2006 Fiscal Year Final Research Report Summary
Study of verification and origin of a novel medullary respiratory neuron group (para-facial respiratory group)
| Project/Area Number |
16500208
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Showa University |
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Principal Investigator |
ONIMARU Hiroshi Showa Univ., School of Med., Dept. of Physiol., Associate Professor, 医学部, 助教授 (30177258)
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| Project Period (FY) |
2004 – 2006
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| Keywords | respiratory center / medulla / rat / rhythm generation / facial nerve / central chemoreceptor / optical recording / in vitro |
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| Research Abstract |
Aim of this study was to reveal cellular level entity (e.g. distribution, morphology, physiological and pharmacological characteristics) and the function of novel medullary respiratory neuron group (para-facial respiratory group, pFRG ; Onimaru ＆ Homma, 2003). For this analysis, we used the brainstem-spinal cord preparation isolated form newborn rats. The present study revealed the following : 1)The pFRG consisted predominantly of pre-inspiratory (Pre-I) neurons. 2)Cell soma of the pFRG/Pre-I neuron was located close to the ventral surface, immediately below the pia membrane, at the level of the facial nucleus. 3)Activity of the pFRG/Pre-I neurons was not inhibited by μ-opiate agonist, DAMGO. 4)Activity of the pFRG/Pre-I neurons was reflected in the facial nerve activity. 5)When the medulla was transversely cut between the pFRG and the pre-Botzinger complex (PBC), each block of the medulla could produced spontaneous rhythmic activity under the appropriate experimental conditions. 6)These rhythmic activity corresponded to the pFRG/Pre-I (in the rostral block) and PBC/inspiratory (in the caudal block) neuron activity. 7)Rhythm of the pFRG/Pre-I neurons was depressed by increasing extracellular potassium ion concentration, application of a persistent sodium channel blocker (riluzole) or lowering CO2 pressure, whereas the PBC/inspiratory activity was more resistant to these manipulations. 8)When the medulla was cut through the midline, rhythmic activity of the pFRG/Pre-I neurons was maintained independently in the right and left medulla, but PBC-inspiratory activity was stopped. These findings showed that mechanism of rhythm generation by the pFRG/Pre-I neurons are essentially different from that of PBC-inspiratory neurons and suggested that the pFRG/Pre-I neurons determine basic respiratory rhythm under normal experimental condition with normal (or lower) external potassium concentration.
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[Journal Article] Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column.2007
Author(s)
Iigaya K, Kumagai H, Onimaru H, Kawai A, Oshima N, Onami T, Takimoto C, Kamayachi T, Hayashi K, Saruta T, Itoh H.
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Journal Title
Am J Physiol Regul Integr Comp Physiol 292
Pages: R927-36
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Kawakami K Malfunction of Respiratory-Related Neuronal Activity in Na+, K+-ATPase a2 Subunit-Deficient Mice Is Attributable to Abnormal Cl- Homeostasis in Brainstem Neurons.2004
Author(s)
Ikeda K, Onimaru H, Yamada J, Inoue K, Ueno S, Onaka T, Toyoda H, Arata A, Ishikawa T, Taketo MM, Fukuda A
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Journal Title
J Neurosci 24
Pages: 10693-10701
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ma Q Tlx3 and Tlx1 are post-mitotic selector genes determining glutamatergic over GABAergic cell fates.2004
Author(s)
Cheng L, Arata A, Mizuguchi R, Qian Y, Karunaratne A, Gray PA, Arata S, Shirasawa S, Bouchard M, Luo P, Chen CL, Busslinger M, Goulding M, Onimaru H
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Journal Title
Nature Neurosci 7
Pages: 510-517
Description
「研究成果報告書概要(欧文)」より
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