2005 Fiscal Year Final Research Report Summary
Isolation and identification of bone morphogentic protein and its potentiator from bovine serum
| Project/Area Number |
16390533
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | Saitama Medical School |
|---|
Principal Investigator |
KATAGIRI Takenobu Saitama Medical School, School of Medicine, Associate Professor, 医学部, 助教授 (80245802)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUDA Tatsuo Saitama Medical School, School of Medicine, Professor, 医学部, 教授 (90014034)
KAMIJO Ryutaro Showa University, School of Dentistry, Professor, 歯学部, 教授 (70233939)
FUKUDA Toru Saitama Medical School, School of Medicine, Research Associate, 医学部, 助手 (20301492)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Keywords | serum / protein / osteoblast differentiation / bone formation / platelet |
|---|
| Research Abstract |
We purified a protein factor, which potentiates osteoblast differentiation, from fetal bovine serum. Although this factor itself did not show such stimulatory activity, it stimulated the osteoblast differentiation in the presence of small amount of bone morphogenetic proteins (BMPs), suggesting that this factor is a BMP potentiator. This factor is derived from platelets. Deficient mice in this factor expression showed delayed bone fracture hearing. In contrast, the fracture hearing was accelerated in transgenic mice of this factor. Moreover, we found a BMP-like factor in fetal bovine serum and isolated this activity using a series of column chromatography. The biological activity of this serum BMP was blocked by dominant negative form of BMP receptor and Noggin, a BMP antagonist. LC-MS/MS analysis indicated that the highly purified fraction contained a conserved sequence of a mature BMP. These results indicate that serum factors play a important role in bone formation. Moreover, we found that sulfated polysaccharides such as heparin and heparan sulfate also potentiated the biological activities of BMPs. When BMP was implanted together with heparin in mice, the ectopic bone-inducing activity of BMP in vivo was also potentiated by heparin. Taken together, it was suggested that BMPs and their potentiators in serum positively regulate bone formation in vertebrates.
|
