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2006 Fiscal Year Final Research Report Summary

The Assessment of gene therapy for bone metastatic lesion of prostate cancer, using Positron Emission Tomography.

Research Project

Project/Area Number 16390464
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionHyogo College of Medicine (2005-2006)
Kobe University (2004)

Principal Investigator

GOTOH Akinobu  Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (70283885)

Co-Investigator(Kenkyū-buntansha) SENDA Michio  Biomedical Research and Innovation, Image-baseMedicine, Chief Director, 映像医療研究部, 部長 (00216558)
SHIRAKAWA Toshiro  Kobe University School of Medicine, International Center for Medical Research and Treatment, Associate Professor, 医学部, 助教授 (70335446)
Project Period (FY) 2004 – 2006
KeywordsPET / gene therapy / prostate cancer / [^<18>F]FDG / [^<18>F]FHBG
Research Abstract

To evaluate the therapeutic effect of the gene therapy for prostate cancer, we performed Positron Emission Tomography (PET) which employed 18F-deoxyglucose ( [18F]FDG) as a tracer, and investigated accumulation of [18F]FDG in tumor. First, we inoculated PC-3 which is a human prostate cancer cell line with Balb/C nude mouse, we formed a subcutaneous or bone tumor of PC-3. After the tumor diameter was 3-5mm (4 weeks later in bone tumor), Ad-OC-TK was injected directly into the subcutaneous tumor or the bone tumor lesion. The doses used were 2.5 x 10^9 and 2.5 x 10^<10> plaque-forming units (PFU) on Day 1 and 8 and we gave VAL daily for 21 d. After 21 d, mice were anesthetized, and injected via tail vein with [18F] FDG (5.3MBq). After lh, we sacrificed mice, and evaluated accumulation of [18F] FDG in tumor. We demonstrated low accumulation of [18F] FDG in tumor treated by gene therapy compared with no treatment. This result suggested that prostate cancer cells treated by gene therapy were low glucose rate of utilization and it was capable of assessing the therapeutic effect of the gene therapy by observing accumulation of [18F] FDG. Furthermore, we similarly tested the transduction effect of HSV-TK gene by injecting 9-[(4-[18F]-fluoro-3-hydroxymethylbuty1)guanine (FHBG) via tail vein.
High accumulation of [18F] FHBG was observed in tumor treated by gene therapy compared with no treatment. This result suggested the possibility of assessing the transduction effect of HSV-TK gene by observing accumulation of [18F] FHBG.

  • Research Products

    (22 results)

All 2007 2006 2005

All Journal Article (22 results)

  • [Journal Article] Clinical value of manual fusion of PET and CT images in patients with suspected recurrent colorectal cancer.2007

    • Author(s)
      Nakamoto Y
    • Journal Title

      AJR Am J Roentgenol. 第188巻・第1号

      Pages: 257-267

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Induction of immunological antitumor effects by adenovirus-mediated gene transfer of B7-1 in a murine squamous cell carcinomacell line.2007

    • Author(s)
      Hoshitani Y.
    • Journal Title

      Arch Otolaryngol Head Neck Surg. 第133巻・第3号

      Pages: 270-275

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Carrier cell-mediated delivery of a replication-competent adenovirus for cancer gene therapy.2007

    • Author(s)
      Hamada K.
    • Journal Title

      Mol Ther. 第15巻・第6号

      Pages: 1121-1128

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Clinical value of manual fusion of PET and CT images in patients with suspected recurrent colorectal cancer.2007

    • Author(s)
      Nakamoto Y., et al.
    • Journal Title

      AJR. Am. J. Roentgenol. 188(1)

      Pages: 257-267

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Induction of immunological antitumor effects by adenovirus-mediated gene transfer of B7-1 in a murine squamous cell carcinoma cell line.2007

    • Author(s)
      Hoshitani Y., et al.
    • Journal Title

      Arch Otolaryngol Head Neck Surg. 133(3)

      Pages: 270-5

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Carrier cell-mediated delivery of a replication-competent adenovirus for cancer gene therapy.2007

    • Author(s)
      Hamada K., et al.
    • Journal Title

      Mol. Ther. 15(6)

