• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Final Research Report Summary

The Role of RNA Binding Protein Hu in Neuronal Differentiation

Research Project

Project/Area Number 16500206
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionKeio University

Principal Investigator

OKANO Hirotaka, James  Keio University, School of Medicine, Department of Physiology, Associate Professor, 医学部, 助教授 (90338020)

Co-Investigator(Kenkyū-buntansha) OKANO Hideyuki  Keio University, School of Medicine, Department of Physiology, Professor, 医学部, 教授 (60160694)
Project Period (FY) 2004 – 2005
KeywordsHu / RNA binding protein / paraneoplastic neurologic disorders / translational control / neuronal differentiation / hnRNP K / neural stem cell
Research Abstract

Neuronal Hu RNA binding proteins are expressed immediately after the neuronal progenitor withdraw from cell cycle and continues to be expressed in postmitotic neurons. Recent studies indicate that overexpression of Hu is sufficient to induce neuronal differentiation by binding to UTR region of several target mRNAs in mammalian nervous system. To reveal molecular mechanism underlining timing-control of neuronal differentiation through. posttranscriptional regulation by Hu, we identified Hu associating proteins. We isolated RNP complexes associated with Hu from extracts of Hu-adenovirus infected culture cells and three proteins were identified by MALDI-TOF Mass. We revealed that hnRNPK, an RNA binding protein which is one of Hu associating proteins, bound to p21 mRNA 3'UTR, known as a target gene of Hu in vitro and in vivo, and repressed its translation in both nonneuronal and neuronal cells. Recently, it was reported that p21 not only inhibits the cell cycle but also contributes as a developmental regulator that induces axons and dendrites in newborn neurons. Then we speculated that the neuronal differentiation was regulated by two RNA binding proteins, Hu and hnRNPK through post-transcriptional regulation of p21 mRNA. By reporter assay, we found that p21 expression is controlled posttranscriptionally during neuronal differentiation and this regulation was dependent on its 3'UTR. Furthermore we showed that Hu induced neurite-outgrowth, stopped the cell cycle and upregulated p21 protein production in the mouse neuroblastoma N1E-115 cells. These effects were partially inhibited by hnRNPK. Taken all together, these data would suggest that Hu and hnRNPK controlled the timing of neuronal differentiation by regulating downstream genes.

  • Research Products

    (13 results)

All 2005

All Journal Article (12 results) Book (1 results)

  • [Journal Article] Involvement of Hu and hnRNP K in neuronal differentiation through p21 mRNA post-transcriptional regulation2005

    • Author(s)
      Yano M.et al.
    • Journal Title

      J. Biol. Chem 280

      Pages: 12690-12699

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] RNA-binding protein HuD regulates neuronal cell identity and maturation.2005

    • Author(s)
      Akamatsu W. et al.
    • Journal Title

      Proc Natl Acad Sci USA. 102

      Pages: 4625-4630

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Hzf Protein Regulates Dendritic Localization and BDNF-induced Translation of Typ1 Inositol 1, 4, 5-Trisphosphate Receptor mRNA.2005

    • Author(s)
      Iijima T. et al.
    • Journal Title

      Proc Natl Acad Sci USA. 102

      Pages: 17190-17195

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Nedd9 protein, a Cas-L homologue, is upregulated and may remodel neurons after transient global ischemia.2005

    • Author(s)
      Sasaki T. et al.
    • Journal Title

      Strokle 36

      Pages: 2457-2462

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In vivo imaging of engrafted neural stem cells : its application in evaluating the optimal timing of transplantation for spinal cord injury2005

    • Author(s)
      Okada S, et al.
    • Journal Title

      FASEB J. 19

      Pages: 1839-1841

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A novel marker for Purkinje cells, KIAA0864 protein. An analysis based on a monoclonal antibody HFB-16 in developing human cerebellum.2005

    • Author(s)
      Nakamura Y. et al.
    • Journal Title

      J. Histochem. Cytochem. 53

      Pages: 423-430

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Involvement of Hu and hnRNP K in neuronal differentiation through p21 mRNA post-transcriptional regulation2005

    • Author(s)
      Yano M, Okano HJ, Okano H.
    • Journal Title

      J Biol Chem 280

      Pages: 12690-12699

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] RNA-binding protein HuD regulates neuronal cell identity and maturation2005

    • Author(s)
      Akamatsu W, Fujihara H, Mitsuhashi T, Yano M, Shibata S, Hayakawa Y, Okano HJ, Sakakibara S, Takano H, Takano T, Takahashi T, Noda T, Okano H.
    • Journal Title

      Proc Natl Acad Sci USA. 102

      Pages: 4625-4630

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hzf Protein Regulates Dendritic Localization and BDNF-induced Translation of Type1 inositol 1,4,5-Trisphosphate Receptor mRNA2005

    • Author(s)
      Iijima T, Imai T, Kimura Y, Bernstein A, Okano HJ, Yuzaki M, Okano H.
    • Journal Title

      Proc Natl Acad Sci USA. 102

      Pages: 17190-17195

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Nedd9 protein, a Cas-L homologue, is upregulated after transient global ischemia in Rats.2005

    • Author(s)
      Sasaki T, Iwata S, Okano HJ, Urasaki Y, Hamada J, Tanaka H, Dang NH, Okano H, Morimoto C.
    • Journal Title

      Stroke. 36

      Pages: 2457-2462

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vivo imaging of engrafted neural stem cells : its application in evaluating the optimal timing of transplantation for spinal cord injury.2005

    • Author(s)
      Okada S, Ishii K, Yamane J, Iwanami A, Ikegami T, Kato H, Iwamoto Y, Nakamura M, Miyoshi H, Okano HJ, Contag CH, Toyama Y, Okano H.
    • Journal Title

      FASEB J. 19

      Pages: 1839-1841

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A novel marker for Purkinje cells, KIAA0864 protein. An analysis based on a monoclonal antibody HFB-16 in developing human cerebellum.2005

    • Author(s)
      Nakamura Y, Yamamoto M, Oda E, Kanemura Y, Kodama E, Yamamoto A, Yamamoto H, Miyado K, Okano HJ, Fukagawa R, Higaki K, Yamasaki M, Okano H
    • Journal Title

      J Histochem Cytochem. 53

      Pages: 423-430

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Tissue Stem Cells; Biblogy & Applicatibns.2005

    • Author(s)
      Okano H. et al.
    • Total Pages
      383
    • Publisher
      Taylor & Francis Group, New York
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2007-12-13  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi