2006 Fiscal Year Final Research Report Summary
Analysis of gene regulation network associated with the ribosomes in Bacillus subtilis
| Project/Area Number |
16570005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Rikkyo University |
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Principal Investigator |
KAWAMURA Fujio Rikkyo University, College of Science, Professor, 理学部, 教授 (10126039)
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| Project Period (FY) |
2004 – 2006
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| Keywords | ribosome / Bacillus subtilis / ribosomal protein / zinc binding protein / gene duplication / rRNA op eron |
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| Research Abstract |
Complete genome sequence of over 200 microorganisms has been done and paralogous pairs of ribosomal protein genes are found in many bacterial genomes. It has been reported that genes encoding ribosomal protein L31, L33, L36 and S14 are duplicated in several bacterial genomes, and each of them can be classified into two types: one type contains a zinc-binding motif, whereas another doses not. We have shown that two types of L31 protein can be detected in ribosomes prepared from cells at different growth phases in zinc-limiting minimal medium. The RpmE is stable when it contains a zinc ion bound to its CysXXCys motif, and the expression of ytiA, coding for the zinc-lacking form of L31 protein, is, repressed by Zur. We have also shown that when purified YtiA is added to an RpmE-containing ribosome solution in vitro, YtiA-containing ribosomes are detected, thus suggesting that newly synthesized YtiA can liberate RpmE from ribosomes. From these results, we proposed that the change between the two types of L31 proein in the ribosome is triggered by changes in the concentration of zinc in the environment.
We further analyzed another paralogous pair of ribosomal protein genes, rpsN and yhzA, and proposed that the switch between their products has a different significance. rpsN is indispensable for growth and depletion of RpsN results in defective 30S subunits. YhzA can functionally replace RpsN to allow continued ribosome assembly under zinc-limiting conditions. Unlike YtiA, YhzA appeared in the ribosome at a slower rate consistent with incorporation into newly synthesized, rather than pre-existing ribosomes. These results suggested that YhzA is involved in a fale-safe system for the denovo synthesis of ribosomes under zinc-limiting conditions.
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[Journal Article] Isolation and characterization of sporulation-initiation mutation in the Bacillus sublilis prfB gene.2007
Author(s)
Asai, K., Inaoka, T., Nanamiya, H., Sadaie, Y., Ochi, K., Kawamura, F.
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Journal Title
Biosci. Biotechnol. Biochem. 71
Pages: 379-406
Description
「研究成果報告書概要(和文)」より
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[Journal Article] A fail-safe system for the ribosome under zinc-limiting conditions in Bacillus subtilis.2007
Author(s)
Natori, Y., Nanamiya, H., Akanuma, G., Kosono, S., Kudo, T., Ochi, K., Kawamura, F.
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Journal Title
Mol. Micrbiol. 63
Pages: 294-307
Description
「研究成果報告書概要(和文)」より
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[Journal Article] F. Isolation and characterization of sporulation-initiation mutation in the Bacillus subtilis prfB gene.2007
Author(s)
Asai, K., Inaoka, T., Nanamiya, H., Sadaie, Y., Ochi, K., Kawamura
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Journal Title
Biosci. Biotechnol. Biochem. 71
Pages: 397-406
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ochi, K., and Kawamura, F. A fale-safe system for the ribosome under zinc-limiting conditions in Bacillus subtilis.2007
Author(s)
Natori, Y., Nanamiya, H., Akanuma, G., Kosono, S., Kudo, T.
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Journal Title
Mol. Microbiol. 63
Pages: 294-307
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Isolated chloroplast division machinery can actively constrict after stretching.2006
Author(s)
Yoshida, Y., Kuroiwa, Misumi, O., Nishida, K., Yagisawa, F., Fujiwara, T., Nanamiya, H., Kawamura, F., Kuroiwa, T.
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Journal Title
Science 313
Pages: 1435-1438
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Construction and characterization of Bacillus subtilis deletion mutants lacking the prophase 2-trnS region.2006
Author(s)
Akanuma, G., Habu, C., Natori, Y., Murayama, R., Nanamiya, H., Kawamura, F.
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Journal Title
FEMS Microbiol. Lett.
