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2005 Fiscal Year Final Research Report Summary

Identification of T cell epitope peptides derived from glioma antigens

Research Project

Project/Area Number 16500225
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKeio University

Principal Investigator

OHTA Shigeki  Keio University, School of Medicine, Instructor, 医学部, 助手 (20365406)

Co-Investigator(Kenkyū-buntansha) TODA Masahiro  Keio University, School of Medicine, Instructor, 医学部, 助手 (20217508)
Project Period (FY) 2004 – 2005
Keywordsglioma / antigen / CTL / immunotherapy / HSV / G207 / GL261 / GARC-1
Research Abstract

Despite several ongoing clinical trials of immunotherapies against glioma, few glioma-specific antigens recognized by cytotoxic T lymphocytes (CTLs) have been identified. We recently demonstrated that intratumoral inoculation with herpes simplex virus (HSV) as a cancer vaccine activates tumor-specific CTLs. To identify glioma antigens recognized by CTLs, we used the HSV cancer vaccine to vaccinate mice harboring a syngeneic mouse glioma cell line, GL261. From the splenocytes of the immunized mice, we generated an H-2D^b-restricted CTL line, GCL-1, that was specific for GL261. Then, a cDNA expression library generated from GL261 was screened with GCL-1, and a new gene encoding glioma antigen, GARC-1, was isolated. Sequence analysis revealed that the GARC-1 gene isolated from GL261 had a point mutation causing an amino acid change (Asp to Asn at position 81). T-cell epitope analysis revealed that the mutated peptide GARC-1_<77-85> (AALLNKLYA), but not the wild-type peptide (AALLDKLYA), was recognized by GCL-1. These results suggest that HSV cancer vaccination may be a useful method for inducing tumor-specific CTLs and identifying tumor antigens. Furthermore, this GL261/GARC-1 murine glioma model may be useful for the development of immunotherapy for brain tumors.

  • Research Products

    (37 results)

All 2006 2005 2004 Other

All Journal Article (26 results) Book (1 results) Patent(Industrial Property Rights) (10 results)

  • [Journal Article] Identification of a glioma antigen, GARC-1, using cytotoxic Tlymphocytes induced by HSV cancer vaccine.2006

    • Author(s)
      Iizuka Y, Kojima H, Kobata T, Kawase T, Kawakami Y, Toda M
    • Journal Title

      Int J Cancer 118・4

      Pages: 942-949

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of a glioma antigen, GARC-1, using cytotoxic T lymphocytes induced by HSV cancer vaccine.2006

    • Author(s)
      Iizuka Y, Kojima H, Kobata T, Kawase T, Kawakami Y, Toda M
    • Journal Title

      Int J Cancer 118(4)

      Pages: 942-949

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunohistochemical analysis of SOX6 expression in human brain tumors.2005

    • Author(s)
      Ueda R, Yoshida K, Kawase T, Kawakami Y, Toda M
    • Journal Title

      Brain Tumor Pathol 21・3

      Pages: 117-120

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of glioma-specific RFX4-E and -F isoforms and humoral immune response in patients.2005

    • Author(s)
      Matsushita H, Uenaka A, Ono T, Hasegawa K, Sato S, Koizumi F, Nakagawa K, Toda M, et al.
    • Journal Title

      Cancer Sci 96・11

      Pages: 801-809

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 免疫療法を用いた神経再生「再生医学のUP TO DATE」2005

    • Author(s)
      戸田正博
    • Journal Title

      G.I.Reasarch 13・1

      Pages: 67-72

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ヘルペスウイルスペクターを用いた遺伝子療法2005

    • Author(s)
      戸田正博
    • Journal Title

      日本臨床 63・9

      Pages: 510-514

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 悪性神経膠腫に対する抗原特異的なT細胞を誘導する樹状ワクチン療法2005

    • Author(s)
      戸田正博
    • Journal Title

      MEDICAL BRIEFS IN Brain & Nerve 14・3

      Pages: 1-2

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 難治性神経疾患に対する治療法の開発と実用化へ向けて2005

