2005 Fiscal Year Final Research Report Summary
Elucidation of the cause of Caribbean parkinsonism and prevention in other area
| Project/Area Number |
16406004
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Environmental pharmacy
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
OHTA Shigeru Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (60160503)
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| Co-Investigator(Kenkyū-buntansha) |
KOTAKE Yaichiro Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (20335649)
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| Project Period (FY) |
2004 – 2005
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| Keywords | benzylisoguinoline / Parkinson's disease / reticuline / N-methylcoculaurine / LC / MS / MS / French West Indies / Annonaceae family |
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| Research Abstract |
In Guaderoupe, the French West Indies, there is a high incidence of atypical parkinsonism or progressive supranuclear palsy, and all of the investigated patients had taken herbal tea or tropical fruits of the Annonaceae family. Local inhabitants consume the fruits, and also drink tea made from the leaves. In the present study, we used liquid chromatography-tandem mass spectrometry (LC/MS/MS) with multiple reaction monitoring (MRM) to detect low-molecular-weight neurotoxic benzylisoquinoline derivatives in the Annonaceae family. We detected reticuline and N-methylcoculaurine in every Annona muricata sample examined, except for pulp and seed. They were not detected in sweetsop fruits. Norreticuline was not detected in any sample. These three compounds were toxic to SH-SY5Y neuroblastoma cells and inhibited mitochondrial respiratory complex I. It is possible that uptake of the benzylisoquinoline derivatives reticuline and N-methylcoculaurine and their accumulation in the brain may be related to the pathogenesis of the local endemic disease.
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