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2005 Fiscal Year Final Research Report Summary

Analyses of plant morphogenesis by cytoskeleton and vacuole.

Research Project

Project/Area Number 16570050
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionThe University of Tokyo

Principal Investigator

HASEZAWA Seiichiro  The University of Tokyo, Graduate School of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (40172902)

Project Period (FY) 2004 – 2005
Keywordsmicrotubule / actin microfilament / vacuole / GFP / tobacco BY-2 cell / Arabidopsis cultured cell / three dimensional analysis / cell differentiation & morphogenesis
Research Abstract

In this study, I have developed the transgenic BY-2 cells and analyzed the dynamics of cytoskeleton and vacuole, as follows.
1) I introduced GFP-tubulin, -fimbrin and -AtVam3p into tobacco BY-2 cells and Arabidopsis Col-0 cells for visualizing microtubules (MT), actin microfilaments (MT) and vacuolar membrane (VM), respectively. Then, I succeeded in establishing the cell lines for the time-sequential observations.
2) I developed the new algorithm for three dimensional (3-D) reconstruction, using confocal images, named REANT (reconstructor and analyzer of 3-D structure).
3) I observed the developmental process of vacuoles in BY-2 mini-protoplasts, in which almost vacuoles were eliminated. The mini-protoplasts became to reorganize the large vacuoles through the tubular structures of vacuoles.
4) I performed time-sequential observations of MF dynamics during cell division, and revealed the appearance of two MF bands in metaphase. The cell plate formed exactly within the valley between the bands.
5) I succeeded in differentiating the Arabidopsis cells, expressing GFP-tubulin, into tracheary elements, and analyzed the roles of MTs in secondary wall thickening.

  • Research Products

    (14 results)

All 2006 2005 2004

All Journal Article (12 results) Book (2 results)

  • [Journal Article] Calcium ions are involved in the delay of plant cell cycle progression by abiotic stresses.2006

    • Author(s)
      Toshio Sano
    • Journal Title

      FEBS Letters 580

      Pages: 597-602

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regulation of secondary cell wall development by cortical microtubules during tracheary element differentiation in Arabidopsis cell suspension.2005

    • Author(s)
      小田 祥久
    • Journal Title

      Plant Physiology 137

      Pages: 1027-1036

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ethylene inhibits abscisic acid-induced stomatal closure in Arabidopsis.2005

    • Author(s)
      田中 洋子
    • Journal Title

      Plant Physiology 138

      Pages: 2337-2343

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Decision of spindle poles and division plane by double preprophase bands in a BY-2 cell line expressing GFP-tubulin.2005

    • Author(s)
      米田 新
    • Journal Title

      Plant and Cell Physiology 46

      Pages: 531-538

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Appearance of actin microfilament 'twin peaks' in mitosis and their function in cell plate formation, as visualized in tobacco BY-2 cells expressing GFP-fimbrin.2005

    • Author(s)
      佐野 俊夫
    • Journal Title

      The Plant Journal 44

      Pages: 595-605

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Morphometrical study of plant vacuolar dynamics in single cells using three-dimensional reconstruction from optical sections.2005

    • Author(s)
      朽名 夏麿
    • Journal Title

      Microscopy Research and Technique 68

      Pages: 296-306

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Calcium ions are involved in the delay of plant cell cycle progression by abiotic stresses.2005

    • Author(s)
      佐野 俊夫
    • Journal Title

      FEBS Letters 580

      Pages: 597-602

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulation of secondary cell wall development by cortical microtubules during tracheary element differentiation in Arabidopsis cell suspension.2005

    • Author(s)
      Yoshihisa Oda
    • Journal Title

      Plant Physiology 137

      Pages: 1027-1036

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ethylene inhibits abscisic acid-induced stomatal closure in Arabidopsis.2005

    • Author(s)
      Yoko Tanaka
    • Journal Title

      Plant Physiology 138

      Pages: 2337-2343

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Decision of spindle poles and division plane by double preprophase bands in a BY-2 cell line expressing GFP-tubulin.2005

    • Author(s)
      Arata Yoneda
    • Journal Title

      Plant & Cell Physiology 46

      Pages: 531-538

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Appearance of actin microfilament "twin peaks" in mitosis and their function in cell plate formation, as visualized in tobacco BY-2 cells expressing GFP-fimbrin.2005

    • Author(s)
      Toshio Sano
    • Journal Title

      The Plant Journal 44

      Pages: 595-605

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Morphometrical study of plant vacuolar dynamics in single cells using three-dimensional reconstruction from optical sections.2005

    • Author(s)
      Natsumaro Kutsuna
    • Journal Title

      Microscopy Research & Techniques 68

      Pages: 296-306

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Tobacco BY-2 CellsBiotechnology in Agriculture and Forestry Vol.532004

    • Author(s)
      長田 敏行
    • Total Pages
      347
    • Publisher
      Springer-Verlag Berlin Heidelberg
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Biotechnology in Agriculture and Forestry Vol.53, (Tobacco BY-2 Cells.)2004

    • Author(s)
      Toshiyuki Nagata
    • Total Pages
      347
    • Publisher
      Springer-Verlag Berlin Heidelberg
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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