      Pages: 1121-28

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Progress report on phase I/II clinical trial of Ad-OC-TK plus VAL therapy for metastatic or locally recurrent prostate cancer : Initial experience at Kobe University.2006

    • Author(s)
      Hinata N.
    • Journal Title

      Int J Urol. 第13巻・第6号

      Pages: 834-837

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Performance characteristics of a new 3-dimensional continuous-emission and spiral-transmission high-sensitivity and high-resolution PET camera evaluated with the NEMA NU 2-2001 standard.2006

    • Author(s)
      Matsumoto K.
    • Journal Title

      J Nucl Med. 第47巻・第1号

      Pages: 83-90

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 再燃前立腺癌に対する治療戦略 : 遺伝子治療の現状と展望2006

    • Author(s)
      白川利朗
    • Journal Title

      Urology View 第4巻・第1号

      Pages: 71-75

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Accuracy of attenuation coefficient obtained by 137Cs single-transmission scanning in PET : comparison with conventional germanium line source.2006

    • Author(s)
      Matsumoto K.
    • Journal Title

      Nippon Hoshasen Gijutsu Gakkai Zasshi 第62巻・第2号

      Pages: 289-296

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 泌尿器科癌に対する遺伝子治療2006

    • Author(s)
      後藤章暢
    • Journal Title

      臨床泌尿器科 第60巻・第5号

      Pages: 265-271

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Progress report on phase I/II clinical trial of Ad-OC-TK plus VAL therapy for metastatic or locally recurrent prostate cancer : Initial experience at Kobe University.2006

    • Author(s)
      Hinata N., et al.
    • Journal Title

      Int. J. Urol. 13(6)

      Pages: 834-7

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Performance characteristics of a new 3-dimensional continuous-emission and spiral-transmission high-sensitivity and high-resolution PET camera evaluated with the NEMA NU 2-2001 standard.2006

    • Author(s)
      Matsumoto K.
    • Journal Title

      J Nucl Med. 47(1)

      Pages: 83-90

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Accuracy of attenuation coefficient obtained by 137Cs single-transmission scanning in PET : comparison with conventional germanium line Source.2006

    • Author(s)
      Matsumoto K.
    • Journal Title

      Nippon Hoshasen Gijutsu Gakkai Zasshi. 62(2)

      Pages: 289-96

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] An intra-operative positron probe with background rejection capability for FDG-guided surgery.2005

    • Author(s)
      Yamamoto S
    • Journal Title

      Ann Nucl Med. 第19巻・第1号

      Pages: 23-28

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ホルモン抵抗性前立腺癌転移巣に対する治療法について2005

    • Author(s)
      後藤 章暢
    • Journal Title

      泌尿器科紀要 第51巻・第2号

      Pages: 75-79

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Recombinant interleukin-2 enhanced the antitumor effect of ADV/RSV-HSV-tk/ACV therapy in a murine bladder cancer model.2005

    • Author(s)
      Terao S.
    • Journal Title

      Anticancer Res. 第25巻・第4号

      Pages: 2757-2760

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A replication-selective adenoviral vector for head and neck cancers.2005

    • Author(s)
      Tanaka H.
    • Journal Title

      Arch Otolaryngol Head Neck Surg. 第131巻・第7号(訂正第10号)

      Pages: 630-634(訂正920)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An intra-operative positron probe with background rejection capability for FDG-guided surgery.2005

    • Author(s)
      Yamamoto S., et al.
    • Journal Title

      Ann. Nucl. Med. 19(1)

      Pages: 23-28

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Gene Therapy2005

    • Author(s)
      Gotoh A.
    • Journal Title

      Hinyokika Kiyo 51(2)

      Pages: 75-79

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Recombinant interleukin-2 enhanced the antitumor effect of ADV/RSV-HSV-tk/ACV therapy in a murine bladder cancer model.2005

    • Author(s)
      Terao S., et al.
    • Journal Title

      Anticancer Res. 25(4)

      Pages: 2757-60

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A replication-selective adenoviral vector for head and neck cancers.2005

    • Author(s)
      Tanaka H.
    • Journal Title

      Arch Otolaryngol Head Neck Surg. 131(7): 630-4, Erratum in 131(10)

      Pages: 920

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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