Effect of exercise on regulation of protein synthesis in vivo

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 16500421
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: Sendai College
• Principal Investigator: FUJII Hisao Sendai College, Sports Science, Professor, 体育学部, 教授 (90275587)
• Co-Investigator(Kenkyū-buntansha): YOSHIZAWA Fumiaki Utsunomiya University, Bioproductive Science, Associate Profesor, 農学部, 助教授 (10269243)
• Project Period (FY): 2004 – 2005
• Keywords: Exercise / Training / Protein synthesis / Translational initiation factor / Leucine

Research Abstract

Exercise promotes protein synthesis in vivo. When we continue training, it really leads muscular hypertrophy, and the quantity and quality of muscle will improve. However, in this present it do not get a clear conclusion what kind of mechanism exercise and training and a nutrient to take after exercise is promoted by a protein synthesis in vivo. Therefore, the purpose of this study was to examine the effects of acute exercise and running training for 5 weeks on mechanism of translational initiation of protein synthesis in rat skeletal muscle.
The results were as follow :
1) Phosphorylations of 4E-BP1 and S6K1 in rat skeletal muscle were stimulated by leucine oral administration (135mg/100g body weight).
2) But Phosphorylations of two factors were not altered by acute exercise.
3) Furthermore, the styles of two factors were not changed by running training for 5 weeks.
The results of this study show that stimulative function for a protein synthesis in vivo in response to leucine intake probably is differenced from the process of the action of acute exercise and training for 5 weeks.