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2006 Fiscal Year Final Research Report Summary

Research and Development for Fabrication of 3D-structured Tissue-engineered Cartilage through Nino-molecular Technologies

Research Project

Project/Area Number 16390431
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionThe University of Tokyo

Principal Investigator

HOSHI Kazuto  The University of Tokyo, Faculty of Medicine, Visiting Associate Professor, 医学部附属病院, 寄付講座教員(客員助教授) (30344451)

Co-Investigator(Kenkyū-buntansha) ITAKA Keishi  The University of Tokyo, The graduate school of Medicine, Project Researcher, 大学院医学系研究科, 研究拠点特任教員 (60292926)
KAWAGUCHI Hiroshi  The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (40282660)
NAKAMURA Kozo  The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (60126133)
OGASAWARA Toru  The University of Tokyo, Faculty of Medicine, Project Researcher, 医学部附属病院, 寄付講座教員 (20359623)
TEI Yuichi  The University of Tokyo, Faculty of Medicine, Project Researcher, 医学部附属病院, 寄付講座教員 (30345053)
Project Period (FY) 2004 – 2006
Keywordsnanotechnology / tissue engineering / chondrocyte / hydrogel / soluble factor / 3D culture / proliferation / differentiation
Research Abstract

Cartilage is a major component that consists of motor and supportive tissues. Because the cartilaginous tissues suffer from various diseases and disorders including trauma, ageing diseases, inflammation and congenital anomaly, the regeneration and reconstruction of cartilage is the most important topic in the orthopaedic fields. Although the cartilage tissue engineering has gradually prevailed as autologous chondrocyte transplantation, 3D-structured tissue-engineered cartilage are needed in order to broaden the indication range of the cartilage tissue engineering for the more severe cartilage diseases such as end stage of osteoarthritis and congenital anomaly of cartilage. Otherwise, the nanotechnology has remarkably progressed, which can control the matter on a scale smaller than 1 micrometer and fabricate multipotent prolymers. In the present project, we attempted to apply the nanotechnology for the proliferation of chondrocytes in vitro and the fabrication of 3D-structured tissue-engineered cartilage.
In order to prevent dedifferentiation of cultured chondrocytes, 3D culture is useful. However, the proliferation culture of chondrocytes under 3D condition had not been reported. In the present project, we attempted to use hollow fibers for the purpose of making the 3D proliferation culture system. We also investigated the combination of soluble factors that redifferentiate dedifferentiated chondrocytes, in order to solve the problem that the chondrocytes are dedifferentiated during the proliferation culture. As results, the combination of BMP-2, insulin and T3 was revealed to induce ideal redifferentiation. Moreover, we examined the novel materials including the scaffolds which synthetic peptides possess no risk of cross-contamination and the functional micelles for gene transfection.
These results would provide useful information for the clinical application of cartilage tissue engineering.

  • Research Products

    (16 results)

All 2007 2006 2005 2004 Other

All Journal Article (14 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials.2006

    • Author(s)
      Yamaoka H et al.
    • Journal Title

      J Biomed Mater Res A 78A(1)

      Pages: 1-11

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Erk pathways negatively regulate matrix mineralization2006

    • Author(s)
      Kono SJ et al.
    • Journal Title

      Bone 40(1)

      Pages: 68-74

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Contribution of runt-related transcription factor 2 to the pathogenesis of osteoarthritis in mice after induction of knee joint instability2006

    • Author(s)
      Kamekura S et al.
    • Journal Title

      Arthritis Rheum 54(8)

      Pages: 2462-2470

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials2006

    • Author(s)
      Yamaoka H et al.
    • Journal Title

      J Biomed Mater Res A. 78A(1)

      Pages: 1-11

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Erk pathways negatively regulate matrix mineralization2006

    • Author(s)
      Kono SJ et al.
    • Journal Title

      Bone. 40(1)

      Pages: 68-74

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Contribution of runt-related transcription factor 2 to the pathogenesis of osteoarthritis in mice after induction of knee joint instability2006

    • Author(s)
      Kamekura S et al.
    • Journal Title

      Arthritis Rheum. 54(8)

      Pages: 2462-2470

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Suppression of adjuvant-induced arthritic bone destruction by cyclooxygenase-2 selective agents with and without inhibitory potency against carbonic anhydrase II2006

    • Author(s)
      Katagiri M et al.
    • Journal Title

      J Bone Miner Res. 21(2)

      Pages: 219-227

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regulation of bone formation by adiponectin through autocrine/paracrine and endocrine pathways2006

    • Author(s)
      Shinoda Y
    • Journal Title

      J Cell Biochem. 99(1)

      Pages: 196-208

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Synergistic Effects of FGF-2 with Insulin or IGF-I on the Proliferation of Human Auricular Chondrocytes2005

    • Author(s)
      Takahashi T et al.
    • Journal Title

      Cell Transplant 14

      Pages: 683-693

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Synergistic Effects of FGF-2 with Insulin or IGF-I on the Proliferation of Human Auricular Chondrocytes2005

    • Author(s)
      Takahashi T et al.
    • Journal Title

      Cell Transplant. 14

      Pages: 683-693

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Supramolecular nanocarrier of siRNA from PEG-based block catiomer carrying diamine side chain with distinctive pKa directed to enhance intracellular gene silencing2004

    • Author(s)
      Itaka K et al.
    • Journal Title

      J Am Chem Soc 126(42)

      Pages: 13612-13613

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Supramolecular nanocarrier of siRNA from PEG-based block catiomer carrying diamine side chain with distinctive pKa directed to enhance intracellular gene silencing2004

    • Author(s)
      Itaka K et al.
    • Journal Title

      J Am Chem Soc. 126(42)

      Pages: 13612-13613

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Three-dimensional microenvironments retain chondrocyte phenotypes during proliferation culture

    • Author(s)
      Takahashi T et al.
    • Journal Title

      Tissue Eng (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Three-dimensional microenvironments retain chondrocyte phenotypes during proliferation culture

    • Author(s)
      Takahashi T et al.
    • Journal Title

      Tissue Eng (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] バイオミネラリゼーションとそれに倣う新機能材料の創製2006

    • Author(s)
      星 和人 ほか
    • Total Pages
      308
    • Publisher
      シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 脱分化型軟骨細胞の軟骨細胞への再分化培地2007

    • Inventor(s)
      星 和人ほか
    • Industrial Property Rights Holder
      星 和人ほか
    • Industrial Property Number
      米国出願PCT/JP2005-015280
    • Filing Date
      2007-02-16
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2008-05-27  

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