2006 Fiscal Year Final Research Report Summary
Research on micro environmental analysis using stacked miniaturized 3D chemical system
| Project/Area Number |
16550083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
UTSUMI Yuichi University of Hyogo, LASTI, Associate professor, 高度産業科学技術研究所, 助教授 (80326298)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEO Masahiro University of Hyogo, Graduate school of engineering, Associate professor, 工学研究科物質系工学専攻, 助教授 (40236443)
MEKAR Harutaka National institute of advanced industrial science and technology, researcher, 先端製造プロセス部門, 主任研究員 (30321681)
NEGORO Seiji University of Hyogo, Graduate school of engineering, Professor, 工学研究科物質系工学専攻, 教授 (90156159)
HATTORI Tadashi University of Hyogo, LASTI, Professor, 高度産業科学技術研究所, 教授 (70326297)
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| Project Period (FY) |
2004 – 2006
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| Keywords | environmental analysis / on chip analysis / bio analysis / micro mechanics / bio reactor / reaction flow |
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| Research Abstract |
We proposed and fabricated a new chemical reactor with a vertical fluid flow operation for the wide biochemical applications. For rapid and high yield reaction between reagents with large quantity, the filters, which possess 2,100-cylindrical through-bores with 40pm diameter in 200pm-thickness PMMA sheets, were fabricated by deep X-ray lithography using synchrotron-radiation from LIGA beamline BL2 of NewSUBARU. We tried the application of the fabricated vertical reactor to an sandwich enzyme-linked immunosorbent assay (ELISA) for the analysis of mouse immunoglobulin (IgG) and nonylphenol using ultra violet (UV) absorption spectroscopy. We succeeded to obtain calibration curve of Mouse IgG and found that the mouse IgG less than 100 ng/ml concentration was quantitatively detected within 45 min of total analytical period, which was about one third of the period required for the conventional method using micro titer plate. We investigated the possibility of the analysis methods using the proposed vertical micro reactor for competitive enzyme-linked immunosorbent assay (ELISA), which enable high sensitive detection of an endocrine disrupter nonylphenol in a series of vertical fluidic operation. In the assay using filters pretreated with 0.3 Dg/ml of anti-NP-antibody, even at 0.01ng/m1 of free NP, we still observed the inhibition of the binding (B/B_0=90%). Under this condition, calibration curve of NP was obtained at the range of 0.01-10ng/ml. This sensitivity was two orders higher than the sensitivity (5-100 ng/ml) obtained by ordinary ELISA using 96-wells-micro-titer plate and the same anti-NP-antibody.
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