    • Author(s)
      戸田正博
    • Journal Title

      慶應医学 82・4

      Pages: 167-179

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of glioma-specific RFX4-E and -F isoforms and humoral immune response in patients.2005

    • Author(s)
      Matsushita H, Uenaka A, Ono T, Hasegawa K, Sato S, Koizumi F, Nakagawa K, Toda M, Shingo T, Ichikawa T, Noguchi Y, Tamiya T, Furuta T, Kawase T, Date I, Nakayama E
    • Journal Title

      Cancer Sci 96(11)

      Pages: 801-809

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis, and functional recovery2004

    • Author(s)
      Mikami Y, Okano H, Sakaguchi M, Nakamura M, Shimazaki T, Okano JH, Kawakami Y, Toyama Y, Toda M
    • Journal Title

      J Neurosci Res 76・4

      Pages: 453-465

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of a transcriptional factor, SOX6, in human gliomas2004

    • Author(s)
      Ueda R, Yoshida K, Kawase T, Kawakami Y, Toda M
    • Journal Title

      Brain Tumor Pathol 21・1

      Pages: 35-38

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of human tumor antigens and its implication for diagnosis and treatment of cancer2004

    • Author(s)
      Kawakami Y, Fujita T, Matsuzaki Y, Sakurai T, Tsukamoto M, Toda M, Sumimoto H
    • Journal Title

      Cancer Sci 95・10

      Pages: 784-791

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of an epigenetically silenced gene, RFX1, in human glioma cells using restriction landmark genomic scanning2004

    • Author(s)
      Ohashi Y, Ueda M, Kawase T, Kawakami Y, Toda M
    • Journal Title

      Oncogene 23・47

      Pages: 7772-7779

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 樹状細胞を用いた神経再生2004

    • Author(s)
      戸田正博
    • Journal Title

      メディカルサイエンスダイジェスト 30・11

      Pages: 456-459

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 再生医療と大学発ベンチャーの設立2004

    • Author(s)
      戸田正博
    • Journal Title

      再生医療 3・4

      Pages: 106-108

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis, and functional recovery.2004

    • Author(s)
      Mikami Y, Okano H, Sakaguchi M, Nakamura M, Shimazaki T, Okano J H, Kawakami Y, Toyama Y, Toda M
    • Journal Title

      J Neurosci Res 76(4)

      Pages: 453-465

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of a transcriptional factor, SOX6, in human gliomas.2004

    • Author(s)
      Ueda R, Yoshida K, Kawase T, Kawakami Y, Toda M
    • Journal Title

      Brain Tumor Pathol 21(1)

      Pages: 35-38

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of human tumor antigens and its implication for diagnosis and treatment of cancer.2004

    • Author(s)
      Kawakami Y, Fujita T, Matsuzaki Y, Sakurai T, Tsukamoto M, Toda M, Sumimoto H
    • Journal Title

      Cancer Sci 95(10)

      Pages: 784-791

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of an epigenetically silenced gene, RFX1, in human glioma cells using restriction landmark genomic scanning.2004

    • Author(s)
      Ohashi Y, Ueda M, Kawase T, Kawakami Y, Toda M
    • Journal Title

      Oncogene 23(47)

      Pages: 7772-7779

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunohistochemical analysis of SOX6 expression in human brain tumors.2004

    • Author(s)
      Ueda R, Yoshida K, Kawase T, Kawakami Y, Toda M
    • Journal Title

      Brain Tumor Pathol 21(3)

      Pages: 117-120

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cancer vaccine and immunotherapy for brain tumors

    • Author(s)
      Toda M, Kawakami Y
    • Journal Title

      Curr Pharm Des in press

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Development of cancer vaccine strategies for the treatment of rain tumors.

    • Author(s)
      Toda M
    • Journal Title

      New Research on Cancer Vaccines in press

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intracarotid injection of granulocyte-macrophage colony stimulating factor induces neuroprotection in a rat transient middle cerebral artery occlusion model.

    • Author(s)
      Nakagawa T, Suga S, Kawase T, Toda M
    • Journal Title

      Brain Res in press

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cancer vaccine and immunotherapy for brain tumors.

