2007 Fiscal Year Final Research Report Summary
Clarification of the Mechanisms that Causes Internal Derangement of the Temporomandibular Joint
| Project/Area Number |
16390609
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
MIZOGUCHI Itaru Health Sciences University of Hokkaido, Department of Dentistry, Professor (20200032)
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| Co-Investigator(Kenkyū-buntansha) |
TAKUMA Taishin Health Sciences University of Hokkaido, Department of Dentistry, Professor (40095336)
SASANO Yasuyuki Tohoku University, Department of Dentistry, Professor (30196191)
飯嶋 雅弘 北海道医療大学, 歯学部, 准教授 (20305915)
OKAYAMA Miki Health Sciences University of Hokkaido, Department of Dentistry, Assistant (30382500)
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| Project Period (FY) |
2004 – 2007
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| Keywords | Temporomandibular joint / Extracellular matrix / Proteoglycans / Collagens / RT-PCR / Western Blotting |
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| Research Abstract |
To analyse the effects of mechanical environments on gene and protein expressions of major extracellular matrix components in rat temporomandibular joint (TMJ), expression and localization of the core proteins of proteoglycans in the TMJ discs during postnatal development were examined, using Western blotting, immunohistochemistry and rapid-cycle real-time RT-PCR (Real-time Polymerase Chain Reaction). The obtained results were followings; 1. Quantitative RT-PCR showed that mRNA expressions of biglycan and versican were peak after birth and gradually decreased with growing, while decorin mRNA increased with growing. 2. Among four versican isoforms, V0, V1 and V3 decreased with growing, while V2 peaked at 8 weeks and then gradually decreased.
3. Western blotting showed that mRNA expression of rapidly decreased with growing, while mRNA of decorin increased. 4. An immunohistochemical study showed that biglycan was evenly distributed in the TMJ dsics throughout the growing stage. In contrast; localization of decorin and versican showed the regional differences; the stainingfor decorin and biglycan was weak in the central part of the discs and intense in the anterior and posterior peripheral attachments, which would mainly experience tensile forces. These results demonstrate that the growth-related changes and regional differences exist in the expression of proteoglycans in the TMJ discs of growing rats, which probably reflect the alteration of biomechanical environments caused by development of orofacial functions. Further study concerning the effects of food consistency would be necessary to understand mechanisms of temporomandibular joint diseases (TMD)s.
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[Journal Article] Expression and localization of versican during postnatal development of rat temporomandibular joint disc2006
Author(s)
Toriya, N., Takuma, T., Arakawa, T., Abiko, Y., Sasano, Y., Takahashi, I., Rahemtulla, F., Mizoguchi, I
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Journal Title
Histochem Cell Biol 125
Pages: 205-214
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] One novel and one recurrent mutations in PROS1 gene cause type I protein S deficiency in patients with pulmonary embolism associated with deep vein thrombosis2006
Author(s)
Mizukami, K., Nakabayashi, T., Naitoh, S., Takeda, M., Tatumi, T., Mizoguchi, I., Ieko, M., Koike, T
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Journal Title
Amer J Hematol 81
Pages: 787-797
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Protein C Sapporo(protein C Glu 25 -> Lys): a heterozygous missense mutati on in the Gla domain provides new insight into the interaction between protein C and endothelial protein C receptor2005
Author(s)
Nakabayashi T, Mizukami K, Naitoh S, Takeda M, Shikamoto Y, Nakag awa T, Kaneko H, Tartumi T, Mizo guchi I, Mizuno H, Ieko M, Koike T
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Journal Title
Thromb Haemost 94
Pages: 942-950
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Protein C Sapporo(protein C Glu 25→ Lys) : a heterozygous missense mutation in the Gla domain provides new insight into the interaction between protein C and endothelial protein C receptor2005
Author(s)
Nakabayashi, T., Mizukami, K., Naitoh, S., Takeda, M., Shikamoto, Y., Nakagawa, T., Kaneko, H., Tartumi, T., Mizoguchi, I., Mizuno, H., leko, M., Koike, T
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Journal Title
Thromb Haemost 94
Pages: 942-950
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression of versican and ADAMTS during rat tooth eruption2005
Author(s)
Sone, S., Nakamura, M., Maruya, Y., Takahashi, I., Mizoguchi, I., mayanagi, H., Sasano, Y
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Journal Title
Mol Histol 36
Pages: 281-288
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression of versican and ADAMTS1, 4, and 5 during bone development in the rat mandible and hind limb2005
Author(s)
Nakamura, M., Sone, S., Takahashi, I., Mizoguchi, I., Echigo, S., Sasano, Y
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Journal Title
J Histochem Cytochem
Pages: 1553-1562
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Signaling mechanisms involved in protease-activated receptor-1- mediated interleukin-6 production by human gingival fibroblasts2004
Author(s)
Tanaka, N., Morita, T., Nezu, A., Tanimura, A., Mizoguchi, I., Tojyo, Y
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Journal Title
J Pharmacol Exp Ther 311
Pages: 778-786
Description
「研究成果報告書概要(欧文)」より
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[Presentation] mRNA expression of small leusine-rich proteoglycans in the temporomandibular joint disc of growing rats2005
Author(s)
Kohda, N., Toriya, N., Arakawa, T., Takuma, T., Sasano, Y., Mizoguchi, I
Organizer
Congress of Japanese Orthodontic Society
Year and Date
20051016-17
Description
「研究成果報告書概要(欧文)」より
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