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2005 Fiscal Year Final Research Report Summary

Contribution of transposable elements to spontaneous mutation rates in medaka fish

Research Project

Project/Area Number 16570002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionNagoya University

Principal Investigator

KOGA Akihiko  Nagoya Univ., Sch.of Science, Associate professor, 大学院・理学研究科, 助教授 (80192574)

Co-Investigator(Kenkyū-buntansha) SHIMA Akihiro  Univ.Tokyo, Sch.of Frontier Sciences, Professor Emeritus, 大学院・新領域創成科学研究科, 名誉教授 (60011590)
KANAMORI Akira  Nagoya Univ., Sch.of Science, Research associate, 大学院・理学研究科, 助手 (40324389)
Project Period (FY) 2004 – 2005
KeywordsTransposon / Transposition / Spontaneous mutation / Mutation rate / Medaka / Vertebrates
Research Abstract

The purpose of this research was to obtain experimental, direct evidence for our inference that transposition of transposable elements contributes to spontaneous mutations in the medaka fish. We used the i^b albion strain, which exhibits a quasi-albino phenotype due to insertion of a copy of the Tol2 transposable element in a pigmentation gene. We made single-pair crosses of this strain and analyzed changes in chromosomal locations of Tol2 copies over generations by Southern blot analysis, obtaining an estimate of 0.65 (copies/gamete/generation) for the transposition frequency. This high frequency is comparable to those realized with other highly active elements such as the Drosophila P element. We then screened embryos for mutant phenotypes. The estimated spontaneous mutation rate was as high as 0.22 (new mutations/gamete/generation), representing a 1,000-fold increase from spontaneous mutation rates so far determined with the same organism. Molecular analysis of new mutant genes revealed them to have been caused mostly by transposition of the Tol2 element. These results are a clear example of positive correlation of the transposition frequency and the spontaneous mutation rate. The results raise the possibility that the impact of DNA-based transposable elements on vertebrate genomes may be, and may have been, larger than generally postulated.

  • Research Products

    (8 results)

All 2006 2005

All Journal Article (8 results)

  • [Journal Article] Color reversion of the albino medaka fish associated with spontaneous somatic excision of the Toll transposable element from the tyrosinase gene.2006

    • Author(s)
      Tsutsumi M, Imai S, Kyono-Hamaguchi Y, Hamaguchi S, Koga A, Hori H
    • Journal Title

      Pigment Cell Research 19・3(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Targeted reduction of the DNA methylation level with 5-azacytidine promotes excision of the medaka fish Tol2 transposable element.2006

    • Author(s)
      Iida A, Shimada A, Shima A, Takamatsu N, Hori H, Takeuchi K, Koga A
    • Journal Title

      Genetical Research 88・3(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Vertebrate DNA transposon as a natural mutator : The medaka fish Tol2 element contributes to genetic variation without recognizable traces2006

    • Author(s)
      Koga A, Iida A, Hori H, Shimada A, Shima A
    • Journal Title

      Molecular Biology and Evolution 23・9(印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Color reversion of the albino medaka fish associated with spontaneous somatic excision of the Toll transposable element from the tyrosinase gene2006

    • Author(s)
      Tsutsumi M, Imai S, Kyono-Hamaguchi Y, Hamaguchi S, Koga A, Hori H
    • Journal Title

      Pigment Cell Research 19(in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Targeted reduction of the DNA methylation level with 5-azacytidine promotes excision of the medaka fish Tol2 transposable element2006

    • Author(s)
      Iida A, Shimada A, Shima A, Takamatsu N, Hori H, Takeuchi K, Koga A
    • Journal Title

      Genetical Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Vertebrate DNA transposon as a natural mutator : The medaka fish Tol2 element contributes to genetic variation without recognizable traces2006

    • Author(s)
      Koga A, Iida A, Hori H, Shimada A, Shima A
    • Journal Title

      Molecular Biology and Evolution (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Reversion mutation of i^b oculocutaneous albinism to wild-type pigmentation in medaka fish2005

    • Author(s)
      Ida A, Takamatsu N, Hori H, Wakamatsu Y, Shimada A, Shima A, Koga A
    • Journal Title

      Pigment Cell Research 18・5

      Pages: 382-384

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reversion mutation of i^b oculocutaneous albinism to wild-type pigmentation in medaka fish2005

    • Author(s)
      Iida A, Takamatsu N, Hori H, Wakamatsu Y, Shimada A, Shima A, Koga A
    • Journal Title

      Pigment Cell Research 18

      Pages: 382-384

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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