    • Author(s)
      Toda M, Kawakami Y
    • Journal Title

      Curr Pharm Des (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development of cancer vaccine strategies for the treatment of brain tumors.

    • Author(s)
      Toda M
    • Journal Title

      New Research on Cancer Vaccines (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Intracarotid injection of granulocyte-macrophage colony stimulating factor induces neuroprotection in a rat transient middle cerebral artery occlusion model.

    • Author(s)
      Nakagawa T, Suga S, Kawase T, Toda M
    • Journal Title

      Brain Res (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] グリオーマ-病態と治療-2006

    • Author(s)
      戸田正博
    • Total Pages
      274
    • Publisher
      シュプリンガー・フェアラーク東京株式会社
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 癌ワクチン2006

    • Inventor(s)
      戸田正博, 植田良, 塚田晃三
    • Industrial Property Rights Holder
      学校法人慶應義塾大学, 株式会社GBS研究所
    • Industrial Property Number
      PCT/JP2006/300764
    • Filing Date
      2006-01-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 抗原特異的T細胞誘導剤、抗体産生誘導剤、免疫増強剤、及びワクチン2006

    • Inventor(s)
      戸田正博, 村木智子, 塚田晃三, 深谷雷太
    • Industrial Property Rights Holder
      学校法人慶應義塾大学, 株式会社GBS研究所
    • Industrial Property Number
      PCT/JP2006/301283
    • Filing Date
      2006-01-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] ニューロスフェアの形成剤2006

    • Inventor(s)
      戸田正博, 大多茂樹, 矢口雅江, 岡野栄之
    • Industrial Property Rights Holder
      学校法人慶應義塾大学, 株式会社GBS研究所
    • Industrial Property Number
      PCT/JP2006/302259
    • Filing Date
      20060229
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 癌ワクチン2006

    • Inventor(s)
      戸田正博, 植田良
    • Industrial Property Rights Holder
      学校法人慶應義塾大学, 株式会社GBS研究所
    • Industrial Property Number
      特願2006-73159
    • Filing Date
      2006-03-16
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 神経損傷の治療薬2005

    • Inventor(s)
      戸田正博, 河上裕, 戸山芳明, 三上裕嗣
    • Industrial Property Rights Holder
      学校法人慶慮義蟄大学, 株式GBS研究所
    • Industrial Property Number
      特願2005-012092
    • Filing Date
      2005-01-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] ヒトグリオーマ抗原及びその調製方法2005

    • Inventor(s)
      戸田正博, 河上裕, 河瀬斌, 飯塚幸彦
    • Industrial Property Rights Holder
      学校法人慶応義塾大学, 株式会社GBS研究所
    • Industrial Property Number
      特許第3749709号
    • Acquisition Date
      2005-12-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 癌ワクチン2005

    • Inventor(s)
      戸田正博, 植田良, 塚田晃三
    • Industrial Property Rights Holder
      学校法人慶應義塾大学, 株式会社GBS研究所
    • Industrial Property Number
      特願2005-012092
    • Filing Date
      2005-01-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 抗原特異的T細胞誘導剤、抗体産生誘導剤、免疫増強剤、及びワクチン2005

    • Inventor(s)
      戸田正博, 村木智子, 塚田晃三, 深谷雷太
    • Industrial Property Rights Holder
      学校法人慶應義塾大学, 株式会社GBS研究所
    • Industrial Property Number
      特願2005-020393
    • Filing Date
      2005-01-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] ニューロスフェアの形成剤2005

    • Inventor(s)
      戸田正博, 大多茂樹, 矢口雅江, 岡野栄之
    • Industrial Property Rights Holder
      学校法人慶應義塾大学, 株式会社GBS研究所
    • Industrial Property Number
      特願2005-033698
    • Filing Date
      2005-02-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 運動機能障害評価方法とその利用方法、及びその方法に使用するための運動機能評価装置2005

    • Inventor(s)
      戸田正博, 田伏翔尚, 矢口雅江
    • Industrial Property Rights Holder
      学校法人慶應義塾大学, 株式会社GBS研究所
    • Industrial Property Number
      特願2005-144531
    • Filing Date
      2005-05-17
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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