2005 Fiscal Year Final Research Report Summary
Development of high sensitive method for determining amino acids and its application
| Project/Area Number |
16550084
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo University of Pharmacy and Life Sciences |
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Principal Investigator |
KUZU Fumiyo Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Professor, 薬学部, 教授 (70057371)
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| Co-Investigator(Kenkyū-buntansha) |
HAKAMATA Hideki Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Associate Professor, 薬学部, 助教授 (70284750)
KOTANI Akira Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Research Associate, 薬学部, 助手 (40318184)
TAKAHASHI Kouji Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Research Associate, 薬学部, 助手 (50366415)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Amino acid / Voltammetry / Electrochemical detection / High-Performance liquid chromatography / Trolox / Oxidative prepeak / Capillary column |
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| Research Abstract |
Voltammetric determination of amino acids using trolox solution
Voltammetry of an amino acid in dimethyl sulfoxide containing LiClO_4 and Trolox gave rise to a new peak (oxidation prepeak) resulting from the oxidation of trolox at a potential more negative than that of trolox. This phenomenon was confirmed with 22 amino acids. The prepeak height was noted to depend linearly on the concentration of the amino acid to a certain value, enabling the determination of the amino acid by measuring the prepeak height.
Development and application of HPLC with electrochemical detection for determining weak bases
An HPLC system is planed to determine amino acids by post-column mixing an amino acid with trolox, followed by electrochemical detection. The detection is based on the oxidation prepeak of trolox in an unbuffered solution in the presence of weak bases. As a first step, α-tocopherol was used in place of trolox and an HPLC system was developed. The HPLC system consisted of two pumps, a column, and a wall-jet type electrochemical cell. To check the HPLC system, a weak base chlordiazepoxide was determined by the HPLC system.
Sample preparation for metabolomics
A sample preparation that effectively extracts a set of targeted metabolites from biological samples is a key step for metabolomics. Metabolites of herbal medicines were successively extracted from rat plasma and determined.
A high sensitive method for determination of redox compounds by capillary liquid chromatography
To study metabolome, high sensitive analytical methods that enable the collection of all metabolites in a biological organism are necessary. One of the candidates is capillary liquid chromatography with electrochemical detection (CLC-ECD). We confirmed that the sensitivity of our CLC-ECD system reached 1β^<-18> mol (atto mol) level.
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