2005 Fiscal Year Final Research Report Summary
Effects of heat shock protein and calcineurin in hypertrophied and atrophied skeletal muscles
| Project/Area Number |
16500414
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Kumamoto University |
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Principal Investigator |
OISHI Yasuharu Kumamoto University, Faculty of Education, Associate Professor, 教育学部, 助教授 (10203704)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Heat shock protein / calcineurin / muscle hypertrophy / muscle atrophy / myosin heavy chain / Hsp72 / rat / fiber type |
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| Research Abstract |
During passed two years, I investigated the effects of heat shock proteins (HSP) and calcineurin (CaN) in hypertrophied or atrophied rat skeletal muscles. In clenbuterol injected rat hindlimb muscles, which occurred hypertrophy and the shift of fiber phenotype from slow-to-fast, Hsp72 was decreased in the slow soleus muscle, where the CaN was increased. Functionally overload (FO) of fast plantaris muscle by cutting the tendons of its synergists induced muscle fiber hypertrophy and the shift of fiber phenotype from fast-to-slow. Hsp72 was also upregulated in functionally overloaded plantaris concomitantly with the increase in fiber size and slow fiber phenotype. Muscle hypertrophy and the shift of fiber phenotype observed in the FO plantaris was inhibited by the treatment of cyclosporin A, a inhibitor of CaN, indicating that the CaN was related to the fiber size and phenotype. In addition, thyroid hormone (T3) treatment to the FO plantaris downregulated to the expression level of the Hsp72 and CaN.
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