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2006 Fiscal Year Final Research Report Summary

Profiling of oral bacterial flora using DNA chips and its application to the risk evaluation of oral diseases of children.

Research Project

Project/Area Number 16390601
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionTohoku University

Principal Investigator

TAKAHASHI Nobuhiro  Tohoku University, Grad Sch of Dentistry, Professor, 大学院歯学研究科, 教授 (60183852)

Co-Investigator(Kenkyū-buntansha) HATA Shinji  Tohoku University, Hospital, Lecturer, 病院・講師 (20208507)
SATO Takuichi  Tohoku University, Grad Sch of Dentistry, Lecturer, 大学院歯学研究科, 講師 (10303132)
MATSUYAMA Junko  Niigata University, Grad Sch of Medical Dental Sciences, Assistant, 医歯学系, 助手 (30293236)
NAKAJO Kazuko  Tohoku University, Grad Sch of Dentistry, Assistant, 大学院歯学研究科, 助手 (40374946)
WASHIO Jumpei  Tohoku University, Grad Sch of Dentistry, Assistant, 大学院歯学研究科, 助手 (20400260)
Project Period (FY) 2004 – 2006
KeywordsDNA chips / 16S ribosomal RNA / DNA probe / DNA primers / Oral bacteria
Research Abstract

Oral biofilms have been reported to be associated with oral infections such as dental caries and periodontitis. And also, oral biofilms are microbial ecosystems which contain various amounts and sorts of microorganisms, and thus controlling these microbial florae are thought to be one of the ways toward prevention of these oral infections. The aim of this study was to establish DNA micro arrays system to detect oral infections-associated bacteria utilizing species-specific DNA probes of 16S ribosomal RNA genes. Twenty dentinal endodontic lesions-associated bacteria including mutans streptococci and twenty-three periodontitis-associated bacteria including Porphyromonas gingivalis, Tannerella forsythia and Mogibacterium timidum were selected in this study, and the specificity and sensitivity of the DNA micro arrays were tested. In addition, we have been planning to compare the DNA micro arrays system with the direct-& real-time PCR on their specificities and sensitivities utilizing one-hundred subgingival plaque biofilms for the present.

  • Research Products

    (8 results)

All 2006 2005 2004

All Journal Article (8 results)

  • [Journal Article] Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis2006

    • Author(s)
      Nakajo K
    • Journal Title

      Oral Microbiol Immunol 21(5)

      Pages: 283-288

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains, and its biochemical mechanism2006

    • Author(s)
      Miyasawa-Hori H
    • Journal Title

      Oral Microbiol Immunol 21(4)

      Pages: 201-205

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis.2006

    • Author(s)
      Nakajo K
    • Journal Title

      Oral Microbiol Immunol 21(5)

      Pages: 283-288

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains, and its biochemical mechanism.2006

    • Author(s)
      Miyasawa-Hori H
    • Journal Title

      Oral Microbiol Immunol 21(4)

      Pages: 201-205

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hydrogen sulfide-producing bacteria in tongue coating and their relationship with oral malodour.2005

    • Author(s)
      Washio J
    • Journal Title

      J Med Microbiol 54(9)

      Pages: 889-895

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Quantification and detection of bacteria from postoperative maxillary cyst by polymerase chain reaction.2005

    • Author(s)
      Yamaura M
    • Journal Title

      Oral Microbiol Immunol 20(6)

      Pages: 333-338

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects.2004

    • Author(s)
      Mayanagi G
    • Journal Title

      Oral Microbiol Immunol 19(6)

      Pages: 379-385

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A method for mapping the distribution pattern of cariogenic streptococci within dental plaque in vivo.2004

    • Author(s)
      Kato K
    • Journal Title

      Caries Res 38(5)

      Pages: 448-453

    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2008-05-27  

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