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2005 Fiscal Year Final Research Report Summary

Roles of molecular chaperones for regulation of genes and assembly of photosynthetic apparatus

Research Project

Project/Area Number 16570028
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionSAITAMA UNIVERSITY

Principal Investigator

NAKAMOTO Hitoshi  Saitama University, Dept.of Biochemistry and Molecular Biology, Associate Professor, 理学部, 助教授 (30192678)

Project Period (FY) 2004 – 2005
KeywordsMolecular chaperone / Heat shock protein / IsiA / Small heat shock protein / HtpG / GroEL / Phycobilisome / Cyanobacterium
Research Abstract

1.A positive control of the cyanobacterial groESL1 transcription by a novel molecular chaperone, Orf7.5
Orf7.5 was a positive transcription factor which increases the activity of the groESL1 promoter of Synechococcus sp. PCC 7942 especially under heat shock conditions by interacting with the major sigma factor.
2.Post-transcriptional regulation of the cyanobacterial hspA heat-shock induction
The hspA gene from Synechococcus vulcanus encodes a small heat-shock protein. We showed that the hspA heat-shock induction is self-controlled post-transcriptionally.
3.Cellular functions of HspA
Comparative analyses of the wild-type Synechocystis sp. PCC 6803 and its hspA null mutant strain showed that hspA is involved in salt-stress management. The role of HspA under stress conditions was investigated comparing Synechococcus strain ECT16-1, which constitutively expresses HspA, with the reference strain ECT. HspA conferred the cyanobacterium ultrastructural stability under high temperature or intensive … More light stress.
4.Cellular functions of HtpG
We elucidated whether constitutive expression of HspA can functionally replace HtpG in Synechococcus sp. PCC 7942. It did not improve defects of an htpG mutant under stress conditions, indicating that cellular function of HtpG may differ from that of HspA.
5.Roles of molecular chaperone for the phycobilisome assembly
We showed that HspA interacts directly with phycocyanins from Synechococcus sp. PCC 7942 in vitro. We also showed a specific interaction between a linker polypeptide of phycobilisome and HtpG These results indicate that the molecular chaperones play important roles in the phycobilisome assembly by stabilizing phycocyanins and the linker polypeptide.
6.Roles of the cyanobacterial isiABC operon in protection from oxidative and heat stresses
The isiAB operon encodes CP43' and flavodoxin which form the photosynthetic apparatus. We showed that the isiABC operon plays essential roles not only under iron stress, but also under multiple stresses such as oxidative and heat stresses. Less

  • Research Products

    (16 results)

All 2006 2005 2004

All Journal Article (15 results) Book (1 results)

  • [Journal Article] Intact carboxysomes in a cyanobacterial cell visualized by Hilbert differential contrast transmission electron microscopy2006

    • Author(s)
      Kaneko, Y.
    • Journal Title

      Journal of Bacteriology 188・2

      Pages: 805-808

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Interaction of a small heat shock protein with light-harvesting cyanobacterial phycocyanins under stress conditions.2006

    • Author(s)
      Nakamoto, H.
    • Journal Title

      FEBS Letters (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Roles of the cyanobacterial isiABC operon in protection from oxidative and heat stresses.2006

    • Author(s)
      Kojima K.
    • Journal Title

      Physiologia Plantarum (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intact carboxysomes in a cyanobacterial cell visualized by Hilbert differential contrast transmission electron microscopy2006

    • Author(s)
      Kaneko, Y. et al.
    • Journal Title

      Journal of Bacteriology 188(2)

      Pages: 805-808

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Interaction of a small heat shock protein with light-harvesting cyanobacterial phycocyanins under stress conditions.2006

    • Author(s)
      Nakamoto, H., D.Honma
    • Journal Title

      FEBS Letters (In press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Roles of the cyanobacterial isiABC operon in protection from oxidative and heat stresses2006

    • Author(s)
      Kojima K. et al.
    • Journal Title

      Physiologia Plantarum (In press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ultrastructural stability under high temperature or intensive light stress conferred by a small heat shock protein in cyanobacteria.2005

    • Author(s)
      Nitta, K.
    • Journal Title

      FEBS Letters 579・5

      Pages: 1235-1242

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Constitutive expression of small heat shock protein in an htpG disruptant of the cyanobacterium Synechococcus sp. PCC 7942.2005

    • Author(s)
      Kojima, K
    • Journal Title

      Current Microbiology 50・5

      Pages: 272-276

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Post-transcriptional control of the cyanobacterial hspA heat-shock induction.2005

    • Author(s)
      Kojima K.
    • Journal Title

      Biochemical Biophysical Research Communications 331・2

      Pages: 583-588

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Constitutive expression of small heat shock protein in an htpG disruptant of the cyanobacterium Synechococcus sp. PCC 7942.2005

    • Author(s)
      Kojima, K., H.Nakamoto
    • Journal Title

      Current Microbiology 50(5)

      Pages: 272-276

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ultrastructural stability under high temperature or intensive light stress conferred by a small heat shock protein in cyanobacteria.2005

    • Author(s)
      Nitta, K. et al.
    • Journal Title

      FEBS Letters 579(5)

      Pages: 1235-1242

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Post-transcritional control of the cyanobacterial hspA heat-shock induction.2005

    • Author(s)
      Kojima K..H.Nakamoto
    • Journal Title

      Biochemical Biophysical Research Communications 331(2)

      Pages: 583-588

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Both CP43' and flavodoxin play a role in protection from heat and oxidative stresses.2005

    • Author(s)
      Kojima, K., H.Nakamoto
    • Journal Title

      Photosynthesis : Fundamental Aspects to Global Perspectives. (van der Est, A. and Bruce, D., eds.)(ACG Publishing.) Vol.2

      Pages: 709-711

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Comparative analysis of hspA mutant and wild type Synechocystis sp. PCC6803 under salt stress : Evaluation of the role of hspA in salt stress management.2004

    • Author(s)
      Asadulghani
    • Journal Title

      Archives of Microbiology 182・6

      Pages: 487-497

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Comparative analysis of hspA mutant and wild type Synechocystis sp. PCC 6803 under salt stress : Evaluation of the role of hspA in salt stress management.2004

    • Author(s)
      Asadulghani et al.
    • Journal Title

      Archives of Microbiology 182(6)

      Pages: 487-497

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Both CP43' and flavodoxin play a role in protection from heat and oxidative stresses. In : van der Est, A. and D. Bruce, eds., Photosynthesis : Fundamental Aspects to Global Perspectives.2005

    • Author(s)
      Kojima, K.
    • Total Pages
      709-711
    • Publisher
      ACG Publishing
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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