2006 Fiscal Year Final Research Report Summary
REGENERATION OF SALIVARY GLAND USING TISSUE ENGINEERING AND GROWTH FACTORS
| Project/Area Number |
16390584
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokyo (2006)
Nagoya University (2004-2005) |
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Principal Investigator |
KAGAMI Hideaki THE UNIVERSITY OF TOKYO, THE INSTITUTE OF MEDICAL SCIENCE, Visiting Associate Professor, 医科学研究所, 客員助教授 (80242866)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Minoru NAGOYA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, Professor, 大学院・医学系研究科, 教授 (00151803)
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| Project Period (FY) |
2004 – 2006
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| Keywords | salivary gland / regeneration / cell culture / tissue engineering / gene analysis / DNA microarray / cell therapy / differential display |
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| Research Abstract |
Salivary gland atrophy is a serious clinical problem for elderly and patients who undergo irradiation. However, regeneration of salivary gland is not feasible. In this project, we have investigated the genes potentially relating to the regeneration of salivary gland. Furthermore, a possibility of cell therapy on atrophic salivary gland was investigated. Male Wistar rats (nine weeks old) were anesthetized and the unilateral duct of submandibular gland was ligated. After ductal ligation, salivary gland immediately becomes atrophic. The gland regenerates after the ligation removal. The ligation was removed after one week and the gland was harvested at 12 hours, 36 hours, and 6 days after removal of ligation. The results from DNA microarray analyses showed immediate increase of clusterin expression after removal of ligation, which returned to almost basal level after 6 days. The gene and protein expression of clusterin was confirmed by RT-PCR and immunohistochemistry, respectively. In the normal gland, clusterin was equally distributed but limited in the cytosol of most of the parenchyma. After ductal ligation and removal, clusterin immediately accumulated intra-luminal space and strong expression was continuously observed along with the luminal surface of the atrophic acini until day 3. The expression was decreased after day 6 and the distribution returned to that of the normal gland. The results from this study demonstrated the dynamic change of this protein during regeneration process of rat submandibular gland, though the possible roles yet to be known. Next, we have generated a mouse model of salivary hypofunction by irradiation. Monocytes from bone marrow were harvested by centrifugation and transplanted to the atrophic submanidbular gland. In this study, various factors which affect the attachment and survival of transplanted cells were investigated. Furthermore, functional recovery of the atrophic salivary gland was also evaluated.
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