprotection by PlGF gene-modified human mesenchymal stem cells after cerebral ischemia2006
Author(s)
Liu, H., Honmou, O., Harada, K., Nakamura, K., Houkin, K., Hamada, H., Kocsis, J.D
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Journal Title
BRAIN 129
Pages: 2734-2745
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intravenous infusion of immortalized human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat2006
Author(s)
Honma, T., Honmou, O., Iihoshi, S., Houkin, K., Hamada, H., Kocsis, J.D
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Journal Title
Exp Neurol 199
Pages: 56-66
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Intravenous infusion of BDNF gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat2005
Author(s)
Nomura, T., Honmou, O., Harada, H., Houkin, K., Hamada, H., Kocsis, J.D
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Journal Title
Neuroscience 136
Pages: 161-169
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mesenchymal stem cells that produce neurotrophic factors reduce ischemic damage in the rat middle cerebral artery occlusion model2005
Author(s)
Kurosumi, K., Nakaniura, K., Tamiya, T., Kawano, Y., Kobune, M., Hirai, S., Uchida, H., Sasaki, K., Ito, Y., Kato, K.,Honmou, O., Houkin, K., Date, I., Hamada, H
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Journal Title
Mol. Ther 11(1)
Pages: 96-104
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Intravenous administration of immortalized human mesenchymal stem cells repairs the ischemic leisions in the rat middle cerebral artery occulusion model2004
Author(s)
Honmou, O., Iihoshi, S., Honma, T., Harada, K., Hamada, H., Houkin K., Kocsis, JD
Organizer
34th Annual Meeting of Society for Neuroscience
Place of Presentation
San Diego
Year and Date
20041023-27
Description
「研究成果報告書概要(欧文)」より
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