2006 Fiscal Year Final Research Report Summary
Establishment of the screening system of vaccine candidate genes against cysticercosis using Nod-scid mice.
| Project/Area Number |
16500277
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tottori University |
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Principal Investigator |
OKAMOTO Munehiro Tottori University, Facul of Agricul., Assoc. Prof. (70177096)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Akira Asahikawa Med. Col, Facul of Med, Prof (70054020)
SHIBAHARA Toshiyuki Tottori University, Res. Cent. Biosci Tecnol, Assoc Prof (70116937)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Taenia taeniaerormis / Library Vaccine / Protective Antigen / immunity / Taeniid Cestode / Nod-Scid mouse / rat |
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| Research Abstract |
Cysticercosis, one of the most serious larval cestodiasis of medical importance in humans and of economic importance in pigs, is spreading worldwide, especially in Asia, Africa and Latin America. The trial of the vaccine development against Taenia solium cysticercosis has been made in many countries, and actually some candidate molecules have been also reported. However, the effects of vaccine used those molecules have been not always perfect. Expression-library immunization has been proposed as an effective means to screen a large number of genes of the pathogen as candidate protective molecules. In the previous study, we demonstrated the efficacy of expression-library immunization for vaccine development against T solium cysticercosis using T taeniaeformis- rat model system. In order to pick out the vaccine candidate genes, we have to check many clones. However, pigs, which are intermediate hosts of T. solim, are not easy to use for such screening test. Then the purpose of this study is to establish the screening system using Nod-Scid mouse.
Although we have created the cDNA library from adult worms in the previous study, qualoity of the cDNA library was insufficient for using the library vaccine. So we made a new cDNA library from oncospheres or eggs using the 5'and 3'race system. The quarity of the new cDNA library seemd to be sufficient for using the vaccine.
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