Pages: 220-226
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Construction of Bacillus subtilis strains carrying the transcriptional bgaB fusion with the promoter region of each rrn operon and their differential transcription during spore development.2006
Author(s)
Koga, K., Ikegami, A., Nakasone, K., Murayama, R., Akanuma, G., Natori, Y., Nanamiya, H., Kawamura, F.
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Journal Title
J. Gen. Appl. Microbiol. 52
Pages: 119-124
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Functional myo-inositol catabolic genes of Bacillus subtilis natto are involved in depletion of pinitol in natto (fermented soybean).2006
Author(s)
Morinaga, T., Yamaguchi, M., Makino, Y., Nanamiya, H., Takahashi, K., Yoshikawa, H., Kawamura, F., Ashida, H., Yoshida, K.
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Journal Title
Biosci. Biotechnol. Biochem. 70
Pages: 1913-1920
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Liberation of zinc-containing L31 (RpmE) from ribosomes by its paralogous gene product, YtiA, in Bacillus subtilis.2006
Author(s)
Akanuma, G., Nanamiya, H., Natori, Y., Nomura N., Kawamura, F.
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Journal Title
J. Bacteriol 188
Pages: 2715-2720
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Nanamiya, H.,Kawamura, F., and Kuroiwa, Isolated chloroplast division machinery can actively constrict after stretching.2006
Author(s)
Yoshida, Y., Kuroiwa, H., Misumi, Nishida, K., Yagisawa, F., Fujiwara, T.
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Journal Title
Science 313
Pages: 1435-8
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Functional myo-Inositol Catabolic Genes of Bacillus subtilis Natto Are Involved in Depletion of Pinitol in Natto (Fermented Soybean).2006
Author(s)
Morinaga, T., Yamaguchi, M., Makino, Y., Nanamiya, H., Takahashi, K., Yoshikawa, H., Kawamura, F., Ashida, H., Yoshida, K.
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Journal Title
Biosci. Biotechnol. Biochem. 70
Pages: 1913-20
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Construction and characterization of Bacillus subtilis deletion mutants lacking the prophage 2-trnS region.2006
Author(s)
Akanuma, G., Habu, C., Natori, Y., Murayama, R., Nanamiya, H., Kawamura, F.
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Journal Title
FEMS Microbiol. Lett. 258
Pages: 220-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Liberation of zinc-containing L31 (RpmE) from ribosomes by its paralogous gene product, YtiA, in Bacillus subtilis.2006
Author(s)
Akanuma, G., Nanamiya, H., Natori, Y., Nomura, N., Kawamura, F.
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Journal Title
J. Bacteriol. 188
Pages: 2715-20
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of the nonA and nonB loci of Bacillus subtilis Marburg permitting the growth of SP10 phase.2004
Author(s)
Matsuoka, S., Arai, T., Murayama, R., Kawamura, F., Asai, K., Sadaie, Y.
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Journal Title
Genes Genet. Syst. 79
Pages: 311-317
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Zinc is a key factor in controlling alteration of two types of L31 protein in the Bacillus subtilis ribosome.2004
Author(s)
Nanamiya, H., Akanuma, G., Natori, Y., Murayama, R., Kosono, S., Kudo, T., Kobayashi, K., Ogasawara, N., Park, SM., Ochi, K., Kawamura, F.
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Journal Title
Mol. Microbiol. 52
Pages: 273-283
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Identification of the nonA and nonB loci of Bacillus subtilis Marburg permitting the growth of SP10 phage.2004
Author(s)
Matsuoka, S., Arai, T., Murayama, R., Kawamura, F., Asai, K., Sadaie, Y.
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Journal Title
Genes Genet. Syst. 79
Pages: 311-7
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Zinc is a key factor in controlling alternation of two types of L31 protein in the Bacillus subtilis ribosome.2004
Author(s)
Nanamiya, H., Akanuma, G., Natori, Y., Murayama, R., Kosono, S., Kudo, T., Kobayashi, K., Ogasawara, N., Park, SM., Ochi, K., Kawamura, F.
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Journal Title
Mol. Microbiol. 52
Pages: 273-83
Description
「研究成果報告書概要(欧文)」より
