2005 Fiscal Year Final Research Report Summary
Regulatory mechanism for spine actin-cytoskeleton underlying maintenance of efficacy of synaptic transmission
| Project/Area Number |
16500209
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Neuroscience in general
|
|---|
| Research Institution | National Institute for Physiological Sciences |
|---|
Principal Investigator |
FUKAZAWA Yugo National Institute for Physiological Sciences, Cerebral Research, Assistant Professor, 大脳皮質機能研究系, 助手 (60343745)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Keywords | actin cytoskeleton / sipen synapses / neuronal plasticity / long-term memory / electron microscopy |
|---|
| Research Abstract |
The effects of glutamate on postsynaptic neurons are mediated by iGluRs in the dendritic plasma membrane. The iGluRs are accumulated at postsynaptic specializations, and a modification of synaptic efficacy by changing number and/or density of AMPA type iGluR at the postsynaptic specialization may be relevant to learning and memory. To study LTP-induced changes in distributions of AMPA and NMDA type iGluRs in the postsynaptic specialization of hippocampal neurons, we employed SDS-digested FRIL, with which 2-dimensional distribution of the membrane proteins can be analyzed quantitatively at < ; 20 nm resolution, and revealed that AMPA receptors, but not NMDA receptors, are abundant on the dendritic plasma membrane and AMPA receptors at the postsynaptic specialization on the dendritic spine, but not on the dendritic shaft, are regulated more dynamically than NMDA receptors after LTP induction.
|
